This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, S.-L.
Right arrow Articles by Prakash, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, S.-L.
Right arrow Articles by Prakash, L.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 2001, p. 185-188, Vol. 21, No. 1
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.1.185-188.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Requirement of DNA Polymerase eta  for Error-Free Bypass of UV-Induced CC and TC Photoproducts

Sung-Lim Yu, Robert E. Johnson, Satya Prakash, and Louise Prakash*

Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555-1061

Received 29 August 2000/Returned for modification 28 September 2000/Accepted 12 October 2000

The yeast RAD30-encoded DNA polymerase eta  (Poleta ) bypasses a cis-syn thymine-thymine dimer efficiently and accurately. Human DNA polymerase eta  functions similarly in the bypass of this lesion, and mutations in human Poleta result in the cancer prone syndrome, the variant form of xeroderma pigmentosum. UV light, however, also elicits the formation of cis-syn cyclobutane dimers and (6-4) photoproducts at 5'-CC-3' and 5'-TC-3' sites, and in both yeast and human DNA, UV-induced mutations occur primarily by 3' C to T transitions. Genetic studies presented here reveal a role for yeast Poleta in the error-free bypass of cyclobutane dimers and (6-4) photoproducts formed at CC and TC sites. Thus, by preventing UV mutagenesis at a wide spectrum of dipyrimidine sites, Poleta plays a pivotal role in minimizing the incidence of sunlight-induced skin cancers in humans.

* Corresponding author. Mailing address: University of Texas Medical Branch, Sealy Center for Molecular Science, 6.104 Medical Research Building, 11th and Mechanic Streets, Galveston, TX 77555-1061. Phone: (409) 747-8601. Fax: (409) 747-8608. E-mail: lprakash{at}scms.utmb.edu.

Molecular and Cellular Biology, January 2001, p. 185-188, Vol. 21, No. 1
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.1.185-188.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pages, V., Santa Maria, S. R., Prakash, L., Prakash, S. (2009). Role of DNA damage-induced replication checkpoint in promoting lesion bypass by translesion synthesis in yeast. Genes Dev. 23: 1438-1449 [Abstract] [Full Text]  
  • McCulloch, S. D., Kokoska, R. J., Garg, P., Burgers, P. M., Kunkel, T. A. (2009). The efficiency and fidelity of 8-oxo-guanine bypass by DNA polymerases {delta} and {eta}. Nucleic Acids Res 37: 2830-2840 [Abstract] [Full Text]  
  • Pages, V., Bresson, A., Acharya, N., Prakash, S., Fuchs, R. P., Prakash, L. (2008). Requirement of Rad5 for DNA Polymerase {zeta}-Dependent Translesion Synthesis in Saccharomyces cerevisiae. Genetics 180: 73-82 [Abstract] [Full Text]  
  • Skinner, A. M., Dan, C., Turker, M. S. (2008). The frequency of CC to TT tandem mutations in mismatch repair-deficient cells is increased in a cytosine run. Mutagenesis 23: 87-91 [Abstract] [Full Text]  
  • Acharya, N., Haracska, L., Prakash, S., Prakash, L. (2007). Complex Formation of Yeast Rev1 with DNA Polymerase {eta}. Mol. Cell. Biol. 27: 8401-8408 [Abstract] [Full Text]  
  • Gangavarapu, V., Prakash, S., Prakash, L. (2007). Requirement of RAD52 Group Genes for Postreplication Repair of UV-Damaged DNA in Saccharomyces cerevisiae. Mol. Cell. Biol. 27: 7758-7764 [Abstract] [Full Text]  
