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Molecular and Cellular Biology, January 2001, p. 235-248, Vol. 21, No. 1
Institute for Microbiology and Genetics,
Georg-August University, D-37077 Göttingen, Germany
Received 21 August 2000/Returned for modification 22 September
2000/Accepted 3 October 2000
In budding yeast, the Rho-type GTPase Cdc42p is essential for cell
division and regulates pseudohyphal development and invasive growth.
Here, we isolated novel Cdc42p mutant proteins with
single-amino-acid substitutions that are sufficient to uncouple
functions of Cdc42p essential for cell division from regulatory
functions required for pseudohyphal development and invasive growth. In
haploid cells, Cdc42p is able to regulate invasive growth dependent on
and independent of FLO11 gene expression. In diploid cells,
Cdc42p regulates pseudohyphal development by controlling pseudohyphal
cell (PH cell) morphogenesis and invasive growth. Several of the Cdc42p
mutants isolated here block PH cell morphogenesis in response to
nitrogen starvation without affecting morphology or polarity of yeast
form cells in nutrient-rich conditions, indicating that these proteins
are impaired for certain signaling functions. Interaction studies
between development-specific Cdc42p mutants and known effector proteins
indicate that in addition to the p21-activated (PAK)-like protein
kinase Ste20p, the Cdc42p/Rac-interactive-binding domain containing
Gic1p and Gic2p proteins and the PAK-like protein kinase Skm1p might be
further effectors of Cdc42p that regulate pseudohyphal and invasive growth.
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.1.235-248.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Different Domains of the Essential GTPase Cdc42p
Required for Growth and Development of Saccharomyces
cerevisiae
*
Corresponding author. Mailing address: Institute for
Microbiology and Genetics, Georg-August University, Grisebachsr. 8, D-37077 Göttingen, Germany. Phone: (49) 551 39 38 17. Fax: (49)
551 39 38 20. E-mail: hmoesch{at}gwdg.de.
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