This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lin, K.-I
Right arrow Articles by Calame, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lin, K.-I
Right arrow Articles by Calame, K.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, December 2000, p. 8684-8695, Vol. 20, No. 23
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Repression of c-myc Is Necessary but Not Sufficient for Terminal Differentiation of B Lymphocytes In Vitro

Kuo-I Lin,1 Yi Lin,1 and Kathryn Calame1,2,*

Department of Microbiology1 and Department of Biochemistry & Molecular Biophysics,2 College of Physicians and Surgeons, Columbia University, New York, New York 10032

Received 25 May 2000/Returned for modification 30 June 2000/Accepted 4 September 2000

The importance of c-myc as a target of the Blimp-1 repressor has been studied in BCL-1 cells, in which Blimp-1 is sufficient to trigger terminal B-cell differentiation. Our data show that Blimp-1-dependent repression of c-myc is required for BCL-1 differentiation, since constitutive expression of c-Myc blocked differentiation. Furthermore, ectopic expression of cyclin E mimicked the effects of c-Myc on both proliferation and differentiation, indicating that the ability of c-Myc to drive proliferation is responsible for blocking BCL-1 differentiation. However, inhibition of c-Myc by a dominant negative form was not sufficient to drive BCL-1 differentiation. Thus, during Blimp-1-dependent plasma cell differentiation, repression of c-myc is necessary but not sufficient, demonstrating the existence of additional Blimp-1 target genes.


* Corresponding author. Mailing address: Department of Microbiology, HHSC 1202, Columbia University College of Physicians and Surgeons, 701 W. 168th St., New York, NY 10032. Phone: (212) 305-3504. Fax: (212) 305-1468. E-mail: KLC1{at}columbia.edu.


Molecular and Cellular Biology, December 2000, p. 8684-8695, Vol. 20, No. 23
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Tsai, C.-M., Chiu, Y.-K., Hsu, T.-L., Lin, I-Y., Hsieh, S.-L., Lin, K.-I (2008). Galectin-1 Promotes Immunoglobulin Production during Plasma Cell Differentiation. J. Immunol. 181: 4570-4579 [Abstract] [Full Text]  
  • Lin, F.-R., Kuo, H.-K., Ying, H.-Y., Yang, F.-H., Lin, K.-I (2007). Induction of Apoptosis in Plasma Cells by B Lymphocyte Induced Maturation Protein-1 Knockdown. Cancer Res. 67: 11914-11923 [Abstract] [Full Text]  
  • Gong, D., Malek, T. R. (2007). Cytokine-Dependent Blimp-1 Expression in Activated T Cells Inhibits IL-2 Production. J. Immunol. 178: 242-252 [Abstract] [Full Text]  
  • Konforte, D., Paige, C. J. (2006). Identification of Cellular Intermediates and Molecular Pathways Induced by IL-21 in Human B Cells. J. Immunol. 177: 8381-8392 [Abstract] [Full Text]  
  • Tam, W., Gomez, M., Chadburn, A., Lee, J. W., Chan, W. C., Knowles, D. M. (2006). Mutational analysis of PRDM1 indicates a tumor-suppressor role in diffuse large B-cell lymphomas. Blood 107: 4090-4100 [Abstract] [Full Text]  
  • Zwollo, P., Cole, S., Bromage, E., Kaattari, S. (2005). B Cell Heterogeneity in the Teleost Kidney: Evidence for a Maturation Gradient from Anterior to Posterior Kidney. J. Immunol. 174: 6608-6616 [Abstract] [Full Text]  
  • Guedez, L., Martinez, A., Zhao, S., Vivero, A., Pittaluga, S., Stetler-Stevenson, M., Raffeld, M., Stetler-Stevenson, W. G. (2005). Tissue inhibitor of metalloproteinase 1 (TIMP-1) promotes plasmablastic differentiation of a Burkitt lymphoma cell line: implications in the pathogenesis of plasmacytic/plasmablastic tumors. Blood 105: 1660-1668 [Abstract] [Full Text]  
  • Kallies, A., Hasbold, J., Tarlinton, D. M., Dietrich, W., Corcoran, L. M., Hodgkin, P. D., Nutt, S. L. (2004). Plasma Cell Ontogeny Defined by Quantitative Changes in Blimp-1 Expression. JEM 200: 967-977 [Abstract] [Full Text]  
  • Tunyaplin, C., Shaffer, A. L., Angelin-Duclos, C. D., Yu, X., Staudt, L. M., Calame, K. L. (2004). Direct Repression of prdm1 by Bcl-6 Inhibits Plasmacytic Differentiation. J. Immunol. 173: 1158-1165 [Abstract] [Full Text]  
  • Raman, V. S., Akondy, R. S., Rath, S., Bal, V., George, A. (2003). Ligation of CD27 on B Cells In Vivo during Primary Immunization Enhances Commitment to Memory B Cell Responses. J. Immunol. 171: 5876-5881 [Abstract] [Full Text]  
  • Tarte, K., Zhan, F., De Vos, J., Klein, B., Shaughnessy, J. Jr (2003). Gene expression profiling of plasma cells and plasmablasts: toward a better understanding of the late stages of B-cell differentiation. Blood 102: 592-600 [Abstract] [Full Text]  
  • Underhill, G. H., George, D., Bremer, E. G., Kansas, G. S. (2003). Gene expression profiling reveals a highly specialized genetic program of plasma cells. Blood 101: 4013-4021 [Abstract] [Full Text]  
  • Gyory, I., Fejer, G., Ghosh, N., Seto, E., Wright, K. L. (2003). Identification of a Functionally Impaired Positive Regulatory Domain I Binding Factor 1 Transcription Repressor in Myeloma Cell Lines. J. Immunol. 170: 3125-3133 [Abstract] [Full Text]  
  • Lin, K.-I, Angelin-Duclos, C., Kuo, T. C., Calame, K. (2002). Blimp-1-Dependent Repression of Pax-5 Is Required for Differentiation of B Cells to Immunoglobulin M-Secreting Plasma Cells. Mol. Cell. Biol. 22: 4771-4780 [Abstract] [Full Text]  
  • O'Connor, B. P., Cascalho, M., Noelle, R. J. (2002). Short-lived and Long-lived Bone Marrow Plasma Cells Are Derived from a Novel Precursor Population. JEM 195: 737-745 [Abstract] [Full Text]  
  • Medico, E., Gentile, A., Celso, C. L., Williams, T. A., Gambarotta, G., Trusolino, L., Comoglio, P. M. (2001). Osteopontin Is an Autocrine Mediator of Hepatocyte Growth Factor-induced Invasive Growth. Cancer Res. 61: 5861-5868 [Abstract] [Full Text]