This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wallberg, A. E.
Right arrow Articles by Wright, A. P. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wallberg, A. E.
Right arrow Articles by Wright, A. P. H.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, March 2000, p. 2004-2013, Vol. 20, No. 6
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Recruitment of the SWI-SNF Chromatin Remodeling Complex as a Mechanism of Gene Activation by the Glucocorticoid Receptor tau 1 Activation Domain

Annika E. Wallberg,1,* Kristen E. Neely,2 Ahmed H. Hassan,2 Jan-Åke Gustafsson,1 Jerry L. Workman,2 and Anthony P. H. Wright3

Karolinska Institute, Department of Biosciences, NOVUM, S-14157 Huddinge,1 and Södertörns högskola, S-14104 Huddinge,3 Sweden, and Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802-45002

Received 9 August 1999/Returned for modification 14 September 1999/Accepted 20 December 1999

The SWI-SNF complex has been shown to alter nucleosome conformation in an ATP-dependent manner, leading to increased accessibility of nucleosomal DNA to transcription factors. In this study, we show that the SWI-SNF complex can potentiate the activity of the glucocorticoid receptor (GR) through the N-terminal transactivation domain, tau 1, in both yeast and mammalian cells. GR-tau 1 can directly interact with purified SWI-SNF complex, and mutations in tau 1 that affect the transactivation activity in vivo also directly affect tau 1 interaction with SWI-SNF. Furthermore, the SWI-SNF complex can stimulate tau 1-driven transcription from chromatin templates in vitro. Taken together, these results support a model in which the GR can directly recruit the SWI-SNF complex to target promoters during glucocorticoid-dependent gene activation. We also provide evidence that the SWI-SNF and SAGA complexes represent independent pathways of tau 1-mediated activation but play overlapping roles that are able to compensate for one another under some conditions.


* Corresponding author. Mailing address: The Rockefeller University, Box 166, c/o Roeder Lab, 1230 York Ave., New York, NY 10021. Phone: (212) 327-7604. Fax: (212) 327-7949. E-mail: wallbea{at}rockvax.rockefeller.edu.


Molecular and Cellular Biology, March 2000, p. 2004-2013, Vol. 20, No. 6
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Lee, S., Kim, D.-H., Goo, Y. H., Lee, Y. C., Lee, S.-K., Lee, J. W. (2009). Crucial Roles for Interactions between MLL3/4 and INI1 in Nuclear Receptor Transactivation. Mol. Endocrinol. 23: 610-619 [Abstract] [Full Text]  
  • Pottier, N., Yang, W., Assem, M., Panetta, J. C., Pei, D., Paugh, S. W., Cheng, C., Den Boer, M. L., Relling, M. V., Pieters, R., Evans, W. E., Cheok, M. H. (2008). The SWI/SNF Chromatin-Remodeling Complex and Glucocorticoid Resistance in Acute Lymphoblastic Leukemia. JNCI J Natl Cancer Inst 100: 1792-1803 [Abstract] [Full Text]  
  • Dai, Y., Ngo, D., Jacob, J., Forman, L. W., Faller, D. V. (2008). Prohibitin and the SWI/SNF ATPase subunit BRG1 are required for effective androgen antagonist-mediated transcriptional repression of androgen receptor-regulated genes. Carcinogenesis 29: 1725-1733 [Abstract] [Full Text]  
