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Molecular and Cellular Biology, December 2000, p. 8684-8695, Vol. 20, No. 23
Department of
Microbiology1 and Department of
Biochemistry & Molecular Biophysics,2 College of
Physicians and Surgeons, Columbia University, New York, New York 10032
Received 25 May 2000/Returned for modification 30 June
2000/Accepted 4 September 2000
The importance of c-myc as a target of the Blimp-1
repressor has been studied in BCL-1 cells, in which Blimp-1 is
sufficient to trigger terminal B-cell differentiation. Our data show
that Blimp-1-dependent repression of c-myc is required for
BCL-1 differentiation, since constitutive expression of c-Myc blocked
differentiation. Furthermore, ectopic expression of cyclin E mimicked
the effects of c-Myc on both proliferation and differentiation,
indicating that the ability of c-Myc to drive proliferation is
responsible for blocking BCL-1 differentiation. However, inhibition of
c-Myc by a dominant negative form was not sufficient to drive BCL-1 differentiation. Thus, during Blimp-1-dependent plasma cell
differentiation, repression of c-myc is necessary but not
sufficient, demonstrating the existence of additional Blimp-1 target genes.
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Repression of c-myc Is Necessary but Not
Sufficient for Terminal Differentiation of B Lymphocytes In
Vitro
*
Corresponding author. Mailing address: Department of
Microbiology, HHSC 1202, Columbia University College of Physicians and Surgeons, 701 W. 168th St., New York, NY 10032. Phone: (212) 305-3504. Fax: (212) 305-1468. E-mail: KLC1{at}columbia.edu.
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