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Molecular and Cellular Biology, August 2000, p. 6051-6061, Vol. 20, No. 16
Lineberger Comprehensive Cancer
Center,1 Program in Oral
Biology,2 Department of
Microbiology-Immunology,3 and Department
of Biochemistry and Molecular Biophysics,4
University of North Carolina at Chapel Hill, Chapel Hill, North
Carolina 27599-7295, and Department of Molecular Genetics,
M. D. Anderson Cancer Center, University of Texas, Houston,
Texas 770305
Received 10 November 1999/Returned for modification 13 December
1999/Accepted 22 May 2000
Scaffold molecules interact with multiple effectors to elicit
specific signal transduction pathways. CIITA, a non-DNA-binding regulator of class II major histocompatibility complex (MHC) gene transcription, may serve as a transcriptional scaffold. Regulation of
the class II MHC promoter by CIITA requires strict spatial-helical arrangements of the X and Y promoter elements. The X element binds RFX
(RFX5/RFXANK-RFXB/RFXAP) and CREB, while Y binds NF-Y/CBF (NF-YA,
NF-YB, and NF-YC). CIITA interacts with all three. In vivo analysis
using both N-terminal and C-terminal deletion constructs identified
critical domains of CIITA that are required for interaction with NF-YB,
NF-YC, RFX5, RFXANK/RFXB, and CREB. We propose that binding of
NF-Y/CBF, RFX, and CREB by CIITA results in a macromolecular complex
which allows transcription factors to interact with the class II MHC
promoter in a spatially and helically constrained fashion.
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Transcriptional Scaffold: CIITA Interacts with
NF-Y, RFX, and CREB To Cause Stereospecific Regulation of the Class II
Major Histocompatibility Complex Promoter

*
Corresponding author. Mailing address: Lineberger
Comprehensive Cancer Center, University of North Carolina at Chapel
Hill, Chapel Hill, NC 27599-7295. Phone: (919) 966-5538. Fax: (919) 966-8212. E-mail: panyun{at}med.unc.edu.
Present address: Howard Hughes Medical Institute, Section of
Immunology, Yale University School of Medicine, New Haven, CT 06510.
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