This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sutton, A.
Right arrow Articles by Sternglanz, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sutton, A.
Right arrow Articles by Sternglanz, R.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, May 2001, p. 3514-3522, Vol. 21, No. 10
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.10.3514-3522.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

A Novel Form of Transcriptional Silencing by Sum1-1 Requires Hst1 and the Origin Recognition Complex

Ann Sutton, Ryan C. Heller, Joseph Landry, Jennifer S. Choy, Agnieszka Sirko,dagger and Rolf Sternglanz*

Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794-5215

Received 20 November 2000/Returned for modification 3 January 2001/Accepted 15 February 2001

In the yeast Saccharomyces cerevisiae, a and alpha  mating-type information is stored in transcriptionally silenced cassettes called HML and HMR. Silencing of these loci, maintained by the formation of a specialized type of heterochromatin, requires trans-acting proteins and cis-acting elements. Proteins required for silencing include the Sir2 NAD+-dependent deacetylase, Sir3, and Sir4. Factors that bind to the cis elements at HMR and HML and that are important for silencing include the origin recognition complex (ORC). Mutations of any of these Sir proteins or combinations of cis elements result in loss of silencing. SUM1-1 was previously identified as a dominant mutation that restores silencing to HMR in the absence of either the Sir proteins or some of the cis elements. We have investigated the novel mechanism whereby Sum1-1 causes Sir-independent silencing at HMR and present the following findings: Sum1-1 requires the Sir2 homolog, Hst1, for silencing and most probably requires the NAD+-dependent deacetylase activity of this protein. Sum1-1 interacts strongly with ORC, and this strong interaction is dependent on HMR DNA. Furthermore, ORC is required for Sum1-1-mediated silencing at HMR. These observations lead to a model for Sum1-1 silencing of HMR in which Sum1-1 is recruited to HMR by binding to ORC. Sum1-1, in turn, recruits Hst1. Hst1 then deacetylates histones or other chromatin-associated proteins to cause chromatin condensation and transcriptional silencing.

* Corresponding author. Mailing address: Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215. Phone: (631) 632-8565. Fax: (631) 632-8575. E-mail: rolf{at}life.bio.sunysb.edu.

dagger Permanent address: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland.

