Transient Expression of Cellular Polypyrimidine-Tract Binding Protein Stimulates Cap-Independent Translation Directed by Both Picornaviral and Flaviviral Internal Ribosome Entry Sites In Vivo

doi:10.1128/MCB.20.5.1583-1595.2000

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Molecular and Cellular Biology, March 2000, p. 1583-1595, Vol. 20, No. 5
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Transient Expression of Cellular Polypyrimidine-Tract Binding Protein Stimulates Cap-Independent Translation Directed by Both Picornaviral and Flaviviral Internal Ribosome Entry Sites In Vivo

Rainer Gosert,¹ Ki Ha Chang,¹^, Rene Rijnbrand,² MinKyung Yi,² David V. Sangar,² and Stanley M. Lemon¹^,²^,^*

Department of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7030,¹ and Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas 77555-1019²

Received 23 August 1999/Returned for modification 29 October 1999/Accepted 30 November 1999

The regulation of cap-independent translation directed by the internal ribosome entry sites (IRESs) present in some viraland cellular RNAs is poorly understood. Polypyrimidine-tract bindingprotein (PTB) binds specifically to several viral IRESs. IRES-directedtranslation may be reduced in cell-free systems that are depletedof PTB and restored by reconstitution of lysates with recombinantPTB. However, there are no data concerning the effects of PTBon IRES-directed translation in vivo. We transfected cells withplasmids expressing dicistronic transcripts in which the upstreamcistron encoded PTB or PTB deletion mutants (including a nullmutant lacking amino acid residues 87 to 531). The downstreamcistron encoded a reporter protein (chloramphenicol acetyltransferase[CAT]) under translational control of the poliovirus IRES whichwas placed within the intercistronic space. In transfected BS-C-1cells, transcripts expressing wild-type PTB produced 12-fold morereporter protein than similar transcripts encoding the PTB nullmutant. There was a 2.4-fold difference in CAT produced from thesetranscripts in HeLa cells, which contain a greater natural abundanceof PTB. PTB similarly stimulated CAT production from transcriptscontaining the IRES of hepatitis A virus or hepatitis C virusin BS-C-1 cells and Huh-7 cells (37- to 44-fold increase and 5to 5.3-fold increase, respectively). Since PTB had no quantitativeor qualitative effect on transcription from these plasmids, weconclude that PTB stimulates translation of representative picornaviraland flaviviral RNAs in vivo. This is likely to reflect the stabilizationof higher ordered RNA structures within the IRES and was not observedwith PTB mutants lacking RNA recognition motifs located in theC-terminal third of themolecule.

^* Corresponding author. Mailing address: Department of Microbiology and Immunology, The University of Texas Medical Branch,301 University Blvd., Galveston, Texas 77555-1019. Phone: (409)772-2324. Fax: (409) 772-3757. E-mail: smlemon{at}utmb.edu.

Deceased.

Molecular and Cellular Biology, March 2000, p. 1583-1595, Vol. 20, No. 5
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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