A Human Condensin Complex Containing hCAP-C-hCAP-E and CNAP1, a Homolog of Xenopus XCAP-D2, Colocalizes with Phosphorylated Histone H3 during the Early Stage of Mitotic Chromosome Condensation

doi:10.1128/MCB.20.18.6996-7006.2000

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Schmiesing, J. A.
	Articles by Yokomori, K.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Schmiesing, J. A.
	Articles by Yokomori, K.

Previous Article | Next Article

Molecular and Cellular Biology, September 2000, p. 6996-7006, Vol. 20, No. 18
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Human Condensin Complex Containing hCAP-C-hCAP-E and CNAP1, a Homolog of Xenopus XCAP-D2, Colocalizes with Phosphorylated Histone H3 during the Early Stage of Mitotic Chromosome Condensation

John A. Schmiesing,¹ Heather C. Gregson,¹ Sharleen Zhou,² and Kyoko Yokomori¹^,^*

Department of Biological Chemistry, College of Medicine, University of California, Irvine, California 92697-1700,¹ and Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3202²

Received 11 January 2000/Returned for modification 9 February 2000/Accepted 15 June 2000

Structural maintenance of chromosomes (SMC) family proteins play critical roles in structural changes of chromosomes. Previously,we identified two human SMC family proteins, hCAP-C and hCAP-E,which form a heterodimeric complex (hCAP-C-hCAP-E) in the cell.Based on the sequence conservation and mitotic chromosome localization,hCAP-C-hCAP-E was determined to be the human ortholog of the XenopusSMC complex, XCAP-C-XCAP-E. XCAP-C-XCAP-E is a component of themultiprotein complex termed condensin, required for mitotic chromosomecondensation in vitro. However, presence of such a complex hasnot been demonstrated in mammalian cells. Coimmunoprecipitationof the endogenous hCAP-C-hCAP-E complex from HeLa extracts identifieda 155-kDa protein interacting with hCAP-C-hCAP-E, termed condensation-relatedSMC-associated protein 1 (CNAP1). CNAP1 associates with mitoticchromosomes and is homologous to Xenopus condensin component XCAP-D2,indicating the presence of a condensin complex in human cells.Chromosome association of human condensin is mitosis specific,and the majority of condensin dissociates from chromosomes andis sequestered in the cytoplasm throughout interphase. However,a subpopulation of the complex was found to remain on chromosomesas foci in the interphase nucleus. During late G₂/early prophase,the larger nuclear condensin foci colocalize with phosphorylatedhistone H3 clusters on partially condensed regions of chromosomes.These results suggest that mitosis-specific function of humancondensin may be regulated by cell cycle-specific subcellularlocalization of the complex, and the nuclear condensin that associateswith interphase chromosomes is involved in the reinitiation ofmitotic chromosome condensation in conjunction with phosphorylationof histoneH3.

^* Corresponding author. Mailing address: 240D Med Sci I, Department of Biological Chemistry, College of Medicine, Universityof California, Irvine, CA 92697-1700. Phone: (949) 824-8215. Fax:(949) 824-2688. E-mail: kyokomor{at}uci.edu.

Molecular and Cellular Biology, September 2000, p. 6996-7006, Vol. 20, No. 18
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

