Identification of a Phospholipase C-{gamma}1 (PLC-{gamma}1) SH3 Domain-Binding Site in SLP-76 Required for T-Cell Receptor-Mediated Activation of PLC-{gamma}1 and NFAT

doi:10.1128/MCB.21.13.4208-4218.2001

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Molecular and Cellular Biology, July 2001, p. 4208-4218, Vol. 21, No. 13
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.13.4208-4218.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of a Phospholipase C- $gamma$ 1 (PLC- $gamma$ 1) SH3 Domain-Binding Site in SLP-76 Required for T-Cell Receptor-Mediated Activation of PLC- $gamma$ 1 and NFAT

Deborah Yablonski,¹ Theresa Kadlecek,² and Arthur Weiss²^,^*

Department of Pharmacology, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Bat Galim, Haifa 31096, Israel,¹ and Department of Medicine, Department of Microbiology and Immunology, and Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California 94143-0795²

Received 9 October 2000/Returned for modification 14 December 2000/Accepted 4 April 2001

SLP-76 is an adapter protein required for T-cell receptor (TCR) signaling. In particular, TCR-induced tyrosine phosphorylationand activation of phospholipase C- $gamma$ 1 (PLC- $gamma$ 1), and the resultantTCR-inducible gene expression, depend on SLP-76. Nonetheless,the mechanisms by which SLP-76 mediates PLC- $gamma$ 1 activation arenot well understood. We now demonstrate that SLP-76 directly interactswith the Src homology 3 (SH3) domain of PLC- $gamma$ 1. Structure-functionanalysis of SLP-76 revealed that each of the previously definedprotein-protein interaction domains can be individually deletedwithout completely disrupting SLP-76 function. Additional deletionmutations revealed a new, 67-amino-acid functional domain withinthe proline-rich region of SLP-76, which we have termed the P-1domain. The P-1 domain mediates a constitutive interaction ofSLP-76 with the SH3 domain of PLC- $gamma$ 1 and is required for TCR-mediatedactivation of Erk, PLC- $gamma$ 1, and NFAT (nuclear factor of activatedT cells). The adjacent Gads-binding domain of SLP-76, also withinthe proline-rich region, mediates inducible recruitment of SLP-76to a PLC- $gamma$ 1-containing complex via the recruitment of both PLC- $gamma$ 1and Gads to another cell-type-specific adapter, LAT. Thus, TCR-inducedactivation of PLC- $gamma$ 1 entails the binding of PLC- $gamma$ 1 to both LATand SLP-76, a finding that may underlie the requirement for bothLAT and SLP-76 to mediate the optimal activation of PLC- $gamma$ 1.

^* Corresponding author. Mailing address: Department of Medicine, Department of Microbiology and Immunology, and Howard HughesMedical Institute, U426, Box 0795, University of California, SanFrancisco, 533 Parnassus Ave., San Francisco, CA 94143-0795. Phone:(415) 476-1291. Fax: (415) 502-5081. E-mail: aweiss{at}medicine.ucsf.edu.

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Identification of a Phospholipase C-1 (PLC-1) SH3 Domain-Binding Site in SLP-76 Required for T-Cell Receptor-Mediated Activation of PLC-1 and NFAT

Identification of a Phospholipase C- $gamma$ 1 (PLC- $gamma$ 1) SH3 Domain-Binding Site in SLP-76 Required for T-Cell Receptor-Mediated Activation of PLC- $gamma$ 1 and NFAT