  • Johnson, R. E., Yu, S.-L., Prakash, S., Prakash, L. (2007). A Role for Yeast and Human Translesion Synthesis DNA Polymerases in Promoting Replication through 3-Methyl Adenine. Mol. Cell. Biol. 27: 7198-7205 [Abstract] [Full Text]  
  • Acharya, N., Johnson, R. E., Prakash, S., Prakash, L. (2006). Complex Formation with Rev1 Enhances the Proficiency of Saccharomyces cerevisiae DNA Polymerase {zeta} for Mismatch Extension and for Extension Opposite from DNA Lesions. Mol. Cell. Biol. 26: 9555-9563 [Abstract] [Full Text]  
  • Unk, I., Hajdu, I., Fatyol, K., Szakal, B., Blastyak, A., Bermudez, V., Hurwitz, J., Prakash, L., Prakash, S., Haracska, L. (2006). Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen. Proc. Natl. Acad. Sci. USA 103: 18107-18112 [Abstract] [Full Text]  
  • Gangavarapu, V., Haracska, L., Unk, I., Johnson, R. E., Prakash, S., Prakash, L. (2006). Mms2-Ubc13-Dependent and -Independent Roles of Rad5 Ubiquitin Ligase in Postreplication Repair and Translesion DNA Synthesis in Saccharomyces cerevisiae. Mol. Cell. Biol. 26: 7783-7790 [Abstract] [Full Text]  
  • Haracska, L., Unk, I., Prakash, L., Prakash, S. (2006). Ubiquitylation of yeast proliferating cell nuclear antigen and its implications for translesion DNA synthesis. Proc. Natl. Acad. Sci. USA 103: 6477-6482 [Abstract] [Full Text]  
  • Abdulovic, A. L., Jinks-Robertson, S. (2006). The in Vivo Characterization of Translesion Synthesis Across UV-Induced Lesions in Saccharomyces cerevisiae: Insights Into Pol{zeta}- and Pol{eta}-Dependent Frameshift Mutagenesis. Genetics 172: 1487-1498 [Abstract] [Full Text]  
  • Kennedy, R. D., D'Andrea, A. D. (2005). The Fanconi Anemia/BRCA pathway: new faces in the crowd. Genes Dev. 19: 2925-2940 [Abstract] [Full Text]  
  • Acharya, N., Haracska, L., Johnson, R. E., Unk, I., Prakash, S., Prakash, L. (2005). Complex Formation of Yeast Rev1 and Rev7 Proteins: a Novel Role for the Polymerase-Associated Domain. Mol. Cell. Biol. 25: 9734-9740 [Abstract] [Full Text]  
  • Johnson, R. E., Prakash, L., Prakash, S. (2005). Distinct mechanisms of cis-syn thymine dimer bypass by Dpo4 and DNA polymerase {eta}. Proc. Natl. Acad. Sci. USA 102: 12359-12364 [Abstract] [Full Text]  
  • Jaloszynski, P., Ohashi, E., Ohmori, H., Nishimura, S. (2005). Error-prone and inefficient replication across 8-hydroxyguanine (8-oxoguanine) in human and mouse ras gene fragments by DNA polymerase {kappa}. GENES CELLS 10: 543-550 [Abstract] [Full Text]  
  • Johansson, F., Lagerqvist, A., Erixon, K., Jenssen, D. (2004). A method to monitor replication fork progression in mammalian cells: nucleotide excision repair enhances and homologous recombination delays elongation along damaged DNA. Nucleic Acids Res 32: e157-e157 [Abstract] [Full Text]  
  • Washington, M. T., Minko, I. G., Johnson, R. E., Wolfle, W. T., Harris, T. M., Lloyd, R. S., Prakash, S., Prakash, L. (2004). Efficient and Error-Free Replication Past a Minor-Groove DNA Adduct by the Sequential Action of Human DNA Polymerases {iota} and {kappa}. Mol. Cell. Biol. 24: 5687-5693 [Abstract] [Full Text]  
  • Haracska, L., Torres-Ramos, C. A., Johnson, R. E., Prakash, S., Prakash, L. (2004). Opposing Effects of Ubiquitin Conjugation and SUMO Modification of PCNA on Replicational Bypass of DNA Lesions in Saccharomyces cerevisiae. Mol. Cell. Biol. 24: 4267-4274 [Abstract] [Full Text]  
