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Molecular and Cellular Biology, August 2001, p. 5031-5040, Vol. 21, No. 15
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.15.5031-5040.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Maf1p, a Negative Effector of RNA Polymerase III in
Saccharomyces cerevisiae
Krzysztof
Pluta,1
Olivier
Lefebvre,2
Nancy C.
Martin,3
Wieslaw J.
Smagowicz,1
David R.
Stanford,4
Steven R.
Ellis,3
Anita K.
Hopper,4
Andre
Sentenac,2 and
Magdalena
Boguta1,*
Department of Genetics, Institute of Biochemistry and
Biophysics, Polish Academy of Sciences, 02 106 Warsaw,
Poland1; Department of Biochemistry,
University of Louisville Medical Center, Louisville, Kentucky
402923; Department of Biochemistry
and Molecular Biology, Milton S. Hershey Medical Center, Pennsylvania
State University, Hershey, Pennsylvania
170334; and Service de Biochimie et de
Génétique Moléculaire, CEA/Saclay, F-91191
Gif-sur-Yvette Cedex, France2
Received 1 February 2001/Returned for modification 2 March
2001/Accepted 24 April 2001
Although yeast RNA polymerase III (Pol III) and the auxiliary
factors TFIIIC and TFIIIB are well characterized, the mechanisms of
class III gene regulation are poorly understood. Previous studies identified MAF1, a gene that affects tRNA suppressor
efficiency and interacts genetically with Pol III. We show here that
tRNA levels are elevated in maf1 mutant cells. In keeping
with the higher levels of tRNA observed in vivo, the in vitro rate of
Pol III RNA synthesis is significantly increased in maf1
cell extracts. Mutations in the RPC160 gene encoding the
largest subunit of Pol III which reduce tRNA levels were identified as
suppressors of the maf1 growth defect. Interestingly, Maf1p
is located in the nucleus and coimmunopurifies with epitope-tagged RNA
Pol III. These results indicate that Maf1p acts as a negative effector of Pol III synthesis. This potential regulator of Pol III transcription is likely conserved since orthologs of Maf1p are present in other eukaryotes, including humans.
*
Corresponding author. Mailing address: Department of
Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02 106 Warsaw, Poland. Phone: (48 22)659-70-72, ext. 1312. Fax: (48)39121623. E-mail:
magda{at}ibbrain.ibb.waw.pl.
Molecular and Cellular Biology, August 2001, p. 5031-5040, Vol. 21, No. 15
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.15.5031-5040.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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