Nuclear Export of 60S Ribosomal Subunits Depends on Xpo1p and Requires a Nuclear Export Sequence-Containing Factor, Nmd3p, That Associates with the Large Subunit Protein Rpl10p

doi:10.1128/MCB.21.10.3405-3415.2001

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Molecular and Cellular Biology, May 2001, p. 3405-3415, Vol. 21, No. 10
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.10.3405-3415.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Nuclear Export of 60S Ribosomal Subunits Depends on Xpo1p and Requires a Nuclear Export Sequence-Containing Factor, Nmd3p, That Associates with the Large Subunit Protein Rpl10p

Olivier Gadal,¹ Daniela Strauß,¹ Jacques Kessl,² Bernard Trumpower,² David Tollervey,³ and Ed Hurt¹^,^*

Biochemie-Zentrum Heidelberg, D-69120 Heidelberg, Germany¹; Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755²; and Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom³

Received 7 December 2000/Returned for modification 17 January 2001/Accepted 19 February 2001

Nuclear export of ribosomes requires a subset of nucleoporins and the Ran system, but specific transport factors have notbeen identified. Using a large subunit reporter (Rpl25p-eGFP),we have isolated several temperature-sensitive ribosomal export(rix) mutants. One of these corresponds to the ribosomal proteinRpl10p, which interacts directly with Nmd3p, a conserved and essentialprotein associated with 60S subunits. We find that thermosensitivenmd3 mutants are impaired in large subunit export. Strikingly,Nmd3p shuttles between the nucleus and cytoplasm and is exportedby the nuclear export receptor Xpo1p. Moreover, we show that exportof 60S subunits is Xpo1p dependent. We conclude that nuclear exportof 60S subunits requires the nuclear export sequence-containingnonribosomal protein Nmd3p, which directly binds to the largesubunit proteinRpl10p.

^* Corresponding author. Mailing address: Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.Phone: 49-(0)6221-54-4173. Fax: 49-(0)6221-54-4369. E-mail: cg5{at}ix.urz.uni-heidelberg.de.

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