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Molecular and Cellular Biology, July 2001, p. 4441-4452, Vol. 21, No. 14
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.14.4441-4452.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Phosphorylation of MafA Is Essential for Its Transcriptional and Biological Properties

Sofia Benkhelifa, Sylvain Provot, Eugène Nabais, Alain Eychène, Georges Calothy, and Marie-Paule Felder-Schmittbuhl*

UMR 146 CNRS-Institut Curie, Centre Universitaire, 91405 Orsay cedex, France

Received 6 November 2000/Returned for modification 5 January 2001/Accepted 21 April 2001

We previously described the identification of quail MafA, a novel transcription factor of the Maf bZIP (basic region leucine zipper) family, expressed in the differentiating neuroretina (NR). In the present study, we provide the first evidence that MafA is phosphorylated and that its biological properties strongly rely upon phosphorylation of serines 14 and 65, two residues located in the transcriptional activating domain within a consensus for phosphorylation by mitogen-activated protein kinases and which are conserved among Maf proteins. These residues are phosphorylated by ERK2 but not by p38, JNK, and ERK5 in vitro. However, the contribution of the MEK/ERK pathway to MafA phosphorylation in vivo appears to be moderate, implicating another kinase. The integrity of serine 14 and serine 65 residues is required for transcriptional activity, since their mutation into alanine severely impairs MafA capacity to activate transcription. Furthermore, we show that the MafA S14A/S65A mutant displays reduced capacity to induce expression of QR1, an NR-specific target of Maf proteins. Likewise, the integrity of serines 14 and 65 is essential for the MafA ability to stimulate expression of crystallin genes in NR cells and to induce NR-to-lens transdifferentiation. Thus, the MafA capacity to induce differentiation programs is dependent on its phosphorylation.

* Corresponding author. Mailing address: UMR 146 CNRS-Institut Curie, Bâtiment 110, Centre Universitaire, 91405 Orsay cedex, France. Phone: 33 1 69 86 30 73. Fax: 33 1 69 07 45 25. E-mail: Marie-Paule.Felder{at}curie.u-psud.fr.

Molecular and Cellular Biology, July 2001, p. 4441-4452, Vol. 21, No. 14
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.14.4441-4452.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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