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Molecular and Cellular Biology, December 2000, p. 9423-9433, Vol. 20, No. 24
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Isolation and Characterization of
Suv39h2, a Second Histone H3 Methyltransferase Gene That
Displays Testis-Specific Expression
Dónal
O'Carroll,1
Harry
Scherthan,2
Antoine H. F. M.
Peters,1
Susanne
Opravil,1
Andrew R.
Haynes,3
Götz
Laible,1,
Stephen
Rea,1
Manfred
Schmid,1
Angelika
Lebersorger,1
Martin
Jerratsch,2
Lydia
Sattler,4
Marie G.
Mattei,5
Paul
Denny,3
Stephen D. M.
Brown,3
Dieter
Schweizer,4 and
Thomas
Jenuwein1,*
Research Institute of Molecular Pathology at
The Vienna Biocenter,1 and Institute of
Botany, University of Vienna,4 A-1030
Vienna, Austria; Institute of Human Biology, University of
Kaiserslautern, D-67663 Kaiserslautern,
Germany2; Mouse Genome Centre and
Mammalian Genetics Unit, Medical Research Council, Harwell, Oxon OX11
ORD, United Kingdom3; and INSERM
U406, Génétique Médicale et Développement,
13385 Marseille Cedex 5, France5
Received 26 September 2000/Accepted 28 September 2000
Higher-order chromatin has been implicated in epigenetic gene
control and in the functional organization of chromosomes. We have
recently discovered mouse (Suv39h1) and human (SUV39H1) histone H3
lysine 9-selective methyltransferases (Suv39h HMTases) and shown that
they modulate chromatin dynamics in somatic cells. We describe here the
isolation, chromosomal assignment, and characterization of a second
murine gene, Suv39h2. Like Suv39h1,
Suv39h2 encodes an H3 HMTase that shares 59% identity with
Suv39h1 but which differs by the presence of a highly basic N terminus.
Using fluorescent in situ hybridization and haplotype analysis, the
Suv39h2 locus was mapped to the subcentromeric region of
mouse chromosome 2, whereas the Suv39h1 locus resides at
the tip of the mouse X chromosome. Notably, although both
Suv39h loci display overlapping expression profiles during
mouse embryogenesis, Suv39h2 transcripts remain specifically expressed in adult testes. Immunolocalization of Suv39h2
protein during spermatogenesis indicates enriched distribution at the
heterochromatin from the leptotene to the round spermatid stage.
Moreover, Suv39h2 specifically accumulates with chromatin of the sex
chromosomes (XY body) which undergo transcriptional silencing during
the first meiotic prophase. These data are consistent with redundant
enzymatic roles for Suv39h1 and Suv39h2 during mouse development and suggest an additional function of the Suv39h2 HMTase in organizing meiotic heterochromatin that may even impart an
epigenetic imprint to the male germ line.
*
Corresponding author. Mailing address: Research
Institute of Molecular Pathology at the Vienna Biocenter, Dr. Bohrgasse
7, A-1030 Vienna, Austria. Phone: (43/1) 797-30-474. Fax: (43/1) 798-7153. E-mail: jenuwein{at}nt.imp.univie.ac.at.

Present address: Dairy Science Group, AgResearch, Hamilton, New
Zealand.
Molecular and Cellular Biology, December 2000, p. 9423-9433, Vol. 20, No. 24
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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