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Molecular and Cellular Biology, March 2000, p. 2004-2013, Vol. 20, No. 6
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Recruitment of the SWI-SNF Chromatin Remodeling Complex as a Mechanism of Gene Activation by the Glucocorticoid Receptor tau 1 Activation Domain

Annika E. Wallberg,1,* Kristen E. Neely,2 Ahmed H. Hassan,2 Jan-Åke Gustafsson,1 Jerry L. Workman,2 and Anthony P. H. Wright3

Karolinska Institute, Department of Biosciences, NOVUM, S-14157 Huddinge,1 and Södertörns högskola, S-14104 Huddinge,3 Sweden, and Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802-45002

Received 9 August 1999/Returned for modification 14 September 1999/Accepted 20 December 1999

The SWI-SNF complex has been shown to alter nucleosome conformation in an ATP-dependent manner, leading to increased accessibility of nucleosomal DNA to transcription factors. In this study, we show that the SWI-SNF complex can potentiate the activity of the glucocorticoid receptor (GR) through the N-terminal transactivation domain, tau 1, in both yeast and mammalian cells. GR-tau 1 can directly interact with purified SWI-SNF complex, and mutations in tau 1 that affect the transactivation activity in vivo also directly affect tau 1 interaction with SWI-SNF. Furthermore, the SWI-SNF complex can stimulate tau 1-driven transcription from chromatin templates in vitro. Taken together, these results support a model in which the GR can directly recruit the SWI-SNF complex to target promoters during glucocorticoid-dependent gene activation. We also provide evidence that the SWI-SNF and SAGA complexes represent independent pathways of tau 1-mediated activation but play overlapping roles that are able to compensate for one another under some conditions.

* Corresponding author. Mailing address: The Rockefeller University, Box 166, c/o Roeder Lab, 1230 York Ave., New York, NY 10021. Phone: (212) 327-7604. Fax: (212) 327-7949. E-mail: wallbea{at}rockvax.rockefeller.edu.

Molecular and Cellular Biology, March 2000, p. 2004-2013, Vol. 20, No. 6
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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