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Molecular and Cellular Biology, May 2000, p. 3187-3197, Vol. 20, No. 9
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Structure of a Polycomb Response Element and In Vitro Binding of Polycomb Group Complexes Containing GAGA Factor

Béatrice Horard, Christophe Tatout, Sylvain Poux, and Vincenzo Pirrotta^*

Department of Zoology, University of Geneva, CH1211 Geneva, Switzerland

Received 13 September 1999/Returned for modification 5 November 1999/Accepted 2 February 2000

Polycomb response elements (PREs) are regulatory sites that mediate the silencing of homeotic and other genes. The bxd PRE region from the Drosophila Ultrabithorax gene can be subdivided into subfragments of 100 to 200 bp that retain different degrees of PRE activity in vivo. In vitro, embryonic nuclear extracts form complexes containing Polycomb group (PcG) proteins with these fragments. PcG binding to some fragments is dependent on consensus sequences for the GAGA factor. Other fragments lack GAGA binding sites but can still bind PcG complexes in vitro. We show that the GAGA factor is a component of at least some types of PcG complexes and may participate in the assembly of PcG complexes at PREs.

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^* Corresponding author. Mailing address: Department of Zoology, University of Geneva, 30 quai Ernest Ansermet, CH1211 Geneva, Switzerland. Phone: (41 22) 702 6786. Fax: (41 22) 702 6776. E-mail: pirrotta{at}zoo.unige.ch.

Present address: Laboratoire BIOMOVE, UMR 6547, Université de Clermont Ferrand II, 63177 Aubiere Cedex, France.

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Molecular and Cellular Biology, May 2000, p. 3187-3197, Vol. 20, No. 9
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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