Swain, J. E., Ding, J., Wu, J., Smith, G. D. (2008). Regulation of spindle and chromatin dynamics during early and late stages of oocyte maturation by aurora kinases. Mol Hum Reprod 14: 291-299 [Abstract] [Full Text]
Takemoto, A., Murayama, A., Katano, M., Urano, T., Furukawa, K., Yokoyama, S., Yanagisawa, J., Hanaoka, F., Kimura, K. (2007). Analysis of the role of Aurora B on the chromosomal targeting of condensin I. Nucleic Acids Res 35: 2403-2412 [Abstract] [Full Text]
Swain, J. E., Ding, J., Brautigan, D. L., Villa-Moruzzi, E., Smith, G. D. (2007). Proper Chromatin Condensation and Maintenance of Histone H3 Phosphorylation During Mouse Oocyte Meiosis Requires Protein Phosphatase Activity. Biol. Reprod. 76: 628-638 [Abstract] [Full Text]
Lam, W. W., Peterson, E. A., Yeung, M., Lavoie, B. D. (2006). Condensin is required for chromosome arm cohesion during mitosis.. Genes Dev. 20: 2973-2984 [Abstract] [Full Text]
Blank, M., Lerenthal, Y., Mittelman, L., Shiloh, Y. (2006). Condensin I recruitment and uneven chromatin condensation precede mitotic cell death in response to DNA damage. J. Cell Biol. 174: 195-206 [Abstract] [Full Text]
Jelinkova, L., Kubelka, M. (2006). Neither Aurora B Activity nor Histone H3 Phosphorylation Is Essential for Chromosome Condensation During Meiotic Maturation of Porcine Oocytes. Biol. Reprod. 74: 905-912 [Abstract] [Full Text]
Staudt, N., Fellert, S., Chung, H.-R., Jackle, H., Vorbruggen, G. (2006). Mutations of the Drosophila Zinc Finger-encoding Gene vielfaltig Impair Mitotic Cell Divisions and Cause Improper Chromosome Segregation. Mol. Biol. Cell 17: 2356-2365 [Abstract] [Full Text]
Lam, W. S., Yang, X., Makaroff, C. A. (2005). Characterization of Arabidopsis thaliana SMC1 and SMC3: evidence that AtSMC3 may function beyond chromosome cohesion. J. Cell Sci. 118: 3037-3048 [Abstract] [Full Text]
Savvidou, E., Cobbe, N., Steffensen, S., Cotterill, S., Heck, M. M. S. (2005). Drosophila CAP-D2 is required for condensin complex stability and resolution of sister chromatids. J. Cell Sci. 118: 2529-2543 [Abstract] [Full Text]
Haumaitre, C., Barbacci, E., Jenny, M., Ott, M. O., Gradwohl, G., Cereghini, S. (2005). Lack of TCF2/vHNF1 in mice leads to pancreas agenesis. Proc. Natl. Acad. Sci. USA 102: 1490-1495 [Abstract] [Full Text]
Watrin, E., Legagneux, V. (2005). Contribution of hCAP-D2, a Non-SMC Subunit of Condensin I, to Chromosome and Chromosomal Protein Dynamics during Mitosis. Mol. Cell. Biol. 25: 740-750 [Abstract] [Full Text]
Hirota, T., Gerlich, D., Koch, B., Ellenberg, J., Peters, J.-M. (2004). Distinct functions of condensin I and II in mitotic chromosome assembly. J. Cell Sci. 117: 6435-6445 [Abstract] [Full Text]
Ono, T., Fang, Y., Spector, D. L., Hirano, T. (2004). Spatial and Temporal Regulation of Condensins I and II in Mitotic Chromosome Assembly in Human Cells. Mol. Biol. Cell 15: 3296-3308 [Abstract] [Full Text]
Geiman, T. M., Sankpal, U. T., Robertson, A. K., Chen, Y., Mazumdar, M., Heale, J. T., Schmiesing, J. A., Kim, W., Yokomori, K., Zhao, Y., Robertson, K. D. (2004). Isolation and characterization of a novel DNA methyltransferase complex linking DNMT3B with components of the mitotic chromosome condensation machinery. Nucleic Acids Res 32: 2716-2729 [Abstract] [Full Text]
Bort, R., Martinez-Barbera, J. P., Beddington, R. S. P., Zaret, K. S. (2004). Hex homeobox gene-dependent tissue positioning is required for organogenesis of the ventral pancreas. Development 131: 797-806 [Abstract] [Full Text]
Takemoto, A., Kimura, K., Yokoyama, S., Hanaoka, F. (2004). Cell Cycle-dependent Phosphorylation, Nuclear Localization, and Activation of Human Condensin. J. Biol. Chem. 279: 4551-4559 [Abstract] [Full Text]
Galy, V., Mattaj, I. W., Askjaer, P. (2003). Caenorhabditis elegans Nucleoporins Nup93 and Nup205 Determine the Limit of Nuclear Pore Complex Size Exclusion In Vivo. Mol. Biol. Cell 14: 5104-5115 [Abstract] [Full Text]
