This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Trebitsh, T.
Right arrow Articles by Danon, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Trebitsh, T.
Right arrow Articles by Danon, A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, February 2000, p. 1116-1123, Vol. 20, No. 4
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Translation of Chloroplast psbA mRNA Is Modulated in the Light by Counteracting Oxidizing and Reducing Activities

Tova Trebitsh, Alex Levitan, Anat Sofer, and Avihai Danon*

Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel

Received 7 September 1999/Returned for modification 20 October 1999/Accepted 10 November 1999

Light has been proposed to stimulate the translation of Chlamydomonas reinhardtii chloroplast psbA mRNA by activating a protein complex associated with the 5' untranslated region of this mRNA. The protein complex contains a redox-active regulatory site responsive to thioredoxin. We identified RB60, a protein disulfide isomerase-like member of the protein complex, as carrying the redox-active regulatory site composed of vicinal dithiol. We assayed in parallel the redox state of RB60 and translation of psbA mRNA in intact chloroplasts. Light activated the specific oxidation of RB60, on the one hand, and reduced RB60, probably via the ferredoxin-thioredoxin system, on the other. Higher light intensities increased the pool of reduced RB60 and the rate of psbA mRNA translation, suggesting that a counterbalanced action of reducing and oxidizing activities modulates the translation of psbA mRNA in parallel with fluctuating light intensities. In the dark, chemical reduction of the vicinal dithiol site did not activate translation. These results suggest a mechanism by which light primes redox-regulated translation by an unknown mechanism and then the rate of translation is determined by the reduction-oxidation of a sensor protein located in a complex bound to the 5' untranslated region of the chloroplast mRNA.

* Corresponding author. Mailing address: Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel. Phone: 972-8-934-2382. Fax: 972-8-934-4181. E-mail: Avihai.Danon{at}weizmann.ac.il.

