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Molecular and Cellular Biology, September 2000, p. 6704-6711, Vol. 20, No. 18
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of the Role of AMP-Activated Protein Kinase in the Regulation of Glucose-Activated Gene Expression Using Constitutively Active and Dominant Negative Forms of the Kinase

Angela Woods,1 Dalila Azzout-Marniche,2 Marc Foretz,2 Silvie C. Stein,1 Patricia Lemarchand,3 Pascal Ferré,2 Fabienne Foufelle,2 and David Carling1,*

Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London W12 0NN, United Kingdom,1 and U465 INSERM, Centre Biomédical des Cordeliers, F-75270 Paris Cedex 06,2 and U25 INSERM, Faculté de Médecine Necker-Enfants Malades, 75730 Paris Cedex 15,3 France

Received 3 April 2000/Returned for modification 19 May 2000/Accepted 20 June 2000

In the liver, glucose induces the expression of a number of genes involved in glucose and lipid metabolism, e.g., those encoding L-type pyruvate kinase and fatty acid synthase. Recent evidence has indicated a role for the AMP-activated protein kinase (AMPK) in the inhibition of glucose-activated gene expression in hepatocytes. It remains unclear, however, whether AMPK is involved in the glucose induction of these genes. In order to study further the role of AMPK in regulating gene expression, we have generated two mutant forms of AMPK. One of these (alpha 1312) acts as a constitutively active kinase, while the other (alpha 1DN) acts as a dominant negative inhibitor of endogenous AMPK. We have used adenovirus-mediated gene transfer to express these mutants in primary rat hepatocytes in culture in order to determine their effect on AMPK activity and the transcription of glucose-activated genes. Expression of alpha 1312 increased AMPK activity in hepatocytes and blocked completely the induction of a number of glucose-activated genes in response to 25 mM glucose. This effect is similar to that observed following activation of AMPK by 5-amino-imidazolecarboxamide riboside. Expression of alpha 1DN markedly inhibited both basal and stimulated activity of endogenous AMPK but had no effect on the transcription of glucose-activated genes. Our results suggest that AMPK is involved in the inhibition of glucose-activated gene expression but not in the induction pathway. This study demonstrates that the two mutants we have described will provide valuable tools for studying the wider physiological role of AMPK.

* Corresponding author. Mailing address: Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, DuCane Road, London W12 0NN, United Kingdom. Phone: 44 (0)20 8383 4313. Fax: 44 (0)20 8383 2028. E-mail: dcarling{at}csc.mrc.ac.uk.

Molecular and Cellular Biology, September 2000, p. 6704-6711, Vol. 20, No. 18
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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