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Molecular and Cellular Biology, November 2000, p. 7881-7892, Vol. 20, No. 21
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
In Yeast, the 3' Untranslated Region or the Presequence of
ATM1 Is Required for the Exclusive Localization of Its
mRNA to the Vicinity of Mitochondria
M.
Corral-Debrinski,*
C.
Blugeon, and
C.
Jacq
Laboratoire de Génétique Moléculaire, UMR CNRS
8541, Ecole Normale Supérieure, 75230 Paris, France
Received 24 April 2000/Returned for modification 24 May
2000/Accepted 7 August 2000
We isolated mitochondria from Saccharomyces cerevisiae
to selectively study polysomes bound to the mitochondrial surface. The
distribution of several mRNAs coding for mitochondrial proteins was
examined in free and mitochondrion-bound polysomes. Some mRNAs exclusively localize to mitochondrion-bound polysomes, such as the ones
coding for Atm1p, Cox10p, Tim44p, Atp2p, and Cot1p. In contrast, mRNAs
encoding Cox6p, Cox5a, Aac1p, and Mir1p are found enriched in free
cytoplasmic polysome fractions. Aac1p and Mir1p are transporters that
lack cleavable presequences. Sequences required for mRNA asymmetric
subcellular distribution were determined by analyzing the localization
of reporter mRNAs containing the presequence coding region and/or the
3'-untranslated region (3'UTR) of ATM1, a gene encoding an
ABC transporter of the mitochondrial inner membrane.
Biochemical analyses of mitochondrion-bound polysomes and direct
visualization of RNA localization in living yeast cells allowed us to
demonstrate that either the presequence coding region or the 3'UTR of
ATM1 is sufficient to allow the reporter mRNA to localize
to the vicinity of the mitochondrion, independently of its translation.
These data demonstrate that mRNA localization is one of the mechanisms
used, in yeast, for segregating mitochondrial proteins.
*
Corresponding author. Mailing address: Laboratoire de
Génétique Moleculaire, UMR CNRS 8541, Ecole Normale
Supérieure, 46 Rue d'Ulm, 75230 Paris, France. Phone:
33-1-44-32-39-39. Fax: 33-1-44-32-39-41. E-mail:
corral{at}biologie.ens.fr.
Molecular and Cellular Biology, November 2000, p. 7881-7892, Vol. 20, No. 21
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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