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Molecular and Cellular Biology, September 2000, p. 6380-6389, Vol. 20, No. 17
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Octamer Transfer and Creation of Stably Remodeled Nucleosomes by Human SWI-SNF and Its Isolated ATPases

Michael L. Phelan,dagger Gavin R. Schnitzler,Dagger and Robert E. Kingston*

Department of Molecular Biology, Massachusetts General Hospital, and Department of Genetics, Harvard Medical School, Boston, Massachusetts

Received 17 March 2000/Returned for modification 1 May 2000/Accepted 23 May 2000

Chromatin remodeling complexes help regulate the structure of chromatin to facilitate transcription. The multisubunit human (h) SWI-SNF complex has been shown to remodel mono- and polynucleosome templates in an ATP-dependent manner. The isolated hSWI-SNF ATPase subunits BRG1 and hBRM also have these activities. The intact complex has been shown to produce a stable remodeled dimer of mononucleosomes as a product. Here we show that the hSWI-SNF ATPases alone can also produce this product. In addition, we show that hSWI-SNF and its ATPases have the ability to transfer histone octamers from donor nucleosomes to acceptor DNA. These two reactions are characterized and compared. Our results are consistent with both products of SWI-SNF action being formed as alternative outcomes of a single remodeling mechanism. The ability of the isolated ATPase subunits to catalyze these reactions suggests that these subunits play a key role in determining the mechanistic capabilities of the SWI-SNF family of remodeling complexes.

* Corresponding author. Mailing address: Dept. of Molecular Biology, Wellman 10, Massachusetts General Hospital, Boston, MA 02114. Phone: (617) 726-5990. Fax: (617) 726-5949. E-mail: kingston{at}frodo.mgh.harvard.edu.

dagger Present address: Zyomyx, Inc., Hayward, CA 94545.

Dagger Present address: Department of Biochemistry, Tufts University School of Medicine, Boston, MA 02111.

Molecular and Cellular Biology, September 2000, p. 6380-6389, Vol. 20, No. 17
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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