This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhou, S.
Right arrow Articles by Hayward, S. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhou, S.
Right arrow Articles by Hayward, S. D.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2000, p. 2400-2410, Vol. 20, No. 7
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

SKIP, a CBF1-Associated Protein, Interacts with the Ankyrin Repeat Domain of NotchIC To Facilitate NotchIC Function

Sifang Zhou,1 Masahiro Fujimuro,1 James J.-D. Hsieh,1 Lin Chen,1 Alison Miyamoto,2 Gerry Weinmaster,2 and S. Diane Hayward1,3,*

Department of Pharmacology and Molecular Sciences1 and Oncology Center,3 Johns Hopkins School of Medicine, Baltimore, Maryland 21205, and Molecular Biology Institute, University of California, Los Angeles, California 900952

Received 18 October 1999/Returned for modification 13 December 1999/Accepted 12 January 2000

Notch proteins are transmembrane receptors that mediate intercell communication and direct individual cell fate decisions. The activated intracellular form of Notch, NotchIC, translocates to the nucleus, where it targets the DNA binding protein CBF1. CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. We have examined the mechanism whereby NotchIC overcomes CBF1-mediated transcriptional repression. We identified SKIP (Ski-interacting protein) as a CBF1 binding protein in a yeast two-hybrid screen. Both CBF1 and SKIP are highly conserved evolutionarily, and the SKIP-CBF1 interaction is also conserved in assays using the Caenorhabditis elegans and Drosophila melanogaster SKIP homologs. Protein-protein interaction assays demonstrated interaction between SKIP and the corepressor SMRT. More surprisingly, SKIP also interacted with NotchIC. The SMRT and NotchIC interactions were mutually exclusive. In competition binding experiments SMRT displaced NotchIC from CBF1 and from SKIP. Contact with SKIP is required for biological activity of NotchIC. A mutation in the fourth ankyrin repeat that abolished Notch signal transduction did not affect interaction with CBF1 but abolished interaction with SKIP. Further, NotchIC was unable to block muscle cell differentiation in myoblasts expressing antisense SKIP. The results suggest a model in which NotchIC activates responsive promoters by competing with the SMRT-corepressor complex for contacts on both CBF1 and SKIP.

* Corresponding author. Mailing address: Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, 725 N. Wolfe St., Baltimore, MD 21205. Phone: (410) 955-2548. Fax: (410) 955-8685. E-mail: dhayward{at}jhmi.edu.

Molecular and Cellular Biology, April 2000, p. 2400-2410, Vol. 20, No. 7
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Huang, L., Chi, J., Berry, F. B., Footz, T. K., Sharp, M. W., Walter, M. A. (2008). Human p32 Is a Novel FOXC1-Interacting Protein That Regulates FOXC1 Transcriptional Activity in Ocular Cells. IOVS 49: 5243-5249 [Abstract] [Full Text]  
  • del Alamo, D., Mlodzik, M. (2008). Self-modulation of Notch signaling during ommatidial development via the Roughened eye transcriptional repressor. Development 135: 2895-2904 [Abstract] [Full Text]  
  • Friedmann, D. R., Wilson, J. J., Kovall, R. A. (2008). RAM-induced Allostery Facilitates Assembly of a Notch Pathway Active Transcription Complex. J. Biol. Chem. 283: 14781-14791 [Abstract] [Full Text]  
