This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hay, D. C.
Right arrow Articles by Hay, R. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hay, D. C.
Right arrow Articles by Hay, R. T.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, May 2001, p. 3482-3490, Vol. 21, No. 10
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.10.3482-3490.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Interaction between hnRNPA1 and Ikappa Balpha Is Required for Maximal Activation of NF-kappa B-Dependent Transcription

David C. Hay,1 Graham D. Kemp,1 Catherine Dargemont,2 and Ronald T. Hay1,*

Institute of Biomolecular Sciences, School of Biology, University of St. Andrews, The North Haugh, St. Andrews, KY16 9ST, Scotland,1 and Institut Jacques Monod, UMR 7592, 75251 Paris Cedex 05, France2

Received 30 October 2000/Returned for modification 13 November 2000/Accepted 20 February 2001

Transcriptional activation of NF-kappa B is mediated by signal-induced phosphorylation and degradation of its inhibitor, Ikappa Balpha . NF-kappa B activation induces a rapid resynthesis of Ikappa Balpha which is responsible for postinduction repression of transcription. Following resynthesis, Ikappa Balpha translocates to the nucleus, removes template bound NF-kappa B, and exports NF-kappa B to the cytoplasm in a transcriptionally inactive form. Here we demonstrate that Ikappa Balpha interacts directly with another nucleocytoplasmic shuttling protein, hnRNPA1, both in vivo and in vitro. This interaction requires one of the N-terminal RNA binding domains of hnRNPA1 and the C-terminal region of Ikappa Balpha . Cells lacking hnRNPA1 are defective in NF-kappa B-dependent transcriptional activation, but the defect in these cells is complemented by ectopic expression of hnRNPA1. hnRNPA1 expression in these cells increased the amount of Ikappa Balpha degradation, compared to that of the control cells, in response to activation by Epstein-Barr virus latent membrane protein 1. Thus in addition to regulating mRNA processing and transport, hnRNPA1 also contributes to the control of NF-kappa B-dependent transcription.

* Corresponding author. Mailing address: Institute of Biomolecular Sciences, School of Biology, University of St. Andrews, The North Haugh, St. Andrews, KY16 9ST, Scotland, United Kingdom. Phone: 44 1334 463396. Fax: 44 1334 462595. E-mail: rth{at}st-and.ac.uk.

Molecular and Cellular Biology, May 2001, p. 3482-3490, Vol. 21, No. 10
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.10.3482-3490.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Mao, X., Gluck, N., Li, D., Maine, G. N., Li, H., Zaidi, I. W., Repaka, A., Mayo, M. W., Burstein, E. (2009). GCN5 is a required cofactor for a ubiquitin ligase that targets NF-{kappa}B/RelA. Genes Dev. 23: 849-861 [Abstract] [Full Text]  
  • Bowick, G. C., Spratt, H. M., Hogg, A. E., Endsley, J. J., Wiktorowicz, J. E., Kurosky, A., Luxon, B. A., Gorenstein, D. G., Herzog, N. K. (2009). Analysis of the Differential Host Cell Nuclear Proteome Induced by Attenuated and Virulent Hemorrhagic Arenavirus Infection. J. Virol. 83: 687-700 [Abstract] [Full Text]  
  • Jo, O. D., Martin, J., Bernath, A., Masri, J., Lichtenstein, A., Gera, J. (2008). Heterogeneous Nuclear Ribonucleoprotein A1 Regulates Cyclin D1 and c-myc Internal Ribosome Entry Site Function through Akt Signaling. J. Biol. Chem. 283: 23274-23287 [Abstract] [Full Text]  
  • Chateauvieux, S., Ichante, J.-L., Delorme, B., Frouin, V., Pietu, G., Langonne, A., Gallay, N., Sensebe, L., Martin, M. T., Moore, K. A., Charbord, P. (2007). Molecular profile of mouse stromal mesenchymal stem cells. Physiol. Genomics 29: 128-138 [Abstract] [Full Text]  
  • Beriault, V., Clement, J.-F., Levesque, K., LeBel, C., Yong, X., Chabot, B., Cohen, E. A., Cochrane, A. W., Rigby, W. F. C., Mouland, A. J. (2004). A Late Role for the Association of hnRNP A2 with the HIV-1 hnRNP A2 Response Elements in Genomic RNA, Gag, and Vpr Localization. J. Biol. Chem. 279: 44141-44153 [Abstract] [Full Text]  
  • Vassileva, M. T., Matunis, M. J. (2004). SUMO Modification of Heterogeneous Nuclear Ribonucleoproteins. Mol. Cell. Biol. 24: 3623-3632 [Abstract] [Full Text]  
  • Campillos, M., Lamas, J. R., Garcia, M. A., Bullido, M. J., Valdivieso, F., Vazquez, J. (2003). Specific interaction of heterogeneous nuclear ribonucleoprotein A1 with the -219T allelic form modulates APOE promoter activity. Nucleic Acids Res 31: 3063-3070 [Abstract] [Full Text]  
  • Nelson, G., Paraoan, L., Spiller, D. G., Wilde, G. J. C., Browne, M. A., Djali, P. K., Unitt, J. F., Sullivan, E., Floettmann, E., White, M. R. H. (2002). Multi-parameter analysis of the kinetics of NF-{kappa}B signalling and transcription in single living cells. J. Cell Sci. 115: 1137-1148 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»