This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kokoska, R. J.
Right arrow Articles by Petes, T. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kokoska, R. J.
Right arrow Articles by Petes, T. D.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2000, p. 7490-7504, Vol. 20, No. 20
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Increased Rates of Genomic Deletions Generated by Mutations in the Yeast Gene Encoding DNA Polymerase delta  or by Decreases in the Cellular Levels of DNA Polymerase delta

Robert J. Kokoska, Lela Stefanovic, Jeremy DeMai, and Thomas D. Petes*

Department of Biology, Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280

Received 5 June 2000/Returned for modification 30 June 2000/Accepted 20 July 2000

In Saccharomyces cerevisiae, POL3 encodes the catalytic subunit of DNA polymerase delta . While yeast POL3 mutant strains that lack the proofreading exonuclease activity of the polymerase have a strong mutator phenotype, little is known regarding the role of other Pol3p domains in mutation avoidance. We identified a number of pol3 mutations in regions outside of the exonuclease domain that have a mutator phenotype, substantially elevating the frequency of deletions. These deletions appear to reflect an increased frequency of DNA polymerase slippage. In addition, we demonstrate that reduction in the level of wild-type DNA polymerase results in a similar mutator phenotype. Lowered levels of DNA polymerase also result in increased sensitivity to the DNA-damaging agent methyl methane sulfonate. We conclude that both the quantity and the quality of DNA polymerase delta  is important in ensuring genome stability.

* Corresponding author. Mailing address: Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599-3280. Phone: (919) 962-1445. Fax: (919) 962-8472. E-mail: tompetes{at}emailunc.edu.

Molecular and Cellular Biology, October 2000, p. 7490-7504, Vol. 20, No. 20
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Casper, A. M., Greenwell, P. W., Tang, W., Petes, T. D. (2009). Chromosome Aberrations Resulting From Double-Strand DNA Breaks at a Naturally Occurring Yeast Fragile Site Composed of Inverted Ty Elements Are Independent of Mre11p and Sae2p. Genetics 183: 423-439 [Abstract] [Full Text]  
  • Degtyareva, N. P., Chen, L., Mieczkowski, P., Petes, T. D., Doetsch, P. W. (2008). Chronic Oxidative DNA Damage Due to DNA Repair Defects Causes Chromosomal Instability in Saccharomyces cerevisiae. Mol. Cell. Biol. 28: 5432-5445 [Abstract] [Full Text]  
  • Lemoine, F. J., Degtyareva, N. P., Kokoska, R. J., Petes, T. D. (2008). Reduced Levels of DNA Polymerase {delta} Induce Chromosome Fragile Site Instability in Yeast. Mol. Cell. Biol. 28: 5359-5368 [Abstract] [Full Text]  
  • Mito, E., Mokhnatkin, J. V., Steele, M. C., Buettner, V. L., Sommer, S. S., Manthey, G. M., Bailis, A. M. (2008). Mutagenic and Recombinagenic Responses to Defective DNA Polymerase {delta} Are Facilitated by the Rev1 Protein in pol3-t Mutants of Saccharomyces cerevisiae. Genetics 179: 1795-1806 [Abstract] [Full Text]  
  • Silva, E. F. d., Reha-Krantz, L. J. (2007). DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase. Nucleic Acids Res 0: gkm591v1-12 [Abstract] [Full Text]  
  • Venkatesan, R. N., Hsu, J. J., Lawrence, N. A., Preston, B. D., Loeb, L. A. (2006). Mutator Phenotypes Caused by Substitution at a Conserved Motif A Residue in Eukaryotic DNA Polymerase {delta}. J. Biol. Chem. 281: 4486-4494 [Abstract] [Full Text]  
  • Sharma, S., Sommers, J. A., Brosh, R. M. Jr (2004). In vivo function of the conserved non-catalytic domain of Werner syndrome helicase in DNA replication. Hum Mol Genet 13: 2247-2261 [Abstract] [Full Text]  
  • Niimi, A., Limsirichaikul, S., Yoshida, S., Iwai, S., Masutani, C., Hanaoka, F., Kool, E. T., Nishiyama, Y., Suzuki, M. (2004). Palm Mutants in DNA Polymerases {alpha} and {eta} Alter DNA Replication Fidelity and Translesion Activity. Mol. Cell. Biol. 24: 2734-2746 [Abstract] [Full Text]  
  • Gutierrez, P. J. A., Wang, T. S.-F. (2003). Genomic Instability Induced by Mutations in Saccharomyces cerevisiae POL1. Genetics 165: 65-81 [Abstract] [Full Text]  
  • Ogawa, M., Limsirichaikul, S., Niimi, A., Iwai, S., Yoshida, S., Suzuki, M. (2003). Distinct Function of Conserved Amino Acids in the Fingers of Saccharomyces cerevisiae DNA Polymerase {alpha}. J. Biol. Chem. 278: 19071-19078 [Abstract] [Full Text]  
  • Galli, A., Cervelli, T., Schiestl, R. H. (2003). Characterization of the Hyperrecombination Phenotype of the pol3-t Mutation of Saccharomyces cerevisiae. Genetics 164: 65-79 [Abstract] [Full Text]  
  • Weetman, D., Hauser, L., Carvalho, G. R. (2002). Reconstruction of Microsatellite Mutation History Reveals a Strong and Consistent Deletion Bias in Invasive Clonal Snails, Potamopyrgus antipodarum. Genetics 162: 813-822 [Abstract] [Full Text]  
  • Craven, R. J., Greenwell, P. W., Dominska, M., Petes, T. D. (2002). Regulation of Genome Stability by TEL1 and MEC1, Yeast Homologs of the Mammalian ATM and ATR Genes. Genetics 161: 493-507 [Abstract] [Full Text]  
  • Cosson, B., Couturier, A., Chabelskaya, S., Kiktev, D., Inge-Vechtomov, S., Philippe, M., Zhouravleva, G. (2002). Poly(A)-Binding Protein Acts in Translation Termination via Eukaryotic Release Factor 3 Interaction and Does Not Influence [PSI+] Propagation. Mol. Cell. Biol. 22: 3301-3315 [Abstract] [Full Text]  
  • Huang, M.-E., Rio, A.-G., Galibert, M.-D., Galibert, F. (2002). Pol32, a Subunit of Saccharomyces cerevisiae DNA Polymerase {delta}, Suppresses Genomic Deletions and Is Involved in the Mutagenic Bypass Pathway. Genetics 160: 1409-1422 [Abstract] [Full Text]  
  • Sia, E. A., Dominska, M., Stefanovic, L., Petes, T. D. (2001). Isolation and Characterization of Point Mutations in Mismatch Repair Genes That Destabilize Microsatellites in Yeast. Mol. Cell. Biol. 21: 8157-8167 [Abstract] [Full Text]  
  • Corrette-Bennett, S. E., Mohlman, N. L., Rosado, Z., Miret, J. J., Hess, P. M., Parker, B. O., Lahue, R. S. (2001). Efficient repair of large DNA loops in Saccharomyces cerevisiae. Nucleic Acids Res 29: 4134-4143 [Abstract] [Full Text]  
  • Bebenek, K., Matsuda, T., Masutani, C., Hanaoka, F., Kunkel, T. A. (2001). Proofreading of DNA Polymerase eta -dependent Replication Errors. J. Biol. Chem. 276: 2317-2320 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»