Uridylate Addition and RNA Ligation Contribute to the Specificity of Kinetoplastid Insertion RNA Editing

doi:10.1128/MCB.20.22.8447-8457.2000

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Igo, R. P.
	Articles by Stuart, K.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Igo, R. P., Jr.
	Articles by Stuart, K.

Previous Article | Next Article

Molecular and Cellular Biology, November 2000, p. 8447-8457, Vol. 20, No. 22
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Uridylate Addition and RNA Ligation Contribute to the Specificity of Kinetoplastid Insertion RNA Editing

Robert P. Igo Jr., Setareh S. Palazzo, Moffett L. K. Burgess, Aswini K. Panigrahi, and Kenneth Stuart^*

Seattle Biomedical Research Institute, Seattle, Washington 98109

Received 10 May 2000/Returned for modification 21 June 2000/Accepted 24 August 2000

RNA editing in Trypanosoma brucei inserts and deletes uridylates (U's) in mitochondrial pre-mRNAs under the direction of guideRNAs (gRNAs). We report here the development of a novel in vitroprecleaved editing assay and its use to study the gRNA specificityof the U addition and RNA ligation steps in insertion RNA editing.The 5' fragment of substrate RNA accumulated with the number ofadded U's specified by gRNA, and U addition products with morethan the specified number of U's were rare. U addition up to thenumber specified occurred in the absence of ligation, but accumulationof U addition products was slowed. The 5' fragments with the correctnumber of added U's were preferentially ligated, apparently byadenylylated RNA ligase since exogenously added ATP was not requiredand since ligation was eliminated by treatment with pyrophosphate.gRNA-specified U addition was apparent in the absence of ligationwhen the pre-mRNA immediately upstream of the editing site wassingle stranded and more so when it was base paired with gRNA.These results suggest that both the U addition and RNA ligationsteps contributed to the precision of RNAediting.

^* Corresponding author. Mailing address: Seattle Biomedical Research Institute, 4 Nickerson St., Seattle, WA 98109. Phone: (206)284-8846 x316. Fax: (206) 284-0313. E-mail: kstuart{at}u.washington.edu.

Molecular and Cellular Biology, November 2000, p. 8447-8457, Vol. 20, No. 22
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

