Transcriptional Regulation of the CLC-K1 Promoter by myc-Associated Zinc Finger Protein and Kidney-Enriched Kruppel-Like Factor, a Novel Zinc Finger Repressor

doi:10.1128/MCB.20.19.7319-7331.2000

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Uchida, S.
	Articles by Marumo, F.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Uchida, S.
	Articles by Marumo, F.

Previous Article | Next Article

Molecular and Cellular Biology, October 2000, p. 7319-7331, Vol. 20, No. 19
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Transcriptional Regulation of the CLC-K1 Promoter by myc-Associated Zinc Finger Protein and Kidney-Enriched Krüppel-Like Factor, a Novel Zinc Finger Repressor

Shinichi Uchida,¹^,^* Yujiro Tanaka,¹ Hiroshi Ito,¹ Fumiko Saitoh-Ohara,² Johji Inazawa,² Kazunari K. Yokoyama,³ Sei Sasaki,¹ and Fumiaki Marumo¹

Second Department of Internal Medicine, School of Medicine,¹ and Department of Molecular Cytogenetics, Medical Research Institute,² Tokyo Medical and Dental University, Tokyo, and Tukuba Institute, RIKEN (The Institute of Physical and Chemical Research), Ibaraki,³ Japan

Received 15 May 2000/Accepted 21 June 2000

The expression of CLC-K1 and CLC-K2, two kidney-specific CLC chloride channels, is transcriptionally regulated on a tissue-specificbasis. Previous studies have shown that a GA element near theirtranscriptional start sites is important for basal and cell-specificactivities of the CLC-K1 and CLC-K2 gene promoters. To identifythe GA-binding proteins, the human kidney cDNA library was screenedby a yeast one-hybrid system. A novel member of the Cys2-His2zinc finger gene designated KKLF (for "kidney-enriched Krüppel-likefactor") and the previously isolated MAZ (for "myc-associatedzinc finger protein") were cloned. KKLF was found to be abundantlyexpressed in the liver, kidneys, heart, and skeletal muscle, andimmunohistochemistry revealed the nuclear localization of KKLFprotein in interstitial cells in heart and skeletal muscle, stellatecells, and fibroblasts in the liver. In the kidneys, KKLF proteinwas localized in interstitial cells, mesangial cells, and nephronsegments, where CLC-K1 and CLC-K2 were not expressed. A gel mobilityshift assay revealed sequence-specific binding of recombinantKKLF and MAZ proteins to the CLC-K1 GA element, and the fine-mutationassay clarified that the consensus sequence for the KKLF bindingsite was GGGGNGGNG. In a transient-transfection experiment, MAZhad a strong activating effect on transcription of the CLC-K1-luciferasereporter gene. On the other hand, KKLF coexpression with MAZ appearedto block the activating effect of MAZ. These results suggest thata novel set of zinc finger proteins may help regulate the stricttissue- and nephron segment-specific expression of the CLC-K1and CLC-K2 channel genes through their GA ciselement.

^* Corresponding author. Mailing address: Second Department of Internal Medicine, School of Medicine, Tokyo Medical and DentalUniversity, 1-5-45 Yushima Bunkyo, Tokyo 113-8519, Japan. Phone:81-3-5803-5216. Fax: 81-3-5803-0172. e-mail: suchida.med2{at}med.tmd.ac.jp.