  • Sakamoto, K., Tamamura, Y., Katsube, K.-i., Yamaguchi, A. (2008). Zfp64 participates in Notch signaling and regulates differentiation in mesenchymal cells. J. Cell Sci. 121: 1613-1623 [Abstract] [Full Text]  
  • Michie, A. M., Chan, A. C., Ciofani, M., Carleton, M., Lefebvre, J. M., He, Y., Allman, D. M., Wiest, D. L., Zuniga-Pflucker, J. C., Izon, D. J. (2007). Constitutive Notch signalling promotes CD4 CD8 thymocyte differentiation in the absence of the pre-TCR complex, by mimicking pre-TCR signals. Int Immunol 19: 1421-1430 [Abstract] [Full Text]  
  • Fiuza, U.-M., Arias, A. M. (2007). Cell and molecular biology of Notch. J Endocrinol 194: 459-474 [Abstract] [Full Text]  
  • Niessen, K., Karsan, A. (2007). Notch signaling in the developing cardiovascular system. Am. J. Physiol. Cell Physiol. 293: C1-C11 [Abstract] [Full Text]  
  • Curry, C. L., Reed, L. L., Broude, E., Golde, T. E., Miele, L., Foreman, K. E. (2007). Notch inhibition in Kaposi's sarcoma tumor cells leads to mitotic catastrophe through nuclear factor-{kappa}B signaling. Molecular Cancer Therapeutics 6: 1983-1992 [Abstract] [Full Text]  
  • Ma, X., Renda, M. J., Wang, L., Cheng, E.-c., Niu, C., Morris, S. W., Chi, A. S., Krause, D. S. (2007). Rbm15 Modulates Notch-Induced Transcriptional Activation and Affects Myeloid Differentiation. Mol. Cell. Biol. 27: 3056-3064 [Abstract] [Full Text]  
  • Ehebauer, M., Hayward, P., Martinez-Arias, A. (2006). Notch Signaling Pathway. Sci Signal 2006: cm7-cm7 [Abstract] [Full Text]  
  • Carroll, K. D., Bu, W., Palmeri, D., Spadavecchia, S., Lynch, S. J., Marras, S. A. E., Tyagi, S., Lukac, D. M. (2006). Kaposi's Sarcoma-Associated Herpesvirus Lytic Switch Protein Stimulates DNA Binding of RBP-Jk/CSL To Activate the Notch Pathway. J. Virol. 80: 9697-9709 [Abstract] [Full Text]  
  • Nagel, A. C., Krejci, A., Tenin, G., Bravo-Patino, A., Bray, S., Maier, D., Preiss, A. (2005). Hairless-Mediated Repression of Notch Target Genes Requires the Combined Activity of Groucho and CtBP Corepressors. Mol. Cell. Biol. 25: 10433-10441 [Abstract] [Full Text]  
  • Katic, I., Vallier, L. G., Greenwald, I. (2005). New Positive Regulators of lin-12 Activity in Caenorhabditis elegans Include the BRE-5/Brainiac Glycosphingolipid Biosynthesis Enzyme. Genetics 171: 1605-1615 [Abstract] [Full Text]  
  • Maier, S., Santak, M., Mantik, A., Grabusic, K., Kremmer, E., Hammerschmidt, W., Kempkes, B. (2005). A Somatic Knockout of CBF1 in a Human B-Cell Line Reveals that Induction of CD21 and CCR7 by EBNA-2 Is Strictly CBF1 Dependent and that Downregulation of Immunoglobulin M Is Partially CBF1 Independent. J. Virol. 79: 8784-8792 [Abstract] [Full Text]  
  • Wu, J., Iwata, F., Grass, J. A., Osborne, C. S., Elnitski, L., Fraser, P., Ohneda, O., Yamamoto, M., Bresnick, E. H. (2005). Molecular Determinants of NOTCH4 Transcription in Vascular Endothelium. Mol. Cell. Biol. 25: 1458-1474 [Abstract] [Full Text]  
  • Chen, H., Huang, J., Wu, F. Y., Liao, G., Hutt-Fletcher, L., Hayward, S. D. (2005). Regulation of Expression of the Epstein-Barr Virus BamHI-A Rightward Transcripts. J. Virol. 79: 1724-1733 [Abstract] [Full Text]  
