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Molecular and Cellular Biology, December 2000, p. 8958-8968, Vol. 20, No. 23
School of Biological Sciences, Seoul National
University, Seoul 151-742,1 and
Department of Genetic Engineering, Chosun University, Kwangju
501-759,2 Republic of Korea
Received 28 June 2000/Returned for modification 24 July
2000/Accepted 12 September 2000
The Schizosaccharomyces pombe DNA repair gene
rhp51+ encodes a RecA-like protein with the
DNA-dependent ATPase activity required for homologous recombination.
The level of the rhp51+ transcript is increased
by a variety of DNA-damaging agents. Its promoter has two
cis-acting DNA damage-responsive elements (DREs)
responsible for DNA damage inducibility. Here we report identification
of Rdp1, which regulates rhp51+ expression
through the DRE of rhp51+. The protein contains
a zinc finger and a polyalanine tract similar to ones previously
implicated in DNA binding and transactivation or repression,
respectively. In vitro footprinting and competitive binding assays
indicate that the core consensus sequences (NGG/TTG/A) of DRE are
crucial for the binding of Rdp1. Mutations of both DRE1 and DRE2
affected the damage-induced expression of
rhp51+, indicating that both DREs are required
for transcriptional activation. In addition, mutations in the DREs
significantly reduced survival rates after exposure to DNA-damaging
agents, demonstrating that the damage response of
rhp51+ enhances the cellular repair capacity.
Surprisingly, haploid cells containing a complete rdp1
deletion could not be recovered, indicating that
rdp1+ is essential for cell viability and
implying the existence of other target genes. Furthermore, the DNA
damage-dependent expression of rhp51+ was
significantly reduced in checkpoint mutants, raising the possibility
that Rdp1 may mediate damage checkpoint-dependent transcription of
rhp51+.
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Rdp1, a Novel Zinc Finger Protein, Regulates the
DNA Damage Response of rhp51+ from
Schizosaccharomyces pombe
*
Corresponding author. Mailing address: School of
Biological Sciences, Seoul National University, Seoul 151-742, Republic
of Korea. Phone: (82-2) 880-6689. Fax: (82-2) 887-6279. E-mail:
sdpark{at}plaza.snu.ac.kr.
Molecular and Cellular Biology, December 2000, p. 8958-8968, Vol. 20, No. 23
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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