  • Heimeier, R. A., Hsia, V. S., Shi, Y.-B. (2008). Participation of Brahma-Related Gene 1 (BRG1)-Associated Factor 57 and BRG1-Containing Chromatin Remodeling Complexes in Thyroid Hormone-Dependent Gene Activation during Vertebrate Development. Mol. Endocrinol. 22: 1065-1077 [Abstract] [Full Text]  
  • Kim, D.-H., Kim, G. S., Yun, C. H., Lee, Y. C. (2008). Functional Conservation of the Glutamine-Rich Domains of Yeast Gal11 and Human SRC-1 in the Transactivation of Glucocorticoid Receptor Tau 1 in Saccharomyces cerevisiae. Mol. Cell. Biol. 28: 913-925 [Abstract] [Full Text]  
  • Pottier, N., Cheok, M. H., Yang, W., Assem, M., Tracey, L., Obenauer, J. C., Panetta, J. C., Relling, M. V., Evans, W. E. (2007). Expression of SMARCB1 modulates steroid sensitivity in human lymphoblastoid cells: identification of a promoter snp that alters PARP1 binding and SMARCB1 expression. Hum Mol Genet 16: 2261-2271 [Abstract] [Full Text]  
  • Hassan, A. H., Awad, S., Prochasson, P. (2006). The Swi2/Snf2 Bromodomain Is Required for the Displacement of SAGA and the Octamer Transfer of SAGA-acetylated Nucleosomes. J. Biol. Chem. 281: 18126-18134 [Abstract] [Full Text]  
  • Perani, M., Antonson, P., Hamoudi, R., Ingram, C. J. E., Cooper, C. S., Garrett, M. D., Goodwin, G. H. (2005). The Proto-oncoprotein SYT Interacts with SYT-interacting Protein/Co-activator Activator (SIP/CoAA), a Human Nuclear Receptor Co-activator with Similarity to EWS and TLS/FUS Family of Proteins. J. Biol. Chem. 280: 42863-42876 [Abstract] [Full Text]  
  • Inayoshi, Y., Kaneoka, H., Machida, Y., Terajima, M., Dohda, T., Miyake, K., Iijima, S. (2005). Repression of GR-Mediated Expression of the Tryptophan Oxygenase Gene by the SWI/SNF Complex during Liver Development. J Biochem 138: 457-465 [Abstract] [Full Text]  
  • Dhasarathy, A., Kladde, M. P. (2005). Promoter Occupancy Is a Major Determinant of Chromatin Remodeling Enzyme Requirements. Mol. Cell. Biol. 25: 2698-2707 [Abstract] [Full Text]  
  • Kim, T. S., Kim, H. Y., Yoon, J. H., Kang, H. S. (2004). Recruitment of the Swi/Snf Complex by Ste12-Tec1 Promotes Flo8-Mss11-Mediated Activation of STA1 Expression. Mol. Cell. Biol. 24: 9542-9556 [Abstract] [Full Text]  
  • Angelov, D., Lenouvel, F., Hans, F., Muller, C. W., Bouvet, P., Bednar, J., Moudrianakis, E. N., Cadet, J., Dimitrov, S. (2004). The Histone Octamer Is Invisible When NF-{kappa}B Binds to the Nucleosome. J. Biol. Chem. 279: 42374-42382 [Abstract] [Full Text]  
  • Ko, M., Jang, J., Ahn, J., Lee, K., Chung, H., Jeon, S. H., Seong, R. H. (2004). T Cell Receptor Signaling Inhibits Glucocorticoid-induced Apoptosis by Repressing the SRG3 Expression via Ras Activation. J. Biol. Chem. 279: 21903-21915 [Abstract] [Full Text]  
  • Debril, M.-B., Gelman, L., Fayard, E., Annicotte, J.-S., Rocchi, S., Auwerx, J. (2004). Transcription Factors and Nuclear Receptors Interact with the SWI/SNF Complex through the BAF60c Subunit. J. Biol. Chem. 279: 16677-16686 [Abstract] [Full Text]  
  • Trotter, K. W., Archer, T. K. (2004). Reconstitution of Glucocorticoid Receptor-Dependent Transcription In Vivo. Mol. Cell. Biol. 24: 3347-3358 [Abstract] [Full Text]  
