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Molecular and Cellular Biology, July 2001, p. 4321-4329, Vol. 21, No. 13
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.13.4321-4329.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of a Peroxisomal ATP Carrier Required for Medium-Chain Fatty Acid beta -Oxidation and Normal Peroxisome Proliferation in Saccharomyces cerevisiae

Carlo W. T. van Roermund,1 Roy Drissen,1 Marlene van den Berg,2 Lodewijk Ijlst,1 Ewald H. Hettema,2 Henk F. Tabak,2 Hans R. Waterham,1,3 and Ronald J. A. Wanders1,3,*

University of Amsterdam, Academic Medical Centre, Departments of Clinical Chemistry,1 Biochemistry,2 and Paediatrics,3 Emma Children's Hospital, 1100 DE Amsterdam, The Netherlands

Received 18 December 2000/Returned for modification 6 February 2001/Accepted 4 April 2001

We have characterized the role of YPR128cp, the orthologue of human PMP34, in fatty acid metabolism and peroxisomal proliferation in Saccharomyces cerevisiae. YPR128cp belongs to the mitochondrial carrier family (MCF) of solute transporters and is localized in the peroxisomal membrane. Disruption of the YPR128c gene results in impaired growth of the yeast with the medium-chain fatty acid (MCFA) laurate as a single carbon source, whereas normal growth was observed with the long-chain fatty acid (LCFA) oleate. MCFA but not LCFA beta -oxidation activity was markedly reduced in intact ypr128cDelta mutant cells compared to intact wild-type cells, but comparable activities were found in the corresponding lysates. These results imply that a transport step specific for MCFA beta -oxidation is impaired in ypr128cDelta cells. Since MCFA beta -oxidation in peroxisomes requires both ATP and CoASH for activation of the MCFAs into their corresponding coenzyme A esters, we studied whether YPR128cp is an ATP carrier. For this purpose we have used firefly luciferase targeted to peroxisomes to measure ATP consumption inside peroxisomes. We show that peroxisomal luciferase activity was strongly reduced in intact ypr128cDelta mutant cells compared to wild-type cells but comparable in lysates of both cell strains. We conclude that YPR128cp most likely mediates the transport of ATP across the peroxisomal membrane.

* Corresponding author. Mailing address: University of Amsterdam, Academic Medical Centre, Meibergdreef 9 (Room F0-224), 1105 AZ Amsterdam, The Netherlands. Phone: 31-20-5662427. Fax: 31-20-6962596. E-mail: wanders{at}amc.uva.nl.

Molecular and Cellular Biology, July 2001, p. 4321-4329, Vol. 21, No. 13
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.13.4321-4329.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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