This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kang, L. E.
Right arrow Articles by Symington, L. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kang, L. E.
Right arrow Articles by Symington, L. S.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, December 2000, p. 9162-9172, Vol. 20, No. 24
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Aberrant Double-Strand Break Repair in rad51 Mutants of Saccharomyces cerevisiae

Leslie E. Kang and Lorraine S. Symington*

Department of Microbiology and Institute of Cancer Research, Columbia University College of Physicians and Surgeons, New York, New York 10032

Received 10 July 2000/Returned for modification 17 August 2000/Accepted 21 September 2000

A number of studies of Saccharomyces cerevisiae have revealed RAD51-independent recombination events. These include spontaneous and double-strand break-induced recombination between repeated sequences, and capture of a chromosome arm by break-induced replication. Although recombination between inverted repeats is considered to be a conservative intramolecular event, the lack of requirement for RAD51 suggests that repair can also occur by a nonconservative mechanism. We propose a model for RAD51-independent recombination by one-ended strand invasion coupled to DNA synthesis, followed by single-strand annealing. The Rad1/Rad10 endonuclease is required to trim intermediates formed during single-strand annealing and thus was expected to be required for RAD51-independent events by this model. Double-strand break repair between plasmid-borne inverted repeats was less efficient in rad1 rad51 double mutants than in rad1 and rad51 strains. In addition, repair events were delayed and frequently associated with plasmid loss. Furthermore, the repair products recovered from the rad1 rad51 strain were primarily in the crossover configuration, inconsistent with conservative models for mitotic double-strand break repair.

* Corresponding author. Mailing address: Department of Microbiology and Institute of Cancer Research, Columbia University College of Physicians and Surgeons, 701 W. 168th St., New York, NY 10032. Phone: (212) 305-4793. Fax: (212) 305-1741. E-mail: lss5{at}columbia.edu.

Molecular and Cellular Biology, December 2000, p. 9162-9172, Vol. 20, No. 24
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jain, S., Sugawara, N., Lydeard, J., Vaze, M., Tanguy Le Gac, N., Haber, J. E. (2009). A recombination execution checkpoint regulates the choice of homologous recombination pathway during DNA double-strand break repair. Genes Dev. 23: 291-303 [Abstract] [Full Text]  
  • Lyndaker, A. M., Goldfarb, T., Alani, E. (2008). Mutants Defective in Rad1-Rad10-Slx4 Exhibit a Unique Pattern of Viability During Mating-Type Switching in Saccharomyces cerevisiae. Genetics 179: 1807-1821 [Abstract] [Full Text]  
  • Wu, Y., Kantake, N., Sugiyama, T., Kowalczykowski, S. C. (2008). Rad51 Protein Controls Rad52-mediated DNA Annealing. J. Biol. Chem. 283: 14883-14892 [Abstract] [Full Text]  
  • Pohl, T. J., Nickoloff, J. A. (2008). Rad51-Independent Interchromosomal Double-Strand Break Repair by Gene Conversion Requires Rad52 but Not Rad55, Rad57, or Dmc1. Mol. Cell. Biol. 28: 897-906 [Abstract] [Full Text]  
  • Cortes-Ledesma, F., Tous, C., Aguilera, A. (2007). Different genetic requirements for repair of replication-born double-strand breaks by sister-chromatid recombination and break-induced replication. Nucleic Acids Res 35: 6560-6570 [Abstract] [Full Text]  
  • Tsukamoto, M., Yamashita, K., Miyazaki, T., Shinohara, M., Shinohara, A. (2003). The N-Terminal DNA-Binding Domain of Rad52 Promotes RAD51-Independent Recombination in Saccharomyces cerevisiae. Genetics 165: 1703-1715 [Abstract] [Full Text]  
  • Spell, R. M., Jinks-Robertson, S. (2003). Role of Mismatch Repair in the Fidelity of RAD51- and RAD59-Dependent Recombination in Saccharomyces cerevisiae. Genetics 165: 1733-1744 [Abstract] [Full Text]  
  • Schmuckli-Maurer, J., Rolfsmeier, M., Nguyen, H., Heyer, W.-D. (2003). Genome instability in rad54 mutants of Saccharomyces cerevisiae. Nucleic Acids Res 31: 1013-1023 [Abstract] [Full Text]  
  • Symington, L. S. (2002). Role of RAD52 Epistasis Group Genes in Homologous Recombination and Double-Strand Break Repair. Microbiol. Mol. Biol. Rev. 66: 630-670 [Abstract] [Full Text]  
  • Rattray, A. J., Shafer, B. K., McGill, C. B., Strathern, J. N. (2002). The Roles of REV3 and RAD57 in Double-Strand-Break-Repair-Induced Mutagenesis of Saccharomyces cerevisiae. Genetics 162: 1063-1077 [Abstract] [Full Text]  
  • Gonzalez-Barrera, S., Garcia-Rubio, M., Aguilera, A. (2002). Transcription and Double-Strand Breaks Induce Similar Mitotic Recombination Events in Saccharomyces cerevisiae. Genetics 162: 603-614 [Abstract] [Full Text]  
  • Ira, G., Haber, J. E. (2002). Characterization of RAD51-Independent Break-Induced Replication That Acts Preferentially with Short Homologous Sequences. Mol. Cell. Biol. 22: 6384-6392 [Abstract] [Full Text]  
  • Freedman, J. A., Jinks-Robertson, S. (2002). Genetic Requirements for Spontaneous and Transcription-Stimulated Mitotic Recombination in Saccharomyces cerevisiae. Genetics 162: 15-27 [Abstract] [Full Text]  
  • Stark, J. M., Hu, P., Pierce, A. J., Moynahan, M. E., Ellis, N., Jasin, M. (2002). ATP Hydrolysis by Mammalian RAD51 Has a Key Role during Homology-directed DNA Repair. J. Biol. Chem. 277: 20185-20194 [Abstract] [Full Text]  
  • Davis, A. P., Symington, L. S. (2001). The Yeast Recombinational Repair Protein Rad59 Interacts With Rad52 and Stimulates Single-Strand Annealing. Genetics 159: 515-525 [Abstract] [Full Text]  
  • Malagon, F., Aguilera, A. (2001). Yeast spt6-140 Mutation, Affecting Chromatin and Transcription, Preferentially Increases Recombination in Which Rad51p-Mediated Strand Exchange Is Dispensable. Genetics 158: 597-611 [Abstract] [Full Text]  
  • Malkova, A., Signon, L., Schaefer, C. B., Naylor, M. L., Theis, J. F., Newlon, C. S., Haber, J. E. (2001). RAD51-independent break-induced replication to repair a broken chromosome depends on a distant enhancer site. Genes Dev. 15: 1055-1060 [Abstract] [Full Text]  
  • Signon, L., Malkova, A., Naylor, M. L., Klein, H., Haber, J. E. (2001). Genetic Requirements for RAD51- and RAD54-Independent Break-Induced Replication Repair of a Chromosomal Double-Strand Break. Mol. Cell. Biol. 21: 2048-2056 [Abstract] [Full Text]  
  • Kagawa, W., Kurumizaka, H., Ikawa, S., Yokoyama, S., Shibata, T. (2001). Homologous Pairing Promoted by the Human Rad52 Protein. J. Biol. Chem. 276: 35201-35208 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»