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Molecular and Cellular Biology, November 2000, p. 8319-8328, Vol. 20, No. 22
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Developmentally Regulated Rpd3p Homolog Specific to the Transcriptionally Active Macronucleus of Vegetative Tetrahymena thermophila

Emily A. Wiley,1,2,dagger Reiko Ohba,2 Meng-Chao Yao,1 and C. David Allis2,*

Fred Hutchinson Cancer Research Center, Seattle, Washington 98109,1 and Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia 22908-07332

Received 29 March 2000/Returned for modification 16 May 2000/Accepted 31 July 2000

A clear relationship exists between histone acetylation and transcriptional output, the balance of which is conferred by opposing histone acetyltransferases (HATs) and histone deacetylases (HDACs). To explore the role of HDAC activity in determining the transcriptional competency of chromatin, we have exploited the biological features of Tetrahymena as a model. Each vegetative cell contains two nuclei: a somatic, transcriptionally active macronucleus containing hyperacetylated chromatin and a transcriptionally silent, germ line micronucleus containing hypoacetylated histones. Using a PCR-based strategy, a deacetylase gene (named THD1) encoding a homolog of the yeast HDAC Rpd3p was cloned. Thd1p deacetylates all four core histones in vitro. It resides exclusively in the macronucleus during vegetative growth and is asymmetrically distributed to developing new macronuclei early in their differentiation during the sexual pathway. Together, these data are most consistent with a potential role for Thd1p in transcriptional regulation and suggest that histone deacetylation may be important for the differentiation of micronuclei into macronuclei during development.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Genetics, University of Virginia, Box 800733, Charlottesville, VA 22908-0733. Phone: (804) 243-6048. Fax: (804) 924-5069. E-mail: allis{at}virginia.edu.

dagger Present address: Department of Biology, Clapp Laboratory, Mount Holyoke College, South Hadley, MA 01075. Phone: (206) 667-4435.

Molecular and Cellular Biology, November 2000, p. 8319-8328, Vol. 20, No. 22
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Smith, J. J., Torigoe, S. E., Maxson, J., Fish, L. C., Wiley, E. A. (2008). A Class II Histone Deacetylase Acts on Newly Synthesized Histones in Tetrahymena. Eukaryot Cell 7: 471-482 [Abstract] [Full Text]  
  • Parker, K., Maxson, J., Mooney, A., Wiley, E. A. (2007). Class I Histone Deacetylase Thd1p Promotes Global Chromatin Condensation in Tetrahymena thermophila. Eukaryot Cell 6: 1913-1924 [Abstract] [Full Text]  
  • Stemm-Wolf, A. J., Morgan, G., Giddings, T. H. Jr., White, E. A., Marchione, R., McDonald, H. B., Winey, M. (2005). Basal Body Duplication and Maintenance Require One Member of the Tetrahymena thermophila Centrin Gene Family. Mol. Biol. Cell 16: 3606-3619 [Abstract] [Full Text]  
  • Wiley, E. A., Myers, T., Parker, K., Braun, T., Yao, M.-C. (2005). Class I Histone Deacetylase Thd1p Affects Nuclear Integrity in Tetrahymena thermophila. Eukaryot Cell 4: 981-990 [Abstract] [Full Text]  
  • Duharcourt, S., Yao, M.-C. (2002). Role of Histone Deacetylation in Developmentally Programmed DNA Rearrangements in Tetrahymena thermophila. Eukaryot Cell 1: 293-303 [Abstract] [Full Text]  


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