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Molecular and Cellular Biology, February 2000, p. 947-956, Vol. 20, No. 3
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Complex Effects of Naturally Occurring Mutations in the JAK3 Pseudokinase Domain: Evidence for Interactions between the Kinase and Pseudokinase Domains

Min Chen,1,* Alan Cheng,2 Fabio Candotti,3 Yong-Jie Zhou,1 Anka Hymel,1 Anders Fasth,4 Luigi D. Notarangelo,5 and John J. O'Shea1

Lymphocyte Cell Biology Section, Arthritis and Rheumatism Branch, National Institute of Arthritis, Musculoskeletal and Skin Diseases,1 Howard Hughes Medical Institute---National Institutes of Health Research Scholars Program,2 Clinical Gene Therapy Branch, National Human Genome Research Institute,3 National Institutes of Health, Bethesda, Maryland 20892; Department of Pediatrics, University of Goteborg, SE-41685 Goteborg, Sweden4; and Department of Pediatrics, University of Brescia, I-25123 Brescia, Italy5

Received 5 October 1999/Accepted 5 November 1999

The structure of Janus kinases (JAKs) is unique among protein tyrosine kinases in having tandem, nonidentical kinase and pseudokinase domains. Despite its conservation in evolution, however, the function of the pseudokinase domain remains poorly understood. Lack of JAK3 expression results in severe combined immunodeficiency (SCID). In this study, we analyze two SCID patients with mutations in the JAK3 pseudokinase domain, which allows for protein expression but disrupts the regulation of the kinase activity. Specifically, these mutant forms of JAK3 had undetectable kinase activity in vitro but were hyperphosphorylated both in patients' Epstein-Barr virus-transformed B cells and when overexpressed in COS7 cells. Moreover, reconstitution of cells with these mutants demonstrated that, although they were constitutively phosphorylated basally, they were unable to transmit cytokine-dependent signals. Further analysis showed that the isolated catalytic domain of JAK3 was functional whereas either the addition of the pseudokinase domain or its deletion from the full-length molecule reduced catalytic activity. Through coimmunoprecipitation of the isolated pseudokinase domain with the isolated catalytic domain, we provide the first evidence that these two domains interact. Furthermore, whereas the wild-type pseudokinase domain modestly inhibited kinase domain-mediated STAT5 phosphorylation, the patient-derived mutants markedly inhibited this phosphorylation. We thus conclude that the JAK3 pseudokinase domain is essential for JAK3 function by regulating its catalytic activity and autophosphorylation. We propose a model in which this occurs via intramolecular interaction with the kinase domain and that increased inhibition of kinase activity by the pseudokinase domain likely contributes to the disease pathogenesis in these two patients.

* Corresponding author. Mailing address: LCBS/ARB/NIAMS/NIH, 10/9N262, 10 Center Dr., MSC-1820, Bethesda, MD 20892. Phone: (301) 496-2541. Fax: (301) 402-0012. E-mail: chenm{at}arb.niams.nih.gov.

Molecular and Cellular Biology, February 2000, p. 947-956, Vol. 20, No. 3
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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