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Molecular and Cellular Biology, August 2000, p. 5865-5878, Vol. 20, No. 16
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Regulation of c-SRC Activity and Function by the Adapter Protein CAS

Mary Rose Burnham,1 Pamela J. Bruce-Staskal,1 Mary T. Harte,2 Cheryl L. Weidow,1 Amy Ma,1 Scott A. Weed,1 and Amy H. Bouton1,*

Department of Microbiology and Cancer Center, Health Sciences Center, University of Virginia, Charlottesville, Virginia 22908,1 and Biochemistry Department, Trinity College, Dublin 2, Ireland2

Received 16 August 1999/Returned for modification 11 October 1999/Accepted 7 May 2000

SRC family kinases play essential roles in a variety of cellular functions, including proliferation, survival, differentiation, and apoptosis. The activities of these kinases are regulated by intramolecular interactions and by heterologous binding partners that modulate the transition between active and inactive structural conformations. p130CAS (CAS) binds directly to both the SH2 and SH3 domains of c-SRC and therefore has the potential to structurally alter and activate this kinase. In this report, we demonstrate that overexpression of full-length CAS in COS-1 cells induces c-SRC-dependent tyrosine phosphorylation of multiple endogenous cellular proteins. A carboxy-terminal fragment of CAS (CAS-CT), which contains the c-SRC binding site, was sufficient to induce c-SRC-dependent protein tyrosine kinase activity, as measured by tyrosine phosphorylation of cortactin, paxillin, and, to a lesser extent, focal adhesion kinase. A single amino acid substitution located in the binding site for the SRC SH3 domain of CAS-CT disrupted CAS-CT's interaction with c-SRC and inhibited its ability to induce tyrosine phosphorylation of cortactin and paxillin. Murine C3H10T1/2 fibroblasts that expressed elevated levels of tyrosine phosphorylated CAS and c-SRC-CAS complexes exhibited an enhanced ability to form colonies in soft agar and to proliferate in the absence of serum or growth factors. CAS-CT fully substituted for CAS in mediating growth in soft agar but was less effective in promoting serum-independent growth. These data suggest that CAS plays an important role in regulating specific signaling pathways governing cell growth and/or survival, in part through its ability to interact with and modulate the activity of c-SRC.

* Corresponding author. Mailing address: Department of Microbiology, Box 800 734, Health Sciences Center, University of Virginia, Charlottesville, VA 22908. Phone: (804) 924-2513. Fax: (804) 982-1071. E-mail: ahb8y{at}virginia.edu.

Molecular and Cellular Biology, August 2000, p. 5865-5878, Vol. 20, No. 16
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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