Reifur, L., Koslowsky, D. J. (2008). Trypanosoma brucei ATPase subunit 6 mRNA bound to gA6-14 forms a conserved three-helical structure. RNA 14: 2195-2211 [Abstract] [Full Text]
Law, J. A., O'Hearn, S. F., Sollner-Webb, B. (2008). Trypanosoma brucei RNA editing protein TbMP42 (band VI) is crucial for the endonucleolytic cleavages but not the subsequent steps of U-deletion and U-insertion. RNA 14: 1187-1200 [Abstract] [Full Text]
Alatortsev, V. S., Cruz-Reyes, J., Zhelonkina, A. G., Sollner-Webb, B. (2008). Trypanosoma brucei RNA Editing: Coupled Cycles of U Deletion Reveal Processive Activity of the Editing Complex. Mol. Cell. Biol. 28: 2437-2445 [Abstract] [Full Text]
Tarun, S. Z. Jr, Schnaufer, A., Ernst, N. L., Proff, R., Deng, J., Hol, W., Stuart, K. (2008). KREPA6 is an RNA-binding protein essential for editosome integrity and survival of Trypanosoma brucei. RNA 14: 347-358 [Abstract] [Full Text]
Carnes, J., Trotter, J. R., Peltan, A., Fleck, M., Stuart, K. (2008). RNA Editing in Trypanosoma brucei Requires Three Different Editosomes. Mol. Cell. Biol. 28: 122-130 [Abstract] [Full Text]
Babbarwal, V. K., Fleck, M., Ernst, N. L., Schnaufer, A., Stuart, K. (2007). An essential role of KREPB4 in RNA editing and structural integrity of the editosome in Trypanosoma brucei. RNA 13: 737-744 [Abstract] [Full Text]
Cifuentes-Rojas, C., Pavia, P., Hernandez, A., Osterwisch, D., Puerta, C., Cruz-Reyes, J. (2007). Substrate Determinants for RNA Editing and Editing Complex Interactions at a Site for Full-round U Insertion. J. Biol. Chem. 282: 4265-4276 [Abstract] [Full Text]
Law, J. A., O'Hearn, S., Sollner-Webb, B. (2007). In Trypanosoma brucei RNA Editing, TbMP18 (Band VII) Is Critical for Editosome Integrity and for both Insertional and Deletional Cleavages. Mol. Cell. Biol. 27: 777-787 [Abstract] [Full Text]
Panigrahi, A. K., Ernst, N. L., Domingo, G. J., Fleck, M., Salavati, R., Stuart, K. D. (2006). Compositionally and functionally distinct editosomes in Trypanosoma brucei. RNA 12: 1038-1049 [Abstract] [Full Text]
Yu, L. E., Koslowsky, D. J. (2006). Interactions of mRNAs and gRNAs involved in trypanosome mitochondrial RNA editing: Structure probing of a gRNA bound to its cognate mRNA. RNA 12: 1050-1060 [Abstract] [Full Text]
Salavati, R., Ernst, N. L., O'Rear, J., Gilliam, T., Tarun, S. Jr., Stuart, K. (2006). KREPA4, an RNA binding protein essential for editosome integrity and survival of Trypanosoma brucei. RNA 12: 819-831 [Abstract] [Full Text]
ZHELONKINA, A. G., O'HEARN, S. F., LAW, J. A., CRUZ-REYES, J., HUANG, C. E., ALATORTSEV, V. S., SOLLNER-WEBB, B. (2006). T. brucei RNA editing: Action of the U-insertional TUTase within a U-deletion cycle.. RNA 12: 476-487 [Abstract] [Full Text]
Cifuentes-Rojas, C., Halbig, K., Sacharidou, A., De Nova-Ocampo, M., Cruz-Reyes, J. (2005). Minimal pre-mRNA substrates with natural and converted sites for full-round U insertion and U deletion RNA editing in trypanosomes. Nucleic Acids Res 33: 6610-6620 [Abstract] [Full Text]
Carnes, J., Trotter, J. R., Ernst, N. L., Steinberg, A., Stuart, K. (2005). An essential RNase III insertion editing endonuclease in Trypanosoma brucei. Proc. Natl. Acad. Sci. USA 102: 16614-16619 [Abstract] [Full Text]
Law, J. A., Huang, C. E., O'Hearn, S. F., Sollner-Webb, B. (2005). In Trypanosoma brucei RNA Editing, Band II Enables Recognition Specifically at Each Step of the U Insertion Cycle. Mol. Cell. Biol. 25: 2785-2794 [Abstract] [Full Text]
Vondruskova, E., van den Burg, J., Zikova, A., Ernst, N. L., Stuart, K., Benne, R., Lukes, J. (2005). RNA Interference Analyses Suggest a Transcript-specific Regulatory Role for Mitochondrial RNA-binding Proteins MRP1 and MRP2 in RNA Editing and Other RNA Processing in Trypanosoma brucei. J. Biol. Chem. 280: 2429-2438 [Abstract] [Full Text]
