Inhibition of TATA-Binding Protein Function by SAGA Subunits Spt3 and Spt8 at Gcn4-Activated Promoters

doi:10.1128/MCB.20.2.634-647.2000

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Molecular and Cellular Biology, January 2000, p. 634-647, Vol. 20, No. 2
0270-7306/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Inhibition of TATA-Binding Protein Function by SAGA Subunits Spt3 and Spt8 at Gcn4-Activated Promoters

Rimma Belotserkovskaya,¹ David E. Sterner,¹ Min Deng,² Michael H. Sayre,² Paul M. Lieberman,¹ and Shelley L. Berger¹^,^*

The Wistar Institute, Philadelphia, Pennsylvania 19104,¹ and Johns Hopkins University, Baltimore, Maryland 21205²

Received 3 August 1999/Returned for modification 5 October 1999/Accepted 13 October 1999

SAGA is a 1.8-MDa yeast protein complex that is composed of several distinct classes of transcription-related factors, includingthe adaptor/acetyltransferase Gcn5, Spt proteins, and a subsetof TBP-associated factors. Our results indicate that mutationsthat completely disrupt SAGA (deletions of SPT7 or SPT20) stronglyreduce transcriptional activation at the HIS3 and TRP3 genes andthat Gcn5 is required for normal HIS3 transcriptional start siteselection. Surprisingly, mutations in Spt proteins involved inthe SAGA-TBP interaction (Spt3 and Spt8) cause derepression ofHIS3 and TRP3 transcription in the uninduced state. Consistentwith this finding, wild-type SAGA inhibits TBP binding to theHIS3 promoter in vitro, while SAGA lacking Spt3 or Spt8 is notinhibitory. We detected two distinct forms of SAGA in cell extractsand, strikingly, one lacks Spt8. Conditions that induce HIS3 andTRP3 transcription result in an altered balance between thesecomplexes strongly in favor of the form without Spt8. These resultssuggest that the composition of SAGA may be dynamic in vivo andmay be regulated through dissociable inhibitorysubunits.

^* Corresponding author. Mailing address: The Wistar Institute, 3601 Spruce St., Room 389, Philadelphia, PA 19104. Phone: (215)898-3922. Fax: (215) 898-0663. E-mail: berger{at}wista.wistar.upenn.edu.

Molecular and Cellular Biology, January 2000, p. 634-647, Vol. 20, No. 2
0270-7306/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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