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Molecular and Cellular Biology, January 2001, p. 235-248, Vol. 21, No. 1
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.1.235-248.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Different Domains of the Essential GTPase Cdc42p Required for Growth and Development of Saccharomyces cerevisiae

Hans-Ulrich Mösch,* Tim Köhler, and Gerhard H. Braus

Institute for Microbiology and Genetics, Georg-August University, D-37077 Göttingen, Germany

Received 21 August 2000/Returned for modification 22 September 2000/Accepted 3 October 2000

In budding yeast, the Rho-type GTPase Cdc42p is essential for cell division and regulates pseudohyphal development and invasive growth. Here, we isolated novel Cdc42p mutant proteins with single-amino-acid substitutions that are sufficient to uncouple functions of Cdc42p essential for cell division from regulatory functions required for pseudohyphal development and invasive growth. In haploid cells, Cdc42p is able to regulate invasive growth dependent on and independent of FLO11 gene expression. In diploid cells, Cdc42p regulates pseudohyphal development by controlling pseudohyphal cell (PH cell) morphogenesis and invasive growth. Several of the Cdc42p mutants isolated here block PH cell morphogenesis in response to nitrogen starvation without affecting morphology or polarity of yeast form cells in nutrient-rich conditions, indicating that these proteins are impaired for certain signaling functions. Interaction studies between development-specific Cdc42p mutants and known effector proteins indicate that in addition to the p21-activated (PAK)-like protein kinase Ste20p, the Cdc42p/Rac-interactive-binding domain containing Gic1p and Gic2p proteins and the PAK-like protein kinase Skm1p might be further effectors of Cdc42p that regulate pseudohyphal and invasive growth.

* Corresponding author. Mailing address: Institute for Microbiology and Genetics, Georg-August University, Grisebachsr. 8, D-37077 Göttingen, Germany. Phone: (49) 551 39 38 17. Fax: (49) 551 39 38 20. E-mail: hmoesch{at}gwdg.de.

Molecular and Cellular Biology, January 2001, p. 235-248, Vol. 21, No. 1
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.1.235-248.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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