Molecular and Cellular Biology, February 2000, p. 1116-1123, Vol. 20, No. 4
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Brautigam, K., Dietzel, L., Kleine, T., Stroher, E., Wormuth, D., Dietz, K.-J., Radke, D., Wirtz, M., Hell, R., Dormann, P., Nunes-Nesi, A., Schauer, N., Fernie, A. R., Oliver, S. N., Geigenberger, P., Leister, D., Pfannschmidt, T. (2009). Dynamic Plastid Redox Signals Integrate Gene Expression and Metabolism to Induce Distinct Metabolic States in Photosynthetic Acclimation in Arabidopsis. Plant Cell 21: 2715-2732 [Abstract] [Full Text]  
  • Dangoor, I., Peled-Zehavi, H., Levitan, A., Pasand, O., Danon, A. (2009). A Small Family of Chloroplast Atypical Thioredoxins. Plant Physiol. 149: 1240-1250 [Abstract] [Full Text]  
  • Balsera, M., Goetze, T. A., Kovacs-Bogdan, E., Schurmann, P., Wagner, R., Buchanan, B. B., Soll, J., Bolter, B. (2009). Characterization of Tic110, a Channel-forming Protein at the Inner Envelope Membrane of Chloroplasts, Unveils a Response to Ca2+ and a Stromal Regulatory Disulfide Bridge. J. Biol. Chem. 284: 2603-2616 [Abstract] [Full Text]  
  • Uniacke, J., Zerges, W. (2008). Stress induces the assembly of RNA granules in the chloroplast of Chlamydomonas reinhardtii. JCB 182: 641-646 [Abstract] [Full Text]  
  • Michelet, L., Zaffagnini, M., Vanacker, H., Le Marechal, P., Marchand, C., Schroda, M., Lemaire, S. D., Decottignies, P. (2008). In Vivo Targets of S-Thiolation in Chlamydomonas reinhardtii. J. Biol. Chem. 283: 21571-21578 [Abstract] [Full Text]  
  • Stengel, A., Benz, P., Balsera, M., Soll, J., Bolter, B. (2008). TIC62 Redox-regulated Translocon Composition and Dynamics. J. Biol. Chem. 283: 6656-6667 [Abstract] [Full Text]  
  • Lefebvre-Legendre, L., Rappaport, F., Finazzi, G., Ceol, M., Grivet, C., Hopfgartner, G., Rochaix, J.-D. (2007). Loss of Phylloquinone in Chlamydomonas Affects Plastoquinone Pool Size and Photosystem II Synthesis. J. Biol. Chem. 282: 13250-13263 [Abstract] [Full Text]  
  • Schult, K., Meierhoff, K., Paradies, S., Toller, T., Wolff, P., Westhoff, P. (2007). The Nuclear-Encoded Factor HCF173 Is Involved in the Initiation of Translation of the psbA mRNA in Arabidopsis thaliana. Plant Cell 19: 1329-1346 [Abstract] [Full Text]  
  • Alergand, T., Peled-Zehavi, H., Katz, Y., Danon, A. (2006). The Chloroplast Protein Disulfide Isomerase RB60 Reacts with a Regulatory Disulfide of the RNA-binding Protein RB47. Plant Cell Physiol 47: 540-548 [Abstract] [Full Text]  
  • Levitan, A., Trebitsh, T., Kiss, V., Pereg, Y., Dangoor, I., Danon, A. (2005). Dual targeting of the protein disulfide isomerase RB60 to the chloroplast and the endoplasmic reticulum. Proc. Natl. Acad. Sci. USA 102: 6225-6230 [Abstract] [Full Text]  
  • Yosef, I., Irihimovitch, V., Knopf, J. A., Cohen, I., Orr-Dahan, I., Nahum, E., Keasar, C., Shapira, M. (2004). RNA Binding Activity of the Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large Subunit from Chlamydomonas reinhardtii. J. Biol. Chem. 279: 10148-10156 [Abstract] [Full Text]  
  • Lilly, J. W., Maul, J. E., Stern, D. B. (2002). The Chlamydomonas reinhardtii Organellar Genomes Respond Transcriptionally and Post-Transcriptionally to Abiotic Stimuli. Plant Cell 14: 2681-2706 [Abstract] [Full Text]  
  • Auchincloss, A. H., Zerges, W., Perron, K., Girard-Bascou, J., Rochaix, J.-D. (2002). Characterization of Tbc2, a nucleus-encoded factor specifically required for translation of the chloroplast psbC mRNA in Chlamydomonas reinhardtii. JCB 157: 953-962 [Abstract] [Full Text]  
  • Katz, Y. S., Danon, A. (2002). The 3'-Untranslated Region of Chloroplast psbA mRNA Stabilizes Binding of Regulatory Proteins to the Leader of the Message. J. Biol. Chem. 277: 18665-18669 [Abstract] [Full Text]  
  • Vranova, E., Inze, D., Van Breusegem, F. (2002). Signal transduction during oxidative stress. J Exp Bot 53: 1227-1236 [Abstract] [Full Text]  
  • Hager, M., Hermann, M., Biehler, K., Krieger-Liszkay, A., Bock, R. (2002). Lack of the Small Plastid-encoded PsbJ Polypeptide Results in a Defective Water-splitting Apparatus of Photosystem II, Reduced Photosystem I Levels, and Hypersensitivity to Light. J. Biol. Chem. 277: 14031-14039 [Abstract] [Full Text]  
  • Baena-Gonzalez, E., Baginsky, S., Mulo, P., Summer, H., Aro, E.-M., Link, G. (2001). Chloroplast Transcription at Different Light Intensities. Glutathione-Mediated Phosphorylation of the Major RNA Polymerase Involved in Redox-Regulated Organellar Gene Expression. Plant Physiol. 127: 1044-1052 [Abstract] [Full Text]  
  • Shen, Y., Danon, A., Christopher, D. A. (2001). RNA Binding-Proteins Interact Specifically with the Arabidopsis Chloroplast psbA mRNA 5' Untranslated Region in a Redox-Dependent Manner. Plant Cell Physiol 42: 1071-1078 [Abstract] [Full Text]  
  • Trebitsh, T., Danon, A. (2001). Translation of chloroplast psbA mRNA is regulated by signals initiated by both photosystems II and I. Proc. Natl. Acad. Sci. USA 10.1073/pnas.211440698v1 [Abstract] [Full Text]  
  • Shteiman-Kotler, A., Schuster, G. (2000). RNA-binding characteristics of the chloroplast S1-like ribosomal protein CS1. Nucleic Acids Res 28: 3310-3315 [Abstract] [Full Text]  
  • Trebitsh, T., Meiri, E., Ostersetzer, O., Adam, Z., Danon, A. (2001). The Protein Disulfide Isomerase-like RB60 Is Partitioned between Stroma and Thylakoids in Chlamydomonas reinhardtii Chloroplasts. J. Biol. Chem. 276: 4564-4569 [Abstract] [Full Text]  
  • McCormac, D. J., Litz, H., Wang, J., Gollnick, P. D., Berry, J. O. (2001). Light-associated and Processing-dependent Protein Binding to 5' Regions of rbcL mRNA in the Chloroplasts of a C4 Plant. J. Biol. Chem. 276: 3476-3483 [Abstract] [Full Text]  
  • Trebitsh, T., Danon, A. (2001). Translation of chloroplast psbA mRNA is regulated by signals initiated by both photosystems II and I. Proc. Natl. Acad. Sci. USA 98: 12289-12294 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»