Molecular and Cellular Biology, October 2000, p. 7319-7331, Vol. 20, No. 19
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

Adler, L., Efrati, E., Zelikovic, I. (2008). Molecular mechanisms of epithelial cell-specific expression and regulation of the human anion exchanger (pendrin) gene. Am. J. Physiol. Cell Physiol. 294: C1261-C1276 [Abstract] [Full Text]
Natesampillai, S., Kerkvliet, J., Leung, P. C. K., Veldhuis, J. D. (2008). Regulation of Kruppel-like factor 4, 9, and 13 genes and the steroidogenic genes LDLR, StAR, and CYP11A in ovarian granulosa cells. Am. J. Physiol. Endocrinol. Metab. 294: E385-E391 [Abstract] [Full Text]
Fisch, S., Gray, S., Heymans, S., Haldar, S. M., Wang, B., Pfister, O., Cui, L., Kumar, A., Lin, Z., Sen-Banerjee, S., Das, H., Petersen, C. A., Mende, U., Burleigh, B. A., Zhu, Y., Pinto, Y. M., Liao, R., Jain, M. K. (2007). Kruppel-like factor 15 is a regulator of cardiomyocyte hypertrophy. Proc. Natl. Acad. Sci. USA 104: 7074-7079 [Abstract] [Full Text]
Malakooti, J., Sandoval, R., Memark, V. C., Dudeja, P. K., Ramaswamy, K. (2005). Zinc finger transcription factor Egr-1 is involved in stimulation of NHE2 gene expression by phorbol 12-myristate 13-acetate. Am. J. Physiol. Gastrointest. Liver Physiol. 289: G653-G663 [Abstract] [Full Text]
Mori, T., Sakaue, H., Iguchi, H., Gomi, H., Okada, Y., Takashima, Y., Nakamura, K., Nakamura, T., Yamauchi, T., Kubota, N., Kadowaki, T., Matsuki, Y., Ogawa, W., Hiramatsu, R., Kasuga, M. (2005). Role of Kruppel-like Factor 15 (KLF15) in Transcriptional Regulation of Adipogenesis. J. Biol. Chem. 280: 12867-12875 [Abstract] [Full Text]
Efrati, E., Arsentiev-Rozenfeld, J., Zelikovic, I. (2005). The human paracellin-1 gene (hPCLN-1): renal epithelial cell-specific expression and regulation. Am. J. Physiol. Renal Physiol. 288: F272-F283 [Abstract] [Full Text]
Ray, A., Kumar, D., Ray, P., Ray, B. K. (2004). Transcriptional Activity of Serum Amyloid A-activating Factor-1 Is Regulated by Distinct Functional Modules. J. Biol. Chem. 279: 54637-54646 [Abstract] [Full Text]
Otteson, D. C., Liu, Y., Lai, H., Wang, C., Gray, S., Jain, M. K., Zack, D. J. (2004). Kruppel-like Factor 15, a Zinc-Finger Transcriptional Regulator, Represses the Rhodopsin and Interphotoreceptor Retinoid-Binding Protein Promoters. IOVS 45: 2522-2530 [Abstract] [Full Text]
Yamamoto, J., Ikeda, Y., Iguchi, H., Fujino, T., Tanaka, T., Asaba, H., Iwasaki, S., Ioka, R. X., Kaneko, I. W., Magoori, K., Takahashi, S., Mori, T., Sakaue, H., Kodama, T., Yanagisawa, M., Yamamoto, T. T., Ito, S., Sakai, J. (2004). A Kruppel-like factor KLF15 Contributes Fasting-induced Transcriptional Activation of Mitochondrial Acetyl-CoA Synthetase Gene AceCS2. J. Biol. Chem. 279: 16954-16962 [Abstract] [Full Text]
Ray, A., Ray, P., Guthrie, N., Shakya, A., Kumar, D., Ray, B. K. (2003). Protein Kinase A Signaling Pathway Regulates Transcriptional Activity of SAF-1 by Unmasking Its DNA-binding Domains. J. Biol. Chem. 278: 22586-22595 [Abstract] [Full Text]
