This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kobor, M. S.
Right arrow Articles by Greenblatt, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kobor, M. S.
Right arrow Articles by Greenblatt, J.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2000, p. 7438-7449, Vol. 20, No. 20
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Motif Shared by TFIIF and TFIIB Mediates Their Interaction with the RNA Polymerase II Carboxy-Terminal Domain Phosphatase Fcp1p in Saccharomyces cerevisiae

Michael S. Kobor,1,2 Lisa D. Simon,1 Jim Omichinski,1,3 Guoqing Zhong,1 Jacques Archambault,4 and Jack Greenblatt1,2,*

Banting and Best Department of Medical Research1 and Department of Molecular and Medical Genetics,2 University of Toronto, Toronto, Ontario M5G 1L6, and Department of Biological Sciences, Boehringer Ingelheim (Canada) Limited, Laval, Quebec H7S 2G5,4 Canada, and Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 306023

Received 13 June 2000/Returned for modification 16 July 2000/Accepted 31 July 2000

Transcription by RNA polymerase II is accompanied by cyclic phosphorylation and dephosphorylation of the carboxy-terminal heptapeptide repeat domain (CTD) of its largest subunit. We have used deletion and point mutations in Fcp1p, a TFIIF-interacting CTD phosphatase, to show that the integrity of its BRCT domain, like that of its catalytic domain, is important for cell viability, mRNA synthesis, and CTD dephosphorylation in vivo. Although regions of Fcp1p carboxy terminal to its BRCT domain and at its amino terminus were not essential for viability, deletion of either of these regions affected the phosphorylation state of the CTD. Two portions of this carboxy-terminal region of Fcp1p bound directly to the first cyclin-like repeat in the core domain of the general transcription factor TFIIB, as well as to the RAP74 subunit of TFIIF. These regulatory interactions with Fcp1p involved closely related amino acid sequence motifs in TFIIB and RAP74. Mutating the Fcp1p-binding motif KEFGK in the RAP74 (Tfg1p) subunit of TFIIF to EEFGE led to both synthetic phenotypes in certain fcp1 tfg1 double mutants and a reduced ability of Fcp1p to activate transcription when it is artificially tethered to a promoter. These results suggest strongly that this KEFGK motif in RAP74 mediates its interaction with Fcp1p in vivo.

* Corresponding author. Mailing address: Banting and Best Department of Medical Research, University of Toronto, 112 College St., Rm. 210, Toronto, Ontario, Canada M5G 1L6. Phone: (416) 978-4141. Fax: (416) 978-8528. E-mail: jack.greenblatt{at}utoronto.ca.

