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Molecular and Cellular Biology, August 2000, p. 5404-5414, Vol. 20, No. 15
Graduate Program in Genetics and Molecular
Biology,1 Graduate Program in
Biochemistry and Molecular Biology,2 and
Department of Biology,3 Emory
University, Atlanta, Georgia 30322
Received 28 February 2000/Returned for modification 17 April
2000/Accepted 9 May 2000
The impact of high levels of RNA polymerase II transcription on
mitotic recombination was examined using lys2 recombination substrates positioned on nonhomologous chromosomes. Substrates were
used that could produce Lys+ recombinants by either a
simple (noncrossover) gene conversion event or a crossover-associated
recombination event, by only a simple gene conversion event, or by only
a crossover event. Transcription of the lys2 substrates was
regulated by the highly inducible GAL1-10 promoter or the
low-level LYS2 promoter, with GAL1-10 promoter activity being controlled by the presence or absence of the Gal80p negative regulatory protein. Transcription was found to stimulate recombination in all assays used, but the level of stimulation varied
depending on whether only one or both substrates were highly transcribed. In addition, there was an asymmetry in the types of
recombination events observed when one substrate versus the other was
highly transcribed. Finally, the lys2 substrates were positioned as direct repeats on the same chromosome and were found to
exhibit a different recombinational response to high levels of
transcription from that exhibited by the repeats on nonhomologous chromosomes. The relevance of these results to the mechanisms of
transcription-associated recombination are discussed.
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Stimulation of Mitotic Recombination Events by High
Levels of RNA Polymerase II Transcription in Yeast

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Corresponding author. Mailing address: Department of
Biology, 1510 Clifton Rd., Emory University, Atlanta, GA 30322. Phone: (404) 727-6312. Fax: (404) 727-2880. E-mail:
jinks{at}biology.emory.edu.
Present address: WorldWideTesting.com, Atlanta, GA 30328.
Present address: Diazyme-General Atomics, San Diego, CA 92186.
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