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Molecular and Cellular Biology, November 2000, p. 8209-8219, Vol. 20, No. 21
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Profilin II Is Alternatively Spliced, Resulting in Profilin Isoforms That Are Differentially Expressed and Have Distinct Biochemical Properties

Anja Lambrechts,1 Attila Braun,2 Veronique Jonckheere,1 Attila Aszodi,2 Lorene M. Lanier,3 Johan Robbens,1 Inge Van Colen,1 Joël Vandekerckhove,1 Reinhard Fässler,2 and Christophe Ampe1,*

Department of Biochemistry, Ghent University and Flanders Interuniversity Institute for Biotechnology, 9000 Ghent, Belgium1; Department of Experimental Pathology, Lund University, 22 1 85 Lund, Sweden2; and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139-43073

Received 9 March 2000/Returned for modification 24 May 2000/Accepted 8 August 2000

We deduced the structure of the mouse profilin II gene. It contains five exons that can generate four different transcripts by alternative splicing. Two transcripts encode different profilin II isoforms (designated IIa and IIb) that have similar affinities for actin but different affinities for polyphosphoinositides and proline-rich sequences. Profilins IIa and IIb are also present in humans, suggesting that all mammals have three profilin isoforms. Profilin I is the major form in all tissues, except in the brain, where profilin IIa is most abundant. Profilin IIb appears to be a minor form, and its expression is restricted to a limited number of tissues, indicating that the alternative splicing is tightly regulated. Western blotting and whole-mount in situ hybridization show that, in contrast to the expression of profilin I, the expression level of profilin IIa is developmentally regulated. In situ hybridization of adult brain sections reveals overlapping expression patterns of profilins I and IIa.

* Corresponding author. Mailing address: Department of Biochemistry, Ghent University, B-9000 Ghent, Belgium. Phone: 32 9 2645306. Fax: 32 9 2645337. E-mail: champ{at}gengenp.rug.ac.be.

Molecular and Cellular Biology, November 2000, p. 8209-8219, Vol. 20, No. 21
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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