  • Lee, J. M., Lee, K.-H., Farrell, C. J., Ling, P. D., Kempkes, B., Park, J. H., Hayward, S. D. (2004). EBNA2 Is Required for Protection of Latently Epstein-Barr Virus-Infected B Cells against Specific Apoptotic Stimuli. J. Virol. 78: 12694-12697 [Abstract] [Full Text]  
  • Laduron, S., Deplus, R., Zhou, S., Kholmanskikh, O., Godelaine, D., De Smet, C., Hayward, S. D., Fuks, F., Boon, T., De Plaen, E. (2004). MAGE-A1 interacts with adaptor SKIP and the deacetylase HDAC1 to repress transcription. Nucleic Acids Res 32: 4340-4350 [Abstract] [Full Text]  
  • Tanimizu, N., Miyajima, A. (2004). Notch signaling controls hepatoblast differentiation by altering the expression of liver-enriched transcription factors. J. Cell Sci. 117: 3165-3174 [Abstract] [Full Text]  
  • Ross, D. A., Rao, P. K., Kadesch, T. (2004). Dual Roles for the Notch Target Gene Hes-1 in the Differentiation of 3T3-L1 Preadipocytes. Mol. Cell. Biol. 24: 3505-3513 [Abstract] [Full Text]  
  • Trifonova, R., Small, D., Kacer, D., Kovalenko, D., Kolev, V., Mandinova, A., Soldi, R., Liaw, L., Prudovsky, I., Maciag, T. (2004). The non-transmembrane form of Delta1 but not of jagged1 Induces normal migratory behavior accompanied by FGF receptor 1-dependent transformation. J. Biol. Chem. 0: 300564200-1 [Abstract]  
  • Chu, J., Bresnick, E. H. (2004). Evidence That C Promoter-binding Factor 1 Binding Is Required for Notch-1-mediated Repression of Activator Protein-1. J. Biol. Chem. 279: 12337-12345 [Abstract] [Full Text]  
  • Dahlqvist, C., Blokzijl, A., Chapman, G., Falk, A., Dannaeus, K., Ibanez, C. F., Lendahl, U. (2003). Functional Notch signaling is required for BMP4-induced inhibition of myogenic differentiation. Development 130: 6089-6099 [Abstract] [Full Text]  
  • Sciaudone, M., Gazzerro, E., Priest, L., Delany, A. M., Canalis, E. (2003). Notch 1 Impairs Osteoblastic Cell Differentiation. Endocrinology 144: 5631-5639 [Abstract] [Full Text]  
  • Yeh, T.-S., Lin, Y.-M., Hsieh, R.-H., Tseng, M.-J. (2003). Association of Transcription Factor YY1 with the High Molecular Weight Notch Complex Suppresses the Transactivation Activity of Notch. J. Biol. Chem. 278: 41963-41969 [Abstract] [Full Text]  
  • Alazard, N., Gruffat, H., Hiriart, E., Sergeant, A., Manet, E. (2003). Differential Hyperacetylation of Histones H3 and H4 upon Promoter-Specific Recruitment of EBNA2 in Epstein-Barr Virus Chromatin. J. Virol. 77: 8166-8172 [Abstract] [Full Text]  
  • Oakley, F., Mann, J., Ruddell, R. G., Pickford, J., Weinmaster, G., Mann, D. A. (2003). Basal Expression of I{kappa}B{alpha} Is Controlled by the Mammalian Transcriptional Repressor RBP-J (CBF1) and Its Activator Notch1. J. Biol. Chem. 278: 24359-24370 [Abstract] [Full Text]  
  • Kostrouchova, M., Kostrouch, Z., Saudek, V., Piatigorsky, J., Rall, J. E. (2003). BIR-1, a Caenorhabditis elegans homologue of Survivin, regulates transcription and development. Proc. Natl. Acad. Sci. USA 100: 5240-5245 [Abstract] [Full Text]  
  • Barry, J. B., Leong, G. M., Church, W. B., Issa, L. L., Eisman, J. A., Gardiner, E. M. (2003). Interactions of SKIP/NCoA-62, TFIIB, and Retinoid X Receptor with Vitamin D Receptor Helix H10 Residues. J. Biol. Chem. 278: 8224-8228 [Abstract] [Full Text]  
