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Molecular and Cellular Biology, July 2001, p. 4614-4625, Vol. 21, No. 14
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.14.4614-4625.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Inhibition of Androgen Receptor-Mediated Transcription by Amino-Terminal Enhancer of split

Xin Yu,1 Peng Li,2 Robert G. Roeder,1 and Zhengxin Wang1,*

Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York 10021,1 and Department of Pathology, New York University Medical Center, New York, New York 100162

Received 13 February 2001/Returned for modification 21 March 2001/Accepted 18 April 2001

A yeast two-hybrid assay has identified an androgen-dependent interaction of androgen receptor (AR) with amino-terminal enhancer of split (AES), a member of the highly conserved Groucho/TLE family of corepressors. Full-length AR, as well as the N-terminal fragment of AR, showed direct interactions with AES in in vitro protein-protein interaction assays. AES specifically inhibited AR-mediated transcription in a well-defined cell-free transcription system and interacted specifically with the basal transcription factor (TFIIE) in HeLa nuclear extract. These observations implicate AES as a selective repressor of ligand-dependent AR-mediated transcription that acts by directly interacting with AR and by targeting the basal transcription machinery.

* Corresponding author. Mailing address: The Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, 1230 York Ave., New York, NY 10021. Phone: (212) 327-7604. Fax: (212) 327-7949. E-mail: wangz{at}rockvax.rockefeller.edu.

Molecular and Cellular Biology, July 2001, p. 4614-4625, Vol. 21, No. 14
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.14.4614-4625.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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