Auzanneau, C., Thoreau, V., Kitzis, A., Becq, F. (2003). A Novel Voltage-dependent Chloride Current Activated by Extracellular Acidic pH in Cultured Rat Sertoli Cells. J. Biol. Chem. 278: 19230-19236 [Abstract] [Full Text]
Ray, B. K., Murphy, R., Ray, P., Ray, A. (2002). SAF-2, a Splice Variant of SAF-1, Acts as a Negative Regulator of Transcription. J. Biol. Chem. 277: 46822-46830 [Abstract] [Full Text]
Gray, S., Feinberg, M. W., Hull, S., Kuo, C. T., Watanabe, M., Sen-Banerjee, S., DePina, A., Haspel, R., Jain, M. K. (2002). The Kruppel-like Factor KLF15 Regulates the Insulin-sensitive Glucose Transporter GLUT4. J. Biol. Chem. 277: 34322-34328 [Abstract] [Full Text]
Higaki, Y., Schullery, D., Kawata, Y., Shnyreva, M., Abrass, C., Bomsztyk, K. (2002). Synergistic activation of the rat laminin {gamma}1 chain promoter by the gut-enriched Kruppel-like factor (GKLF/KLF4) and Sp1. Nucleic Acids Res 30: 2270-2279 [Abstract] [Full Text]
Yoshida, T., Yoshino, J., Hayashi, M., Saruta, T. (2002). Identification of a Renal Proximal Tubular Cell-Specific Enhancer in the Mouse 25-Hydroxyvitamin D 1{alpha}-Hydroxylase Gene. J. Am. Soc. Nephrol. 13: 1455-1463 [Abstract] [Full Text]
Jentsch, T. J., Stein, V., Weinreich, F., Zdebik, A. A. (2002). Molecular Structure and Physiological Function of Chloride Channels. Physiol. Rev. 82: 503-568 [Abstract] [Full Text]
Zhang, F., Nakanishi, G., Kurebayashi, S., Yoshino, K., Perantoni, A., Kim, Y.-S., Jetten, A. M. (2002). Characterization of Glis2, a Novel Gene Encoding a Gli-related, Kruppel-like Transcription Factor with Transactivation and Repressor Functions. ROLES IN KIDNEY DEVELOPMENT AND NEUROGENESIS. J. Biol. Chem. 277: 10139-10149 [Abstract] [Full Text]
Ray, A., Yu, G.-Y., Ray, B. K. (2002). Cytokine-Responsive Induction of SAF-1 Activity Is Mediated by a Mitogen-Activated Protein Kinase Signaling Pathway. Mol. Cell. Biol. 22: 1027-1035 [Abstract] [Full Text]
Ray, B. K., Chen, J., Ray, A. (2001). Catalytic Subunit of Protein Kinase A Is an Interacting Partner of the Inflammation-Responsive Transcription Factor Serum Amyloid A-Activating Factor-1. J. Immunol. 167: 2343-2348 [Abstract] [Full Text]
Lei, L, Ma, L, Nef, S, Thai, T, Parada, L. (2001). mKlf7, a potential transcriptional regulator of TrkA nerve growth factor receptor expression in sensory and sympathetic neurons. Development 128: 1147-1158 [Abstract]
Albert, T., Wells, J., Funk, J.-O., Pullner, A., Raschke, E.-E., Stelzer, G., Meisterernst, M., Farnham, P. J., Eick, D. (2001). The Chromatin Structure of the Dual c-myc Promoter P1/P2 Is Regulated by Separate Elements. J. Biol. Chem. 276: 20482-20490 [Abstract] [Full Text]
Taniyama, Y., Sato, K., Sugawara, A., Uruno, A., Ikeda, Y., Kudo, M., Ito, S., Takeuchi, K. (2001). Renal Tubule-specific Transcription and Chromosomal Localization of Rat Thiazide-sensitive Na-Cl Cotransporter Gene. J. Biol. Chem. 276: 26260-26268 [Abstract] [Full Text]
Bieker, J. J. (2001). Kruppel-like Factors: Three Fingers in Many Pies. J. Biol. Chem. 276: 34355-34358 [Full Text]

J. Bacteriol.	J. Virol.	Eukaryot. Cell

Microbiol. Mol. Biol. Rev.	Clin. Vaccine Immunol.	All ASM Journals