Kang, X., Rogers, K., Gao, G., Falick, A. M., Zhou, S., Simpson, L. (2005). Reconstitution of uridine-deletion precleaved RNA editing with two recombinant enzymes. Proc. Natl. Acad. Sci. USA 102: 1017-1022 [Abstract] [Full Text]
GOLDEN, D. E., HAJDUK, S. L. (2005). The 3'-untranslated region of cytochrome oxidase II mRNA functions in RNA editing of African trypanosomes exclusively as a cis guide RNA. RNA 11: 29-37 [Abstract] [Full Text]
Aphasizhev, R., Aphasizheva, I., Simpson, L. (2003). From the Cover: A tale of two TUTases. Proc. Natl. Acad. Sci. USA 100: 10617-10622 [Abstract] [Full Text]
Domingo, G. J., Palazzo, S. S., Wang, B., Pannicucci, B., Salavati, R., Stuart, K. D. (2003). Dyskinetoplastic Trypanosoma brucei Contains Functional Editing Complexes. Eukaryot Cell 2: 569-577 [Abstract] [Full Text]
Wang, B., Ernst, N. L., Palazzo, S. S., Panigrahi, A. K., Salavati, R., Stuart, K. (2003). TbMP44 Is Essential for RNA Editing and Structural Integrity of the Editosome in Trypanosoma brucei. Eukaryot Cell 2: 578-587 [Abstract] [Full Text]
PAI, R. D., OPPEGARD, L. M., CONNELL, G. J. (2003). Sequence and structural requirements for optimal guide RNA-directed insertional editing within Leishmania tarentolae. RNA 9: 469-483 [Abstract] [Full Text]
SIMPSON, L., SBICEGO, S., APHASIZHEV, R. (2003). Uridine insertion/deletion RNA editing in trypanosome mitochondria: A complex business. RNA 9: 265-276 [Abstract] [Full Text]
APHASIZHEV, R., APHASIZHEVA, I., NELSON, R. E., SIMPSON, L. (2003). A 100-kD complex of two RNA-binding proteins from mitochondria of Leishmania tarentolae catalyzes RNA annealing and interacts with several RNA editing components. RNA 9: 62-76 [Abstract] [Full Text]
Cruz-Reyes, J., Zhelonkina, A. G., Huang, C. E., Sollner-Webb, B. (2002). Distinct Functions of Two RNA Ligases in Active Trypanosoma brucei RNA Editing Complexes. Mol. Cell. Biol. 22: 4652-4660 [Abstract] [Full Text]
Huang, C. E., O'Hearn, S. F., Sollner-Webb, B. (2002). Assembly and Function of the RNA Editing Complex in Trypanosoma brucei Requires Band III Protein. Mol. Cell. Biol. 22: 3194-3203 [Abstract] [Full Text]
Igo, R. P. Jr., Lawson, S. D., Stuart, K. (2002). RNA Sequence and Base Pairing Effects on Insertion Editing in Trypanosoma brucei. Mol. Cell. Biol. 22: 1567-1576 [Abstract] [Full Text]
Igo, R. P. Jr., Weston, D. S., Ernst, N. L., Panigrahi, A. K., Salavati, R., Stuart, K. (2002). Role of Uridylate-Specific Exoribonuclease Activity in Trypanosoma brucei RNA Editing. Eukaryot Cell 1: 112-118 [Abstract] [Full Text]
Panigrahi, A. K., Schnaufer, A., Carmean, N., Igo, R. P. Jr., Gygi, S. P., Ernst, N. L., Palazzo, S. S., Weston, D. S., Aebersold, R., Salavati, R., Stuart, K. D. (2001). Four Related Proteins of the Trypanosoma brucei RNA Editing Complex. Mol. Cell. Biol. 21: 6833-6840 [Abstract] [Full Text]
Cruz-Reyes, J., Zhelonkina, A., Rusche, L., Sollner-Webb, B. (2001). Trypanosome RNA Editing: Simple Guide RNA Features Enhance U Deletion 100-Fold. Mol. Cell. Biol. 21: 884-892 [Abstract] [Full Text]
Panigrahi, A. K., Gygi, S. P., Ernst, N. L., Igo, R. P. Jr., Palazzo, S. S., Schnaufer, A., Weston, D. S., Carmean, N., Salavati, R., Aebersold, R., Stuart, K. D. (2001). Association of Two Novel Proteins, TbMP52 and TbMP48, with the Trypanosoma brucei RNA Editing Complex. Mol. Cell. Biol. 21: 380-389 [Abstract] [Full Text]

J. Bacteriol.	J. Virol.	Eukaryot. Cell

Microbiol. Mol. Biol. Rev.	Clin. Vaccine Immunol.	All ASM Journals