  • Takasawa, K., Masutani, C., Hanaoka, F., Iwai, S. (2004). Chemical synthesis and translesion replication of a cis-syn cyclobutane thymine-uracil dimer. Nucleic Acids Res 32: 1738-1745 [Abstract] [Full Text]  
  • Washington, M. T., Johnson, R. E., Prakash, L., Prakash, S. (2003). The Mechanism of Nucleotide Incorporation by Human DNA Polymerase {eta} Differs from That of the Yeast Enzyme. Mol. Cell. Biol. 23: 8316-8322 [Abstract] [Full Text]  
  • Haracska, L., Prakash, L., Prakash, S. (2003). A mechanism for the exclusion of low-fidelity human Y-family DNA polymerases from base excision repair. Genes Dev. 17: 2777-2785 [Abstract] [Full Text]  
  • Jaloszynski, P., Masutani, C., Hanaoka, F., Perez, A. B., Nishimura, S. (2003). 8-Hydroxyguanine in a mutational hotspot of the c-Ha-ras gene causes misreplication, 'action-at-a-distance' mutagenesis and inhibition of replication. Nucleic Acids Res 31: 6085-6095 [Abstract] [Full Text]  
  • Washington, M. T., Prakash, L., Prakash, S. (2003). Mechanism of nucleotide incorporation opposite a thymine-thymine dimer by yeast DNA polymerase {eta}. Proc. Natl. Acad. Sci. USA 100: 12093-12098 [Abstract] [Full Text]  
  • Wolfle, W. T., Washington, M. T., Prakash, L., Prakash, S. (2003). Human DNA polymerase {kappa} uses template-primer misalignment as a novel means for extending mispaired termini and for generating single-base deletions. Genes Dev. 17: 2191-2199 [Abstract] [Full Text]  
  • Kozmin, S. G., Pavlov, Y. I., Kunkel, T. A., Sage, E. (2003). Roles of Saccharomyces cerevisiae DNA polymerases Pol{eta} and Pol{zeta} in response to irradiation by simulated sunlight. Nucleic Acids Res 31: 4541-4552 [Abstract] [Full Text]  
  • Washington, M. T., Helquist, S. A., Kool, E. T., Prakash, L., Prakash, S. (2003). Requirement of Watson-Crick Hydrogen Bonding for DNA Synthesis by Yeast DNA Polymerase {eta}. Mol. Cell. Biol. 23: 5107-5112 [Abstract] [Full Text]  
  • Stary, A., Kannouche, P., Lehmann, A. R., Sarasin, A. (2003). Role of DNA Polymerase {eta} in the UV Mutation Spectrum in Human Cells. J. Biol. Chem. 278: 18767-18775 [Abstract] [Full Text]  
  • Washington, M. T., Wolfle, W. T., Spratt, T. E., Prakash, L., Prakash, S. (2003). Yeast DNA polymerase eta makes functional contacts with the DNA minor groove only at the incoming nucleoside triphosphate. Proc. Natl. Acad. Sci. USA 100: 5113-5118 [Abstract] [Full Text]  
  • Lee, D.-H., Pfeifer, G. P. (2003). Deamination of 5-Methylcytosines within Cyclobutane Pyrimidine Dimers Is an Important Component of UVB Mutagenesis. J. Biol. Chem. 278: 10314-10321 [Abstract] [Full Text]  
  • Haracska, L., Prakash, S., Prakash, L. (2003). Yeast DNA Polymerase {zeta} Is an Efficient Extender of Primer Ends Opposite from 7,8-Dihydro-8-Oxoguanine and O6-Methylguanine. Mol. Cell. Biol. 23: 1453-1459 [Abstract] [Full Text]  
  • Johnson, R. E., Yu, S.-L., Prakash, S., Prakash, L. (2003). Yeast DNA polymerase zeta (zeta ) is essential for error-free replication past thymine glycol. Genes Dev. 17: 77-87 [Abstract] [Full Text]  
  • Haracska, L., Prakash, L., Prakash, S. (2002). Role of human DNA polymerase kappa as an extender in translesion synthesis. Proc. Natl. Acad. Sci. USA 99: 16000-16005 [Abstract] [Full Text]  
