Previous Article | Next Article ![]()
Molecular and Cellular Biology, August 2000, p. 5749-5757, Vol. 20, No. 15
Department of Biological Sciences, University
of Pittsburgh, Pittsburgh, Pennsylvania 15260
Received 1 February 2000/Returned for modification 2 March
2000/Accepted 27 April 2000
The J domain of simian virus 40 (SV40) large T antigen is required
for efficient DNA replication and transformation. Despite previous
reports demonstrating the promiscuity of J domains in heterologous
systems, results presented here show the requirement for specific
J-domain sequences in SV40 large-T-antigen-mediated activities. In
particular, chimeric-T-antigen constructs in which the SV40 T-antigen J
domain was replaced with that from the yeast Ydj1p or Escherichia
coli DnaJ proteins failed to replicate in BSC40 cells and did not
transform REF52 cells. However, T antigen containing the JC virus J
domain was functional in these assays, although it was less efficient
than the wild type. The inability of some large-T-antigen chimeras to
promote DNA replication and elicit cellular transformation was not due
to a failure to interact with hsc70, since a nonfunctional chimera,
containing the DnaJ J domain, bound hsc70. However, this nonfunctional
chimeric T antigen was reduced in its ability to stimulate hsc70 ATPase
activity and unable to liberate E2F from p130, indicating that
transcriptional activation of factors required for cell growth and DNA
replication may be compromised. Our data suggest that the T-antigen J
domain harbors species-specific elements required for viral activities in vivo.
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Species-Specific Elements in the Large T-Antigen J
Domain Are Required for Cellular Transformation and DNA Replication by
Simian Virus 40
*
Corresponding author. Mailing address: Department of
Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260. Phone: (412) 624-4350. Fax: (412) 624-4759. E-mail:
pipas+{at}pitt.edu.
This article has been cited by other articles:
| J. Bacteriol. | J. Virol. | Eukaryot. Cell |
|---|
| Microbiol. Mol. Biol. Rev. | Clin. Vaccine Immunol. | All ASM Journals |
|---|