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Molecular and Cellular Biology, June 2001, p. 3652-3661, Vol. 21, No. 11
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.11.3652-3661.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

p53 Stimulates TFIID-TFIIA-Promoter Complex Assembly, and p53-T Antigen Complex Inhibits TATA Binding Protein-TATA Interaction

Jun Xing, Hilary M. Sheppard,dagger Siska I. Corneillie, and Xuan Liu*

Department of Biochemistry, University of California, Riverside, California 92521

Received 19 January 2001/Returned for modification 26 February 2001/Accepted 7 March 2001

Simian virus 40 large T antigen has been shown to inhibit p53-mediated transcription once tethered to p53-responsive promoters through interaction with p53. In this study we report that p53 stimulates transcription by enhancing the recruitment of the basal transcription factors, TFIIA and TFIID, on the promoter (the DA complex) and by inducing a conformational change in the DA complex. Significantly, we have demonstrated that T antigen inhibits p53-mediated transcription by blocking this ability of p53. We investigated the mechanism for this inhibition and found that DA complex formation was resistant to T-antigen repression when the TFIID-DNA complex was formed prior to addition of p53-T antigen complex, indicating that the T antigen, once tethered to the promoter by p53, targets TFIID. Further, we have shown that the p53-T antigen complex prevents the TATA binding protein from binding to the TATA box. Thus, these data suggest a detailed mechanism by which p53 activates transcription and by which T antigen inhibits p53-mediated transcription.

* Corresponding author. Mailing address: Department of Biochemistry, University of California, Riverside, CA 92521. Phone: (909) 787-4350. Fax: (909) 787-4434. E-mail: xuan.liu{at}ucr.edu.

dagger Present address: Department of Biochemistry, University of Leicester, Leicester LE1 7RH, United Kingdom.

Molecular and Cellular Biology, June 2001, p. 3652-3661, Vol. 21, No. 11
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.11.3652-3661.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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