A Novel Subunit of Yeast RNA Polymerase III Interacts with the TFIIB-Related Domain of TFIIIB70

doi:10.1128/MCB.20.2.488-495.2000

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Molecular and Cellular Biology, January 2000, p. 488-495, Vol. 20, No. 2
0270-7306/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Novel Subunit of Yeast RNA Polymerase III Interacts with the TFIIB-Related Domain of TFIIIB70

Maria-Laura Ferri, Gérald Peyroche, Magali Siaut, Olivier Lefebvre, Christophe Carles, Christine Conesa, and André Sentenac^*

Service de Biochimie et de Génétique Moléculaire, CEA/Saclay, F-91191 Gif-sur-Yvette Cedex, France

Received 9 July 1999/Returned for modification 2 September 1999/Accepted 13 October 1999

There is limited information on how eukaryotic RNA polymerases (Pol) recognize their cognate preinitiation complex. We havecharacterized a polypeptide copurifying with yeast Pol III. Thisprotein, C17, was found to be homologous to a mammalian proteindescribed as a hormone receptor. Deletion of the correspondinggene, RPC17, was lethal and its regulated extinction caused aselective defect in transcription of class III genes in vivo.Two-hybrid and coimmunoprecipitation experiments indicated thatC17 interacts with two Pol III subunits, one of which, C31, isimportant for the initiation reaction. C17 also interacted withTFIIIB70, the TFIIB-related component of TFIIIB. The interactiondomain was found to be in the N-terminal, TFIIB-like half of TFIIIB70,downstream of the zinc ribbon and first imperfect repeat. AlthoughPol II similarly interacts with TFIIB, it is notable that C17has no similarity to any Pol II subunit. The data indicate thatC17 is a novel specific subunit of Pol III which participatestogether with C34 in the recruitment of Pol III by the preinitiationcomplex.

^* Corresponding author. Mailing address: Service de Biochimie et de Génétique Moléculaire, CEA/Saclay, F-91191 Gif-sur-YvetteCedex, France. Phone: 33 1 69 08 22 36. Fax: 33 1 69 08 47 12.E-mail: sentenac{at}dsvidf.cea.fr.

Present address: Génétique des Interactions Macromoléculaires, CNRS (URA 1300), Institut Pasteur, 75724 Paris Cedex 15,France.

Molecular and Cellular Biology, January 2000, p. 488-495, Vol. 20, No. 2
0270-7306/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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