Molecular and Cellular Biology, October 2000, p. 7438-7449, Vol. 20, No. 20
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ohrmalm, C., Akusjarvi, G. (2006). Cellular Splicing and Transcription Regulatory Protein p32 Represses Adenovirus Major Late Transcription and Causes Hyperphosphorylation of RNA Polymerase II.. J. Virol. 80: 5010-5020 [Abstract] [Full Text]  
  • Yang, P. K., Hoareau, C., Froment, C., Monsarrat, B., Henry, Y., Chanfreau, G. (2005). Cotranscriptional Recruitment of the Pseudouridylsynthetase Cbf5p and of the RNA Binding Protein Naf1p during H/ACA snoRNP Assembly. Mol. Cell. Biol. 25: 3295-3304 [Abstract] [Full Text]  
  • Kong, S. E., Kobor, M. S., Krogan, N. J., Somesh, B. P., Sogaard, T. M. M., Greenblatt, J. F., Svejstrup, J. Q. (2005). Interaction of Fcp1 Phosphatase with Elongating RNA Polymerase II Holoenzyme, Enzymatic Mechanism of Action, and Genetic Interaction with Elongator. J. Biol. Chem. 280: 4299-4306 [Abstract] [Full Text]  
  • Ghazy, M. A., Brodie, S. A., Ammerman, M. L., Ziegler, L. M., Ponticelli, A. S. (2004). Amino Acid Substitutions in Yeast TFIIF Confer Upstream Shifts in Transcription Initiation and Altered Interaction with RNA Polymerase II. Mol. Cell. Biol. 24: 10975-10985 [Abstract] [Full Text]  
  • Jeronimo, C., Langelier, M.-F., Zeghouf, M., Cojocaru, M., Bergeron, D., Baali, D., Forget, D., Mnaimneh, S., Davierwala, A. P., Pootoolal, J., Chandy, M., Canadien, V., Beattie, B. K., Richards, D. P., Workman, J. L., Hughes, T. R., Greenblatt, J., Coulombe, B. (2004). RPAP1, a Novel Human RNA Polymerase II-Associated Protein Affinity Purified with Recombinant Wild-Type and Mutated Polymerase Subunits. Mol. Cell. Biol. 24: 7043-7058 [Abstract] [Full Text]  
  • Fath, S., Kobor, M. S., Philippi, A., Greenblatt, J., Tschochner, H. (2004). Dephosphorylation of RNA Polymerase I by Fcp1p Is Required for Efficient rRNA Synthesis. J. Biol. Chem. 279: 25251-25259 [Abstract] [Full Text]  
  • Hausmann, S., Erdjument-Bromage, H., Shuman, S. (2004). Schizosaccharomyces pombe Carboxyl-terminal Domain (CTD) Phosphatase Fcp1: DISTRIBUTIVE MECHANISM, MINIMAL CTD SUBSTRATE, AND ACTIVE SITE MAPPING. J. Biol. Chem. 279: 10892-10900 [Abstract] [Full Text]  
  • Yeo, M., Lin, P. S., Dahmus, M. E., Gill, G. N. (2003). A Novel RNA Polymerase II C-terminal Domain Phosphatase That Preferentially Dephosphorylates Serine 5. J. Biol. Chem. 278: 26078-26085 [Abstract] [Full Text]  
  • Nguyen, B. D., Abbott, K. L., Potempa, K., Kobor, M. S., Archambault, J., Greenblatt, J., Legault, P., Omichinski, J. G. (2003). NMR structure of a complex containing the TFIIF subunit RAP74 and the RNA polymerase II carboxyl-terminal domain phosphatase FCP1. Proc. Natl. Acad. Sci. USA 100: 5688-5693 [Abstract] [Full Text]  
  • Hausmann, S., Shuman, S. (2003). Defining the Active Site of Schizosaccharomyces pombe C-terminal Domain Phosphatase Fcp1. J. Biol. Chem. 278: 13627-13632 [Abstract] [Full Text]  
  • Kamada, K., Roeder, R. G., Burley, S. K. (2003). Molecular mechanism of recruitment of TFIIF- associating RNA polymerase C-terminal domain phosphatase (FCP1) by transcription factor IIF. Proc. Natl. Acad. Sci. USA 100: 2296-2299 [Abstract] [Full Text]  
  • Friedl, E. M., Lane, W. S., Erdjument-Bromage, H., Tempst, P., Reinberg, D. (2003). The C-terminal domain phosphatase and transcription elongation activities of FCP1 are regulated by phosphorylation. Proc. Natl. Acad. Sci. USA 100: 2328-2333 [Abstract] [Full Text]  
  • Licciardo, P., Amente, S., Ruggiero, L., Monti, M., Pucci, P., Lania, L., Majello, B. (2003). The FCP1 phosphatase interacts with RNA polymerase II and with MEP50 a component of the methylosome complex involved in the assembly of snRNP. Nucleic Acids Res 31: 999-1005 [Abstract] [Full Text]  
  • Mandal, S. S., Cho, H., Kim, S., Cabane, K., Reinberg, D. (2002). FCP1, a Phosphatase Specific for the Heptapeptide Repeat of the Largest Subunit of RNA Polymerase II, Stimulates Transcription Elongation. Mol. Cell. Biol. 22: 7543-7552 [Abstract] [Full Text]  
  • Palancade, B., Dubois, M.-F., Bensaude, O. (2002). FCP1 Phosphorylation by Casein Kinase 2 Enhances Binding to TFIIF and RNA Polymerase II Carboxyl-terminal Domain Phosphatase Activity. J. Biol. Chem. 277: 36061-36067 [Abstract] [Full Text]  
  • Hausmann, S., Shuman, S. (2002). Characterization of the CTD Phosphatase Fcp1 from Fission Yeast. PREFERENTIAL DEPHOSPHORYLATION OF SERINE 2 VERSUS SERINE 5. J. Biol. Chem. 277: 21213-21220 [Abstract] [Full Text]  
  • Kimura, M., Suzuki, H., Ishihama, A. (2002). Formation of a Carboxy-Terminal Domain Phosphatase (Fcp1)/TFIIF/RNA Polymerase II (pol II) Complex in Schizosaccharomyces pombe Involves Direct Interaction between Fcp1 and the Rpb4 Subunit of pol II. Mol. Cell. Biol. 22: 1577-1588 [Abstract] [Full Text]  
  • Cho, E.-J., Kobor, M. S., Kim, M., Greenblatt, J., Buratowski, S. (2001). Opposing effects of Ctk1 kinase and Fcp1 phosphatase at Ser 2 of the RNA polymerase II C-terminal domain. Genes Dev. 15: 3319-3329 [Abstract] [Full Text]  
  • Licciardo, P., Ruggiero, L., Lania, L., Majello, B. (2001). Transcription activation by targeted recruitment of the RNA polymerase II CTD phosphatase FCP1. Nucleic Acids Res 29: 3539-3545 [Abstract] [Full Text]  
  • Kamada, K., De Angelis, J., Roeder, R. G., Burley, S. K. (2001). Crystal structure of the C-terminal domain of the RAP74 subunit of human transcription factor IIF. Proc. Natl. Acad. Sci. USA 98: 3115-3120 [Abstract] [Full Text]  
  • Zhou, M., Nekhai, S., Bharucha, D. C., Kumar, A., Ge, H., Price, D. H., Egly, J.-M., Brady, J. N. (2001). TFIIH Inhibits CDK9 Phosphorylation during Human Immunodeficiency Virus Type 1 Transcription. J. Biol. Chem. 276: 44633-44640 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»