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Molecular and Cellular Biology, July 2001, p. 4413-4424, Vol. 21, No. 13
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.13.4413-4424.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Human Pex19p Binds Peroxisomal Integral Membrane
Proteins at Regions Distinct from Their Sorting Sequences
Marc
Fransen,*
Tine
Wylin,
Chantal
Brees,
Guy P.
Mannaerts, and
Paul P.
Van
Veldhoven*
Katholieke Universiteit Leuven, Campus
Gasthuisberg (O/N), Departement Moleculaire Celbiologie, Afdeling
Farmacologie, B-3000 Leuven, Belgium
Received 10 January 2001/Returned for modification 13 February
2001/Accepted 10 April 2001
The molecular machinery underlying peroxisomal membrane biogenesis
is not well understood. The observation that cells deficient in the
peroxins Pex3p, Pex16p, and Pex19p lack peroxisomal membrane structures
suggests that these molecules are involved in the initial stages of
peroxisomal membrane formation. Pex19p, a predominantly cytosolic
protein that can be farnesylated, binds multiple peroxisomal integral
membrane proteins, and it has been suggested that it functions
as a soluble receptor for the targeting of peroxisomal membrane
proteins (PMPs) to the peroxisome. An alternative view proposes that
Pex19p functions as a chaperone at the peroxisomal membrane. Here, we
show that the peroxisomal sorting determinants and the Pex19p-binding
domains of a number of PMPs are distinct entities. In addition, we
extend the list of peroxins with which human Pex19p interacts to
include the PMP Pex16p and show that Pex19p's CaaX prenylation motif
is an important determinant in the affinity of Pex19p for Pex10p,
Pex11p
, Pex12p, and Pex13p.
*
Corresponding author. Mailing address for Marc Fransen:
Katholieke Universiteit Leuven, Campus Gasthuisberg (O/N), Departement Moleculaire Celbiologie, Afdeling Farmacologie, Herestraat 49, B-3000
Leuven, Belgium. Phone: 32-16-345786. Fax: 32-16-345699. E-mail:
marc.fransen{at}med.kuleuven.ac.be. Mailing address
for Paul P. Van Veldhoven: Katholieke Universiteit Leuven, Campus
Gasthuisberg (O/N), Departement Moleculaire Celbiologie, Afdeling
Farmacologie, Herestraat 49, B-3000 Leuven, Belgium. Phone:
32-16-345802. Fax: 32-16-345699. E-mail:
paul.vanveldhoven{at}med.kuleuven.ac.be.
Molecular and Cellular Biology, July 2001, p. 4413-4424, Vol. 21, No. 13
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.13.4413-4424.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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