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Molecular and Cellular Biology, November 2000, p. 8157-8167, Vol. 20, No. 21
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Regulatory Networks Revealed by Transcriptional
Profiling of Damaged Saccharomyces cerevisiae Cells: Rpn4
Links Base Excision Repair with Proteasomes
Scott A.
Jelinsky,1
Preston
Estep,2
George M.
Church,2 and
Leona D.
Samson1,*
Cancer Cell Biology, Harvard School of Public
Health,1 and Department of Genetics,
Harvard Medical School,2 Boston, Massachusetts
02115
Received 31 May 2000/Returned for modification 13 July
2000/Accepted 4 August 2000
Exposure to carcinogenic alkylating agents, oxidizing agents, and
ionizing radiation modulates transcript levels for over one third of
Saccharomyces cerevisiae's 6,200 genes. Computational analysis delineates groups of coregulated genes whose upstream regions
bear known and novel regulatory sequence motifs. One group of
coregulated genes contain a number of DNA excision repair genes (including the MAG1 3-methyladenine DNA glycosylase gene)
and a large selection of protein degradation genes. Moreover,
transcription of these genes is modulated by the proteasome-associated
protein Rpn4, most likely via its binding to MAG1 upstream
repressor sequence 2-like elements, that turn out to be almost
identical to the recently identified proteasome-associated control
element (G. Mannhaupt, R. Schnall, V. Karpov, I. Vetter, and H. Feldmann, FEBS Lett. 450:27-34, 1999). We have identified a large
number of genes whose transcription is influenced by Rpn4p.
*
Corresponding author. Mailing address: Cancer Cell
Biology, Harvard School of Public Health, 665 Huntington Avenue,
Boston, MA 02115. Phone: (617) 432-1085. Fax: (617) 432-0400. E-mail: lsamson{at}hsph.harvard.edu.
Molecular and Cellular Biology, November 2000, p. 8157-8167, Vol. 20, No. 21
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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