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Molecular and Cellular Biology, September 2000, p. 6435-6448, Vol. 20, No. 17
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cell Cycle-Dependent Binding of Yeast Heat Shock Factor to Nucleosomes

Christina Bourgeois Venturi, Alexander M. Erkine, and David S. Gross*

Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130

Received 23 February 2000/Returned for modification 4 April 2000/Accepted 16 May 2000

In the nucleus, transcription factors must contend with the presence of chromatin in order to gain access to their cognate regulatory sequences. As most nuclear DNA is assembled into nucleosomes, activators must either invade a stable, preassembled nucleosome or preempt the formation of nucleosomes on newly replicated DNA, which is transiently free of histones. We have investigated the mechanism by which heat shock factor (HSF) binds to target nucleosomal heat shock elements (HSEs), using as our model a dinucleosomal heat shock promoter (hsp82-Delta HSE1). We find that activated HSF cannot bind a stable, sequence-positioned nucleosome in G1-arrested cells. It can do so readily, however, following release from G1 arrest or after the imposition of either an early S- or late G2-phase arrest. Surprisingly, despite the S-phase requirement, HSF nucleosomal binding activity is restored in the absence of hsp82 replication. These results contrast with the prevailing paradigm for activator-nucleosome interactions and implicate a nonreplicative, S-phase-specific event as a prerequisite for HSF binding to nucleosomal sites in vivo.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130-3932. Phone: (318) 675-5027. Fax: (318) 675-5180. E-mail: dgross{at}lsuhsc.edu.

Molecular and Cellular Biology, September 2000, p. 6435-6448, Vol. 20, No. 17
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kremer, S. B., Gross, D. S. (2009). SAGA and Rpd3 Chromatin Modification Complexes Dynamically Regulate Heat Shock Gene Structure and Expression. J. Biol. Chem. 284: 32914-32931 [Abstract] [Full Text]  
  • Singh, H., Erkine, A. M., Kremer, S. B., Duttweiler, H. M., Davis, D. A., Iqbal, J., Gross, R. R., Gross, D. S. (2006). A Functional Module of Yeast Mediator That Governs the Dynamic Range of Heat-Shock Gene Expression. Genetics 172: 2169-2184 [Abstract] [Full Text]  
  • Zhao, J., Herrera-Diaz, J., Gross, D. S. (2005). Domain-Wide Displacement of Histones by Activated Heat Shock Factor Occurs Independently of Swi/Snf and Is Not Correlated with RNA Polymerase II Density. Mol. Cell. Biol. 25: 8985-8999 [Abstract] [Full Text]  
  • Hahn, J.-S., Hu, Z., Thiele, D. J., Iyer, V. R. (2004). Genome-Wide Analysis of the Biology of Stress Responses through Heat Shock Transcription Factor. Mol. Cell. Biol. 24: 5249-5256 [Abstract] [Full Text]  
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  • Chandrasekharan, M. B., Li, G., Bishop, K. J., Hall, T. C. (2003). S Phase Progression Is Required for Transcriptional Activation of the {beta}-Phaseolin Promoter. J. Biol. Chem. 278: 45397-45405 [Abstract] [Full Text]  


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