This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Saxe, D.
Right arrow Articles by Jinks-Robertson, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saxe, D.
Right arrow Articles by Jinks-Robertson, S.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, August 2000, p. 5404-5414, Vol. 20, No. 15
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Stimulation of Mitotic Recombination Events by High Levels of RNA Polymerase II Transcription in Yeast

Dean Saxe,1,dagger Abhijit Datta,2,Dagger and Sue Jinks-Robertson1,2,3,*

Graduate Program in Genetics and Molecular Biology,1 Graduate Program in Biochemistry and Molecular Biology,2 and Department of Biology,3 Emory University, Atlanta, Georgia 30322

Received 28 February 2000/Returned for modification 17 April 2000/Accepted 9 May 2000

The impact of high levels of RNA polymerase II transcription on mitotic recombination was examined using lys2 recombination substrates positioned on nonhomologous chromosomes. Substrates were used that could produce Lys+ recombinants by either a simple (noncrossover) gene conversion event or a crossover-associated recombination event, by only a simple gene conversion event, or by only a crossover event. Transcription of the lys2 substrates was regulated by the highly inducible GAL1-10 promoter or the low-level LYS2 promoter, with GAL1-10 promoter activity being controlled by the presence or absence of the Gal80p negative regulatory protein. Transcription was found to stimulate recombination in all assays used, but the level of stimulation varied depending on whether only one or both substrates were highly transcribed. In addition, there was an asymmetry in the types of recombination events observed when one substrate versus the other was highly transcribed. Finally, the lys2 substrates were positioned as direct repeats on the same chromosome and were found to exhibit a different recombinational response to high levels of transcription from that exhibited by the repeats on nonhomologous chromosomes. The relevance of these results to the mechanisms of transcription-associated recombination are discussed.

* Corresponding author. Mailing address: Department of Biology, 1510 Clifton Rd., Emory University, Atlanta, GA 30322. Phone: (404) 727-6312. Fax: (404) 727-2880. E-mail: jinks{at}biology.emory.edu.

dagger Present address: WorldWideTesting.com, Atlanta, GA 30328.

Dagger Present address: Diazyme-General Atomics, San Diego, CA 92186.

Molecular and Cellular Biology, August 2000, p. 5404-5414, Vol. 20, No. 15
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Gottipati, P., Helleday, T. (2009). Transcription-associated recombination in eukaryotes: link between transcription, replication and recombination. Mutagenesis 24: 203-210 [Abstract] [Full Text]  
  • Schildkraut, E., Miller, C. A., Nickoloff, J. A. (2006). Transcription of a Donor Enhances Its Use during Double-Strand Break-Induced Gene Conversion in Human Cells. Mol. Cell. Biol. 26: 3098-3105 [Abstract] [Full Text]  
  • Ercan, S., Reese, J. C., Workman, J. L., Simpson, R. T. (2005). Yeast Recombination Enhancer Is Stimulated by Transcription Activation. Mol. Cell. Biol. 25: 7976-7987 [Abstract] [Full Text]  
  • Pyne, S., Skiena, S., Futcher, B. (2005). Copy Correction and Concerted Evolution in the Conservation of Yeast Genes. Genetics 170: 1501-1513 [Abstract] [Full Text]  
  • Sharkey, L. L., Liao, W.-l., Ghosh, A. K., Fonzi, W. A. (2005). Flanking direct repeats of hisG alter URA3 marker expression at the HWP1 locus of Candida albicans. Microbiology 151: 1061-1071 [Abstract] [Full Text]  
  • Langston, L. D., Symington, L. S. (2004). Gene targeting in yeast is initiated by two independent strand invasions. Proc. Natl. Acad. Sci. USA 101: 15392-15397 [Abstract] [Full Text]  
  • Lippert, M. J., Freedman, J. A., Barber, M. A., Jinks-Robertson, S. (2004). Identification of a Distinctive Mutation Spectrum Associated with High Levels of Transcription in Yeast. Mol. Cell. Biol. 24: 4801-4809 [Abstract] [Full Text]  
  • Nikolaidis, N., Nei, M. (2004). Concerted and Nonconcerted Evolution of the Hsp70 Gene Superfamily in Two Sibling Species of Nematodes. Mol Biol Evol 21: 498-505 [Abstract] [Full Text]  
  • Garcia-Rubio, M., Huertas, P., Gonzalez-Barrera, S., Aguilera, A. (2003). Recombinogenic Effects of DNA-Damaging Agents Are Synergistically Increased by Transcription in Saccharomyces cerevisiae: New Insights Into Transcription-Associated Recombination. Genetics 165: 457-466 [Abstract] [Full Text]  
  • Takeuchi, Y., Horiuchi, T., Kobayashi, T. (2003). Transcription-dependent recombination and the role of fork collision in yeast rDNA. Genes Dev. 17: 1497-1506 [Abstract] [Full Text]  
  • Gonzalez-Barrera, S., Garcia-Rubio, M., Aguilera, A. (2002). Transcription and Double-Strand Breaks Induce Similar Mitotic Recombination Events in Saccharomyces cerevisiae. Genetics 162: 603-614 [Abstract] [Full Text]  
  • Freedman, J. A., Jinks-Robertson, S. (2002). Genetic Requirements for Spontaneous and Transcription-Stimulated Mitotic Recombination in Saccharomyces cerevisiae. Genetics 162: 15-27 [Abstract] [Full Text]  
  • Chavez, S., Garcia-Rubio, M., Prado, F., Aguilera, A. (2001). Hpr1 Is Preferentially Required for Transcription of Either Long or G+C-Rich DNA Sequences in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 7054-7064 [Abstract] [Full Text]  
  • Wai, H., Johzuka, K., Vu, L., Eliason, K., Kobayashi, T., Horiuchi, T., Nomura, M. (2001). Yeast RNA Polymerase I Enhancer Is Dispensable for Transcription of the Chromosomal rRNA Gene and Cell Growth, and Its Apparent Transcription Enhancement from Ectopic Promoters Requires Fob1 Protein. Mol. Cell. Biol. 21: 5541-5553 [Abstract] [Full Text]  
  • Malagon, F., Aguilera, A. (2001). Yeast spt6-140 Mutation, Affecting Chromatin and Transcription, Preferentially Increases Recombination in Which Rad51p-Mediated Strand Exchange Is Dispensable. Genetics 158: 597-611 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»