  • Wu, F. Y., Chen, H., Wang, S. E., apRhys, C. M. J., Liao, G., Fujimuro, M., Farrell, C. J., Huang, J., Hayward, S. D., Hayward, G. S. (2002). CCAAT/Enhancer Binding Protein {alpha} Interacts with ZTA and Mediates ZTA-Induced p21CIP-1 Accumulation and G1 Cell Cycle Arrest during the Epstein-Barr Virus Lytic Cycle. J. Virol. 77: 1481-1500 [Abstract] [Full Text]  
  • Prathapam, T., Kuhne, C., Banks, L. (2002). Skip interacts with the retinoblastoma tumor suppressor and inhibits its transcriptional repression activity. Nucleic Acids Res 30: 5261-5268 [Abstract] [Full Text]  
  • Jarriault, S., Greenwald, I. (2002). Suppressors of the egg-laying defective phenotype of sel-12 presenilin mutants implicate the CoREST corepressor complex in LIN-12/Notch signaling in C. elegans. Genes Dev. 16: 2713-2728 [Abstract] [Full Text]  
  • Lee, J. M., Lee, K.-H., Weidner, M., Osborne, B. A., Hayward, S. D. (2002). Epstein-Barr virus EBNA2 blocks Nur77- mediated apoptosis. Proc. Natl. Acad. Sci. USA 99: 11878-11883 [Abstract] [Full Text]  
  • Wu, F. Y., Tang, Q.-Q., Chen, H., ApRhys, C., Farrell, C., Chen, J., Fujimuro, M., Lane, M. D., Hayward, G. S. (2002). Lytic replication-associated protein (RAP) encoded by Kaposi sarcoma-associated herpesvirus causes p21CIP-1-mediated G1 cell cycle arrest through CCAAT/enhancer-binding protein-alpha. Proc. Natl. Acad. Sci. USA 99: 10683-10688 [Abstract] [Full Text]  
  • Wardell, S. E., Boonyaratanakornkit, V., Adelman, J. S., Aronheim, A., Edwards, D. P. (2002). Jun Dimerization Protein 2 Functions as a Progesterone Receptor N-Terminal Domain Coactivator. Mol. Cell. Biol. 22: 5451-5466 [Abstract] [Full Text]  
  • Kostrouchova, M., Housa, D., Kostrouch, Z., Saudek, V., Rall, J. E. (2002). SKIP is an indispensable factor for Caenorhabditis elegans development. Proc. Natl. Acad. Sci. USA 99: 9254-9259 [Abstract] [Full Text]  
  • Gordadze, A. V., Poston, D., Ling, P. D. (2002). The EBNA2 Polyproline Region Is Dispensable for Epstein-Barr Virus-Mediated Immortalization Maintenance. J. Virol. 76: 7349-7355 [Abstract] [Full Text]  
  • Fryer, C. J., Lamar, E., Turbachova, I., Kintner, C., Jones, K. A. (2002). Mastermind mediates chromatin-specific transcription and turnover of the Notch enhancer complex. Genes Dev. 16: 1397-1411 [Abstract] [Full Text]  
  • Roncarati, R., Sestan, N., Scheinfeld, M. H., Berechid, B. E., Lopez, P. A., Meucci, O., McGlade, J. C., Rakic, P., D'Adamio, L. (2002). The gamma -secretase-generated intracellular domain of beta -amyloid precursor protein binds Numb and inhibits Notch signaling. Proc. Natl. Acad. Sci. USA 99: 7102-7107 [Abstract] [Full Text]  
  • Sriuranpong, V., Borges, M. W., Strock, C. L., Nakakura, E. K., Watkins, D. N., Blaumueller, C. M., Nelkin, B. D., Ball, D. W. (2002). Notch Signaling Induces Rapid Degradation of Achaete-Scute Homolog 1. Mol. Cell. Biol. 22: 3129-3139 [Abstract] [Full Text]  
  • Chu, J., Jeffries, S., Norton, J. E., Capobianco, A. J., Bresnick, E. H. (2002). Repression of Activator Protein-1-mediated Transcriptional Activation by the Notch-1 Intracellular Domain. J. Biol. Chem. 277: 7587-7597 [Abstract] [Full Text]  
  • Fennewald, S. M., Aronson, J. F., Zhang, L., Herzog, N. K. (2002). Alterations in NF-{kappa}B and RBP-J{kappa} by Arenavirus Infection of Macrophages In Vitro and In Vivo. J. Virol. 76: 1154-1162 [Abstract] [Full Text]  
  • Ross, D. A., Kadesch, T. (2001). The Notch Intracellular Domain Can Function as a Coactivator for LEF-1. Mol. Cell. Biol. 21: 7537-7544 [Abstract] [Full Text]  