  • Rehtanz, M., Schmidt, H.-M., Warthorst, U., Steger, G. (2004). Direct Interaction between Nucleosome Assembly Protein 1 and the Papillomavirus E2 Proteins Involved in Activation of Transcription. Mol. Cell. Biol. 24: 2153-2168 [Abstract] [Full Text]  
  • Rogatsky, I., Wang, J.-C., Derynck, M. K., Nonaka, D. F., Khodabakhsh, D. B., Haqq, C. M., Darimont, B. D., Garabedian, M. J., Yamamoto, K. R. (2003). Target-specific utilization of transcriptional regulatory surfaces by the glucocorticoid receptor. Proc. Natl. Acad. Sci. USA 100: 13845-13850 [Abstract] [Full Text]  
  • Kennedy, G., Sugden, B. (2003). EBNA-1, a Bifunctional Transcriptional Activator. Mol. Cell. Biol. 23: 6901-6908 [Abstract] [Full Text]  
  • Valerius, O., Brendel, C., Wagner, C., Krappmann, S., Thoma, F., Braus, G. H. (2003). Nucleosome Position-Dependent and -Independent Activation of HIS7 Expression in Saccharomyces cerevisiae by Different Transcriptional Activators. Eukaryot Cell 2: 876-885 [Abstract] [Full Text]  
  • Hsiao, P.-W., Fryer, C. J., Trotter, K. W., Wang, W., Archer, T. K. (2003). BAF60a Mediates Critical Interactions between Nuclear Receptors and the BRG1 Chromatin-Remodeling Complex for Transactivation. Mol. Cell. Biol. 23: 6210-6220 [Abstract] [Full Text]  
  • Marshall, T. W., Link, K. A., Petre-Draviam, C. E., Knudsen, K. E. (2003). Differential Requirement of SWI/SNF for Androgen Receptor Activity. J. Biol. Chem. 278: 30605-30613 [Abstract] [Full Text]  
  • Mittelstadt, P. R., Ashwell, J. D. (2003). Disruption of Glucocorticoid Receptor Exon 2 Yields a Ligand-Responsive C-Terminal Fragment that Regulates Gene Expression. Mol. Endocrinol. 17: 1534-1542 [Abstract] [Full Text]  
  • Gwack, Y., Baek, H. J., Nakamura, H., Lee, S. H., Meisterernst, M., Roeder, R. G., Jung, J. U. (2003). Principal Role of TRAP/Mediator and SWI/SNF Complexes in Kaposi's Sarcoma-Associated Herpesvirus RTA-Mediated Lytic Reactivation. Mol. Cell. Biol. 23: 2055-2067 [Abstract] [Full Text]  
  • Inoue, H., Furukawa, T., Giannakopoulos, S., Zhou, S., King, D. S., Tanese, N. (2002). Largest Subunits of the Human SWI/SNF Chromatin-remodeling Complex Promote Transcriptional Activation by Steroid Hormone Receptors. J. Biol. Chem. 277: 41674-41685 [Abstract] [Full Text]  
  • Mudhasani, R., Fontes, J. D. (2002). The Class II Transactivator Requires brahma-Related Gene 1 To Activate Transcription of Major Histocompatibility Complex Class II Genes. Mol. Cell. Biol. 22: 5019-5026 [Abstract] [Full Text]  
  • Flinn, E. M., Wallberg, A. E., Hermann, S., Grant, P. A., Workman, J. L., Wright, A. P. H. (2002). Recruitment of Gcn5-containing Complexes during c-Myc-dependent Gene Activation. STRUCTURE AND FUNCTION ASPECTS. J. Biol. Chem. 277: 23399-23406 [Abstract] [Full Text]  
  • Lee, D., Kim, J. W., Seo, T., Hwang, S. G., Choi, E.-J., Choe, J. (2002). SWI/SNF Complex Interacts with Tumor Suppressor p53 and Is Necessary for the Activation of p53-mediated Transcription. J. Biol. Chem. 277: 22330-22337 [Abstract] [Full Text]  
