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Molecular and Cellular Biology, December 2000, p. 9173-9181, Vol. 20, No. 24
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

DNA Damage in the Nucleosome Core Is Refractory to Repair by Human Excision Nuclease

Ryujiro Hara, Jinyao Mo, and Aziz Sancar*

Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599

Received 17 August 2000/Returned for modification 27 September 2000/Accepted 29 September 2000

To investigate the effect of nucleosomes on nucleotide excision repair in humans, we prepared a mononucleosome containing a (6-4) photoproduct in the nucleosome core and examined its repair with the reconstituted human excision nuclease system and with cell extracts. Nucleosomal DNA is repaired at a rate of about 10% of that for naked DNA in both systems. These results are in agreement with in vivo data showing a considerably slower rate of repair of overall genomic DNA relative to that for transcriptionally active DNA. Furthermore, our results indicate that the first-order packing of DNA in nucleosomes is a primary determinant of slow repair of DNA in chromatin.

* Corresponding author. Mailing address: Department of Biochemistry and Biophysics, Mary Ellen Jones Building, CB#7260, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7260. Phone: (919) 962-0115. Fax: (919) 843-8627. E-mail: Aziz_Sancar{at}med.unc.edu.

Molecular and Cellular Biology, December 2000, p. 9173-9181, Vol. 20, No. 24
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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