Cimini, D., Mattiuzzo, M., Torosantucci, L., Degrassi, F. (2003). Histone Hyperacetylation in Mitosis Prevents Sister Chromatid Separation and Produces Chromosome Segregation Defects. Mol. Biol. Cell 14: 3821-3833 [Abstract] [Full Text]
Uzbekov, R., Timirbulatova, E., Watrin, E., Cubizolles, F., Ogereau, D., Gulak, P., Legagneux, V., Polyakov, V. Ju., Le Guellec, K., Kireev, I. (2003). Nucleolar association of pEg7 and XCAP-E, two members of Xenopus laevis condensin complex in interphase cells. J. Cell Sci. 116: 1667-1678 [Abstract] [Full Text]
Przewloka, M. R., Pardington, P. E., Yannone, S. M., Chen, D. J., Cary, R. B. (2003). In Vitro and In Vivo Interactions of DNA Ligase IV with a Subunit of the Condensin Complex. Mol. Biol. Cell 14: 685-697 [Abstract] [Full Text]
Losada, A., Hirano, M., Hirano, T. (2002). Cohesin release is required for sister chromatid resolution, but not for condensin-mediated compaction, at the onset of mitosis. Genes Dev. 16: 3004-3016 [Abstract] [Full Text]
Kim, J.-S., Krasieva, T. B., LaMorte, V., Taylor, A. M. R., Yokomori, K. (2002). Specific Recruitment of Human Cohesin to Laser-induced DNA Damage. J. Biol. Chem. 277: 45149-45153 [Abstract] [Full Text]
Ball, A. R. Jr., Schmiesing, J. A., Zhou, C., Gregson, H. C., Okada, Y., Doi, T., Yokomori, K. (2002). Identification of a Chromosome-Targeting Domain in the Human Condensin Subunit CNAP1/hCAP-D2/Eg7. Mol. Cell. Biol. 22: 5769-5781 [Abstract] [Full Text]
Bomar, J., Moreira, P., Balise, J. J., Collas, P. (2002). Differential regulation of maternal and paternal chromosome condensation in mitotic zygotes. J. Cell Sci. 115: 2931-2940 [Abstract] [Full Text]
Stear, J. H., Roth, M. B. (2002). Characterization of HCP-6, a C. elegans protein required to prevent chromosome twisting and merotelic attachment. Genes Dev. 16: 1498-1508 [Abstract] [Full Text]
Hagstrom, K. A., Holmes, V. F., Cozzarelli, N. R., Meyer, B. J. (2002). C. elegans condensin promotes mitotic chromosome architecture, centromere organization, and sister chromatid segregation during mitosis and meiosis. Genes Dev. 16: 729-742 [Abstract] [Full Text]
Petersen, J., Paris, J., Willer, M., Philippe, M., Hagan, I. M. (2002). The S. pombe aurora-related kinase Ark1 associates with mitotic structures in a stage dependent manner and is required for chromosome segregation. J. Cell Sci. 114: 4371-4384 [Abstract] [Full Text]
Hirano, T. (2002). The ABCs of SMC proteins: two-armed ATPases for chromosome condensation, cohesion, and repair. Genes Dev. 16: 399-414 [Full Text]
Gregson, H. C., Schmiesing, J. A., Kim, J.-S., Kobayashi, T., Zhou, S., Yokomori, K. (2001). A Potential Role for Human Cohesin in Mitotic Spindle Aster Assembly. J. Biol. Chem. 276: 47575-47582 [Abstract] [Full Text]
Cabello, O. A., Eliseeva, E., He, W., Youssoufian, H., Plon, S. E., Brinkley, B. R., Belmont, J. W. (2001). Cell Cycle-dependent Expression and Nucleolar Localization of hCAP-H. Mol. Biol. Cell 12: 3527-3537 [Abstract] [Full Text]
Giet, R., Glover, D. M. (2001). Drosophila Aurora B Kinase Is Required for Histone H3 Phosphorylation and Condensin Recruitment during Chromosome Condensation and to Organize the Central Spindle during Cytokinesis. J. Cell Biol. 152: 669-682 [Abstract] [Full Text]
Kimura, K., Cuvier, O., Hirano, T. (2001). Chromosome Condensation by a Human Condensin Complex in Xenopus Egg Extracts. J. Biol. Chem. 276: 5417-5420 [Abstract] [Full Text]
Kruhlak, M. J., Hendzel, M. J., Fischle, W., Bertos, N. R., Hameed, S., Yang, X.-J., Verdin, E., Bazett-Jones, D. P. (2001). Regulation of Global Acetylation in Mitosis through Loss of Histone Acetyltransferases and Deacetylases from Chromatin. J. Biol. Chem. 276: 38307-38319 [Abstract] [Full Text]
Zeitlin, S. G., Shelby, R. D., Sullivan, K. F. (2001). CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis. J. Cell Biol. 155: 1147-1158 [Abstract] [Full Text]

J. Bacteriol.	J. Virol.	Eukaryot. Cell

Microbiol. Mol. Biol. Rev.	Clin. Vaccine Immunol.	All ASM Journals