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Molecular and Cellular Biology, May 2001, p. 3503-3513, Vol. 21, No. 10
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.10.3503-3513.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Human TAFII130 Is a Coactivator for NFATp

Loree J. Kim,dagger Anita G. Seto, Tuan N. Nguyen, and James A. Goodrich*

Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado 80309-0215

Received 6 October 2000/Returned for modification 3 December 2000/Accepted 20 February 2001

NFATp is one member of a family of transcriptional activators that regulate the expression of cytokine genes. To study mechanisms of NFATp transcriptional activation, we established a reconstituted transcription system consisting of human components that is responsive to activation by full-length NFATp. The TATA-associated factor (TAFII) subunits of the TFIID complex were required for NFATp-mediated activation in this transcription system, since TATA-binding protein (TBP) alone was insufficient in supporting activated transcription. In vitro interaction assays revealed that human TAFII130 (hTAFII130) and its Drosophila melanogaster homolog dTAFII110 bound specifically and reproducibly to immobilized NFATp. Sequences contained in the C-terminal domain of NFATp (amino acids 688 to 921) were necessary and sufficient for hTAFII130 binding. A partial TFIID complex assembled from recombinant hTBP, hTAFII250, and hTAFII130 supported NFATp-activated transcription, demonstrating the ability of hTAFII130 to serve as a coactivator for NFATp in vitro. Overexpression of hTAFII130 in Cos-1 cells inhibited NFATp activation of a luciferase reporter. These studies demonstrate that hTAFII130 is a coactivator for NFATp and represent the first biochemical characterization of the mechanism of transcriptional activation by the NFAT family of activators.

* Corresponding author. Mailing address: Department of Chemistry and Biochemistry, University of Colorado at Boulder, Campus Box 215, Boulder, CO 80309-0215. Phone: (303) 492-3273. Fax: (303) 492-5894. E-mail: james.goodrich{at}colorado.edu.

dagger Present address: University of Washington School of Law, Seattle, WA 98105-6617.

Molecular and Cellular Biology, May 2001, p. 3503-3513, Vol. 21, No. 10
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.10.3503-3513.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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