  • Prakash, S., Prakash, L. (2002). Translesion DNA synthesis in eukaryotes: A one- or two-polymerase affair. Genes Dev. 16: 1872-1883 [Full Text]  
  • Haracska, L., Prakash, S., Prakash, L. (2002). Yeast Rev1 Protein Is a G Template-specific DNA Polymerase. J. Biol. Chem. 277: 15546-15551 [Abstract] [Full Text]  
  • Torres-Ramos, C. A., Prakash, S., Prakash, L. (2002). Requirement of RAD5 and MMS2 for Postreplication Repair of UV-Damaged DNA in Saccharomyces cerevisiae. Mol. Cell. Biol. 22: 2419-2426 [Abstract] [Full Text]  
  • Zhang, H., Siede, W. (2002). UV-induced T->C transition at a TT photoproduct site is dependent on Saccharomyces cerevisiae polymerase {eta}in vivo. Nucleic Acids Res 30: 1262-1267 [Abstract] [Full Text]  
  • Haracska, L., Unk, I., Johnson, R. E., Phillips, B. B., Hurwitz, J., Prakash, L., Prakash, S. (2002). Stimulation of DNA Synthesis Activity of Human DNA Polymerase {kappa} by PCNA. Mol. Cell. Biol. 22: 784-791 [Abstract] [Full Text]  
  • Haracska, L., Johnson, R. E., Unk, I., Phillips, B. B., Hurwitz, J., Prakash, L., Prakash, S. (2001). Targeting of human DNA polymerase iota to the replication machinery via interaction with PCNA. Proc. Natl. Acad. Sci. USA 10.1073/pnas.261560798v1 [Abstract] [Full Text]  
  • Madril, A. C., Johnson, R. E., Washington, M. T., Prakash, L., Prakash, S. (2001). Fidelity and Damage Bypass Ability of Schizosaccharomyces pombe Eso1 Protein, Comprised of DNA Polymerase eta and Sister Chromatid Cohesion Protein Ctf7. J. Biol. Chem. 276: 42857-42862 [Abstract] [Full Text]  
  • Haracska, L., Johnson, R. E., Unk, I., Phillips, B., Hurwitz, J., Prakash, L., Prakash, S. (2001). Physical and Functional Interactions of Human DNA Polymerase {eta} with PCNA. Mol. Cell. Biol. 21: 7199-7206 [Abstract] [Full Text]  
  • Washington, M. T., Johnson, R. E., Prakash, L., Prakash, S. (2001). Accuracy of lesion bypass by yeast and human DNA polymerase eta. Proc. Natl. Acad. Sci. USA 98: 8355-8360 [Abstract] [Full Text]  
  • Johnson, R. E., Haracska, L., Prakash, S., Prakash, L. (2001). Role of DNA Polymerase {eta} in the Bypass of a (6-4) TT Photoproduct. Mol. Cell. Biol. 21: 3558-3563 [Abstract] [Full Text]  
  • Haracska, L., Unk, I., Johnson, R. E., Johansson, E., Burgers, P. M.J., Prakash, S., Prakash, L. (2001). Roles of yeast DNA polymerases {delta} and {zeta} and of Rev1 in the bypass of abasic sites. Genes Dev. 15: 945-954 [Abstract] [Full Text]  
  • You, Y.-H., Lee, D.-H., Yoon, J.-H., Nakajima, S., Yasui, A., Pfeifer, G. P. (2001). Cyclobutane Pyrimidine Dimers Are Responsible for the Vast Majority of Mutations Induced by UVB Irradiation in Mammalian Cells. J. Biol. Chem. 276: 44688-44694 [Abstract] [Full Text]  
  • Washington, M. T., Johnson, R. E., Prakash, L., Prakash, S. (2002). Human DINB1-encoded DNA polymerase kappa is a promiscuous extender of mispaired primer termini. Proc. Natl. Acad. Sci. USA 99: 1910-1914 [Abstract] [Full Text]  
  • Haracska, L., Johnson, R. E., Unk, I., Phillips, B. B., Hurwitz, J., Prakash, L., Prakash, S. (2001). Targeting of human DNA polymerase iota to the replication machinery via interaction with PCNA. Proc. Natl. Acad. Sci. USA 98: 14256-14261 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»