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Molecular and Cellular Biology, April 2000, p. 2660-2669, Vol. 20, No. 8
Sir William Dunn School of Pathology,
University of Oxford, Oxford OX1 3RE, United Kingdom
Received 1 October 1999/Returned for modification 10 November
1999/Accepted 24 January 2000
We have investigated how the upstream sequence element (USE) of the
lamin B2 poly(A) signal mediates efficient 3'-end formation. In vitro
analysis demonstrates that this USE increases both the efficiency of
3'-end cleavage and the processivity of poly(A) addition. Cross-linking
using selectively labeled synthetic RNAs confirms that cleavage
stimulation factor interacts with the sequences downstream of the
cleavage site, while electrophoresis mobility shift assays demonstrate
that the USE directly stabilizes the binding of the cleavage and
polyadenylation specificity factor to the poly(A) signal. Thus in
common with other poly(A) signals, the lamin B2 USE directly enhances
the binding of basal poly(A) factors. In addition, a novel 55-kDa
protein binds to the USE and the core poly(A) signal and appears to
inhibit cleavage. The binding activity of this factor appears to change
during the cell cycle, being greatest in S phase, when the lamin B2
gene is transcribed.
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Copyright © 2000, American Society for Microbiology. All rights reserved.
Recruitment of a Basal Polyadenylation Factor by the Upstream
Sequence Element of the Human Lamin B2 Polyadenylation Signal
and
*
Corresponding author. Mailing address: Sir William Dunn
School of Pathology, University of Oxford, South Parks Rd., Oxford OX1
3RE, United Kingdom. Phone: 01865 275566. Fax: 01865 275556. E-mail:
nicholas.proudfoot{at}path.ox.ac.uk.
Present address: Human Immunology Unit, Institute of Molecular
Medicine, University of Oxford, Headington, Oxford OX3 9DS, United Kingdom.
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