Molecular and Cellular Biology, May 2001, p. 3514-3522, Vol. 21, No. 10
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.10.3514-3522.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Knott, S. R.V., Viggiani, C. J., Tavare, S., Aparicio, O. M. (2009). Genome-wide replication profiles indicate an expansive role for Rpd3L in regulating replication initiation timing or efficiency, and reveal genomic loci of Rpd3 function in Saccharomyces cerevisiae. Genes Dev. 23: 1077-1090 [Abstract] [Full Text]  
  • Safi, A., Wallace, K. A., Rusche, L. N. (2008). Evolution of New Function through a Single Amino Acid Change in the Yeast Repressor Sum1p. Mol. Cell. Biol. 28: 2567-2578 [Abstract] [Full Text]  
  • Darst, R. P., Garcia, S. N., Koch, M. R., Pillus, L. (2008). Slx5 Promotes Transcriptional Silencing and Is Required for Robust Growth in the Absence of Sir2. Mol. Cell. Biol. 28: 1361-1372 [Abstract] [Full Text]  
  • Mead, J., McCord, R., Youngster, L., Sharma, M., Gartenberg, M. R., Vershon, A. K. (2007). Swapping the Gene-Specific and Regional Silencing Specificities of the Hst1 and Sir2 Histone Deacetylases. Mol. Cell. Biol. 27: 2466-2475 [Abstract] [Full Text]  
  • Paterou, A., Walrad, P., Craddy, P., Fenn, K., Matthews, K. (2006). Identification and Stage-specific Association with the Translational Apparatus of TbZFP3, a CCCH Protein That Promotes Trypanosome Life-cycle Development. J. Biol. Chem. 281: 39002-39013 [Abstract] [Full Text]  
  • Yu, Q., Sandmeier, J., Xu, H., Zou, Y., Bi, X. (2006). Mechanism of the Long Range Anti-silencing Function of Targeted Histone Acetyltransferases in Yeast. J. Biol. Chem. 281: 3980-3988 [Abstract] [Full Text]  
  • Valenzuela, L., Gangadharan, S., Kamakaka, R. T. (2006). Analyses of SUM1-1-Mediated Long-Range Repression. Genetics 172: 99-112 [Abstract] [Full Text]  
  • Ueki, S., Citovsky, V. (2005). Identification of an interactor of cadmium ion-induced glycine-rich protein involved in regulation of callose levels in plant vasculature. Proc. Natl. Acad. Sci. USA 102: 12089-12094 [Abstract] [Full Text]  
  • Irlbacher, H., Franke, J., Manke, T., Vingron, M., Ehrenhofer-Murray, A. E. (2005). Control of replication initiation and heterochromatin formation in Saccharomyces cerevisiae by a regulator of meiotic gene expression. Genes Dev. 19: 1811-1822 [Abstract] [Full Text]  
  • Lynch, P. J., Fraser, H. B., Sevastopoulos, E., Rine, J., Rusche, L. N. (2005). Sum1p, the Origin Recognition Complex, and the Spreading of a Promoter-Specific Repressor in Saccharomyces cerevisiae. Mol. Cell. Biol. 25: 5920-5932 [Abstract] [Full Text]  
  • Geissenhoner, A., Weise, C., Ehrenhofer-Murray, A. E. (2004). Dependence of ORC Silencing Function on NatA-Mediated N{alpha} Acetylation in Saccharomyces cerevisiae. Mol. Cell. Biol. 24: 10300-10312 [Abstract] [Full Text]  
  • Wang, Y., Tzfira, T., Gaba, V., Citovsky, V., Palukaitis, P., Gal-On, A. (2004). Functional analysis of the Cucumber mosaic virus 2b protein: pathogenicity and nuclear localization. J. Gen. Virol. 85: 3135-3147 [Abstract] [Full Text]  
  • Buck, S. W., Gallo, C. M., Smith, J. S. (2004). Diversity in the Sir2 family of protein deacetylases. J. Leukoc. Biol. 75: 939-950 [Abstract] [Full Text]  
  • Hess, D., Liu, B., Roan, N. R., Sternglanz, R., Winston, F. (2004). Spt10-Dependent Transcriptional Activation in Saccharomyces cerevisiae Requires both the Spt10 Acetyltransferase Domain and Spt21. Mol. Cell. Biol. 24: 135-143 [Abstract] [Full Text]  
  • Andrulis, E. D., Zappulla, D. C., Alexieva-Botcheva, K., Evangelista, C., Sternglanz, R. (2004). One-Hybrid Screens at the Saccharomyces cerevisiae HMR Locus Identify Novel Transcriptional Silencing Factors. Genetics 166: 631-635 [Abstract] [Full Text]  
  • Hirao, M., Posakony, J., Nelson, M., Hruby, H., Jung, M., Simon, J. A., Bedalov, A. (2003). Identification of Selective Inhibitors of NAD+-dependent Deacetylases Using Phenotypic Screens in Yeast. J. Biol. Chem. 278: 52773-52782 [Abstract] [Full Text]  
  • Bedalov, A., Hirao, M., Posakony, J., Nelson, M., Simon, J. A. (2003). NAD+-Dependent Deacetylase Hst1p Controls Biosynthesis and Cellular NAD+ Levels in Saccharomyces cerevisiae. Mol. Cell. Biol. 23: 7044-7054 [Abstract] [Full Text]  
  • Pierce, M., Benjamin, K. R., Montano, S. P., Georgiadis, M. M., Winter, E., Vershon, A. K. (2003). Sum1 and Ndt80 Proteins Compete for Binding to Middle Sporulation Element Sequences That Control Meiotic Gene Expression. Mol. Cell. Biol. 23: 4814-4825 [Abstract] [Full Text]  
  • McCord, R., Pierce, M., Xie, J., Wonkatal, S., Mickel, C., Vershon, A. K. (2003). Rfm1, a Novel Tethering Factor Required To Recruit the Hst1 Histone Deacetylase for Repression of Middle Sporulation Genes. Mol. Cell. Biol. 23: 2009-2016 [Abstract] [Full Text]  
  • Pak, J., Segall, J. (2002). Regulation of the Premiddle and Middle Phases of Expression of the NDT80 Gene during Sporulation of Saccharomyces cerevisiae. Mol. Cell. Biol. 22: 6417-6429 [Abstract] [Full Text]  
  • Grunweller, A., Ehrenhofer-Murray, A. E. (2002). A Novel Yeast Silencer: The 2{micro} Origin of Saccharomyces cerevisiae Has HST3-, MIG1- and SIR-Dependent Silencing Activity. Genetics 162: 59-71 [Abstract] [Full Text]  
  • Tzfira, T., Vaidya, M., Citovsky, V. (2002). Increasing plant susceptibility to Agrobacterium infection by overexpression of the Arabidopsis nuclear protein VIP1. Proc. Natl. Acad. Sci. USA 99: 10435-10440 [Abstract] [Full Text]  
  • Rusche, L. N., Kirchmaier, A. L., Rine, J. (2002). Ordered Nucleation and Spreading of Silenced Chromatin in Saccharomyces cerevisiae. Mol. Biol. Cell 13: 2207-2222 [Abstract] [Full Text]  
  • Huang, Y. (2002). Transcriptional silencing in Saccharomyces cerevisiae and Schizosaccharomyces pombe. Nucleic Acids Res 30: 1465-1482 [Abstract] [Full Text]  
  • Bedalov, A., Gatbonton, T., Irvine, W. P., Gottschling, D. E., Simon, J. A. (2001). Identification of a small molecule inhibitor of Sir2p. Proc. Natl. Acad. Sci. USA 98: 15113-15118 [Abstract] [Full Text]  
  • Armstrong, C. M., Kaeberlein, M., Imai, S. I., Guarente, L. (2002). Mutations in Saccharomyces cerevisiae Gene SIR2 Can Have Differential Effects on In Vivo Silencing Phenotypes and In Vitro Histone Deacetylation Activity. Mol. Biol. Cell 13: 1427-1438 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»