  • Rouleau, N., Domans'kyi, A., Reeben, M., Moilanen, A.-M., Havas, K., Kang, Z., Owen-Hughes, T., Palvimo, J. J., Janne, O. A. (2002). Novel ATPase of SNF2-like Protein Family Interacts with Androgen Receptor and Modulates Androgen-dependent Transcription. Mol. Biol. Cell 13: 2106-2119 [Abstract] [Full Text]  
  • Fletcher, T. M., Xiao, N., Mautino, G., Baumann, C. T., Wolford, R., Warren, B. S., Hager, G. L. (2002). ATP-Dependent Mobilization of the Glucocorticoid Receptor during Chromatin Remodeling. Mol. Cell. Biol. 22: 3255-3263 [Abstract] [Full Text]  
  • Lu, R., Moore, P. A., Pitha, P. M. (2002). Stimulation of IRF-7 Gene Expression by Tumor Necrosis Factor alpha . REQUIREMENT FOR NFkappa B TRANSCRIPTION FACTOR AND GENE ACCESSIBILITY. J. Biol. Chem. 277: 16592-16598 [Abstract] [Full Text]  
  • Wang, S., Hankinson, O. (2002). Functional Involvement of the Brahma/SWI2-related Gene 1 Protein in Cytochrome P4501A1 Transcription Mediated by the Aryl Hydrocarbon Receptor Complex. J. Biol. Chem. 277: 11821-11827 [Abstract] [Full Text]  
  • Neely, K. E., Hassan, A. H., Brown, C. E., Howe, L., Workman, J. L. (2002). Transcription Activator Interactions with Multiple SWI/SNF Subunits. Mol. Cell. Biol. 22: 1615-1625 [Abstract] [Full Text]  
  • Kato, H., Tjernberg, A., Zhang, W., Krutchinsky, A. N., An, W., Takeuchi, T., Ohtsuki, Y., Sugano, S., de Bruijn, D. R., Chait, B. T., Roeder, R. G. (2002). SYT Associates with Human SNF/SWI Complexes and the C-terminal Region of Its Fusion Partner SSX1 Targets Histones. J. Biol. Chem. 277: 5498-5505 [Abstract] [Full Text]  
  • Bodmer-Glavas, M., Edler, K., Barberis, A. (2001). RNA polymerase II and III transcription factors can stimulate DNA replication by modifying origin chromatin structures. Nucleic Acids Res 29: 4570-4580 [Abstract] [Full Text]  
  • Rosinski-Chupin, I., Huaulme, J.-F., Rougeot, C., Rougeon, F. (2001). The Transcriptional Response to Androgens of the Rat VCSA1 Gene Is Amplified by Both Binary and Graded Mechanisms. Endocrinology 142: 4550-4559 [Abstract] [Full Text]  
  • Han, S., Choi, H., Ko, M.-g., Choi, Y. I., Sohn, D. H., Kim, J. K., Shin, D., Chung, H., Lee, H. W., Kim, J.-B., Park, S. D., Seong, R. H. (2001). Peripheral T Cells Become Sensitive to Glucocorticoid- and Stress-Induced Apoptosis in Transgenic Mice Overexpressing SRG3. J. Immunol. 167: 805-810 [Abstract] [Full Text]  
  • Urnov, F. D., Wolffe, A. P. (2001). A Necessary Good: Nuclear Hormone Receptors and Their Chromatin Templates. Mol. Endocrinol. 15: 1-16 [Full Text]  
  • Nie, Z., Xue, Y., Yang, D., Zhou, S., Deroo, B. J., Archer, T. K., Wang, W. (2000). A Specificity and Targeting Subunit of a Human SWI/SNF Family-Related Chromatin-Remodeling Complex. Mol. Cell. Biol. 20: 8879-8888 [Abstract] [Full Text]  
  • Wu, D. Y., Krumm, A., Schubach, W. H. (2000). Promoter-Specific Targeting of Human SWI-SNF Complex by Epstein-Barr Virus Nuclear Protein 2. J. Virol. 74: 8893-8903 [Abstract] [Full Text]  
  • Kadam, S., McAlpine, G. S., Phelan, M. L., Kingston, R. E., Jones, K. A., Emerson, B. M. (2000). Functional selectivity of recombinant mammalian SWI/SNF subunits. Genes Dev. 14: 2441-2451 [Abstract] [Full Text]