  • Zhou, S., Hayward, S. D. (2001). Nuclear Localization of CBF1 Is Regulated by Interactions with the SMRT Corepressor Complex. Mol. Cell. Biol. 21: 6222-6232 [Abstract] [Full Text]  
  • Ronchini, C., Capobianco, A. J. (2001). Induction of Cyclin D1 Transcription and CDK2 Activity by Notchic: Implication for Cell Cycle Disruption in Transformation by Notchic. Mol. Cell. Biol. 21: 5925-5934 [Abstract] [Full Text]  
  • Iso, T., Sartorelli, V., Chung, G., Shichinohe, T., Kedes, L., Hamamori, Y. (2001). HERP, a New Primary Target of Notch Regulated by Ligand Binding. Mol. Cell. Biol. 21: 6071-6079 [Abstract] [Full Text]  
  • Prathapam, T., Kuhne, C., Hayman, M., Banks, L. (2001). Ski interacts with the evolutionarily conserved SNW domain of Skip. Nucleic Acids Res 29: 3469-3476 [Abstract] [Full Text]  
  • Lamar, E., Deblandre, G., Wettstein, D., Gawantka, V., Pollet, N., Niehrs, C., Kintner, C. (2001). Nrarp is a novel intracellular component of the Notch signaling pathway. Genes Dev. 15: 1885-1899 [Abstract] [Full Text]  
  • Allman, D., Karnell, F. G., Punt, J. A., Bakkour, S., Xu, L., Myung, P., Koretzky, G. A., Pui, J. C., Aster, J. C., Pear, W. S. (2001). Separation of Notch1 Promoted Lineage Commitment and Expansion/Transformation in Developing T Cells. JEM 194: 99-106 [Abstract] [Full Text]  
  • Wang, J., Shelly, L., Miele, L., Boykins, R., Norcross, M. A., Guan, E. (2001). Human Notch-1 Inhibits NF-{{kappa}}B Activity in the Nucleus Through a Direct Interaction Involving a Novel Domain. J. Immunol. 167: 289-295 [Abstract] [Full Text]  
  • Staal, F. J.T., Weerkamp, F., Langerak, A. W., Hendriks, R. W., Clevers, H. C. (2001). Transcriptional Control of T Lymphocyte Differentiation. Stem Cells 19: 165-179 [Abstract] [Full Text]  
  • Zhang, J., Chen, H., Weinmaster, G., Hayward, S. D. (2001). Epstein-Barr Virus BamHI-A Rightward Transcript-Encoded RPMS Protein Interacts with the CBF1-Associated Corepressor CIR To Negatively Regulate the Activity of EBNA2 and NotchIC. J. Virol. 75: 2946-2956 [Abstract] [Full Text]  
  • Aster, J. C., Xu, L., Karnell, F. G., Patriub, V., Pui, J. C., Pear, W. S. (2000). Essential Roles for Ankyrin Repeat and Transactivation Domains in Induction of T-Cell Leukemia by Notch1. Mol. Cell. Biol. 20: 7505-7515 [Abstract] [Full Text]  
  • Nicholas, J (2000). Evolutionary aspects of oncogenic herpesviruses. Mol. Pathol. 53: 222-237 [Abstract] [Full Text]  
  • Doyle, T. G., Wen, C., Greenwald, I. (2000). SEL-8, a nuclear protein required for LIN-12 and GLP-1 signaling in Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 97: 7877-7881 [Abstract] [Full Text]  
  • Leong, G. M., Subramaniam, N., Figueroa, J., Flanagan, J. L., Hayman, M. J., Eisman, J. A., Kouzmenko, A. P. (2001). Ski-interacting Protein Interacts with Smad Proteins to Augment Transforming Growth Factor-beta -dependent Transcription. J. Biol. Chem. 276: 18243-18248 [Abstract] [Full Text]  
  • Yamamoto, N., Yamamoto, S.-i., Inagaki, F., Kawaichi, M., Fukamizu, A., Kishi, N., Matsuno, K., Nakamura, K., Weinmaster, G., Okano, H., Nakafuku, M. (2001). Role of Deltex-1 as a Transcriptional Regulator Downstream of the Notch Receptor. J. Biol. Chem. 276: 45031-45040 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»