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Molecular and Cellular Biology, July 2001, p. 4311-4320, Vol. 21, No. 13
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.13.4311-4320.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Essential Roles of Snf5p in Snf-Swi Chromatin Remodeling In Vivo

Fuqiang Geng, Yixue Cao,dagger and Brehon C. Laurent*

Department of Microbiology and Immunology and Morse Institute of Molecular Biology and Genetics, State University of New York, Brooklyn, New York 11203

Received 30 January 2001/Returned for modification 13 March 2001/Accepted 12 April 2001

Snf-Swi, the prototypical ATP-dependent nucleosome-remodeling complex, regulates transcription of a subset of yeast genes. With the exception of Snf2p, the ATPase subunit, the functions of the other components are unknown. We have investigated the role of the conserved Snf-Swi core subunit Snf5p through characterization of two conditional snf5 mutants. The mutants contain single amino acid alterations of invariant or conserved residues that abolish Snf-Swi-dependent transcription by distinct mechanisms. One mutation impairs Snf-Swi assembly and, consequently, its stable association with a target promoter. The other blocks a postrecruitment catalytic remodeling step. These findings suggest that Snf5p coordinates the assembly and nucleosome-remodeling activities of Snf-Swi.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Morse Institute of Moleular Biology and Genetics, S.U.N.Y., 450 Clarkson Ave., Box 44, Brooklyn, NY 11203-2098. Phone: (718) 270-3755. Fax: (718) 270-2656. E-mail: blaurent{at}netmail.hscbklyn.edu.

dagger Present address: Department of Pharmacology, University of California, San Diego, CA 92093.

Molecular and Cellular Biology, July 2001, p. 4311-4320, Vol. 21, No. 13
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.13.4311-4320.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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