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Molecular and Cellular Biology, July 2001, p. 4219-4232, Vol. 21, No. 13
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.13.4219-4232.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The Yeast hnRNP-Like Proteins Yra1p and Yra2p Participate in mRNA Export through Interaction with Mex67p

Daniel Zenklusen, Patrizia Vinciguerra, Yvan Strahm, and Françoise Stutz*

Institute of Microbiology, Centre Hospitalier Universitaire Vaudois, 1012 Lausanne, Switzerland

Received 30 October 2000/Returned for modification 29 March 2001/Accepted 10 April 2001

Yra1p is an essential nuclear protein which belongs to the evolutionarily conserved REF (RNA and export factor binding proteins) family of hnRNP-like proteins. Yra1p contributes to mRNA export in vivo and directly interacts with RNA and the shuttling mRNP export receptor Mex67p in vitro. Here we describe a second nonessential Saccharomyces cerevisiae family member, called Yra2p, which is able to complement a YRA1 deletion when overexpressed. Like other REF proteins, Yra1p and Yra2p consist of two highly conserved N- and C-terminal boxes and a central RNP-like RNA-binding domain (RBD). These conserved regions are separated by two more variable regions, N-vr and C-vr. Surprisingly, the deletion of a single conserved box or the deletion of the RBD in Yra1p does not affect viability. Consistently, neither the conserved N and C boxes nor the RBD is required for Mex67p and RNA binding in vitro. Instead, the N-vr and C-vr regions both interact with Mex67p and RNA. We further show that Yra1 deletion mutants which poorly interact with Mex67p in vitro affect the association of Mex67p with mRNP complexes in vivo and are paralleled by poly(A)+ RNA export defects. These observations support the idea that Yra1p promotes mRNA export by facilitating the recruitment of Mex67p to the mRNP.

* Corresponding author. Mailing address: Institute of Microbiology, Centre Hospitalier Universitaire Vaudois, 44 Rue du Bugnon, 1011 Lausanne, Switzerland. Phone: 41 21 314 40 84. Fax: 41 21 314 40 95. E-mail: fstutz{at}hola.hospvd.ch.

Molecular and Cellular Biology, July 2001, p. 4219-4232, Vol. 21, No. 13
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.13.4219-4232.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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