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Molecular and Cellular Biology, August 2001, p. 5242-5255, Vol. 21, No. 15
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.15.5242-5255.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Drosophila Med6 Is Required for Elevated Expression of a Large but Distinct Set of Developmentally Regulated Genes

Byung Soo Gim,1,2,3 Jin Mo Park,1,dagger Jeong Ho Yoon,4 Changwon Kang,2 and Young-Joon Kim1,*

Department of Biochemistry, National Creative Research Initiative Center for Genome Regulation, Yonsei University,1 and Digital Genomics Inc.,4 Seoul 120-749, Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305-701,2 and Samsung Biomedical Research Institute, Seoul 135-230,3 Korea

Received 19 March 2001/Returned for modification 24 April 2001/Accepted 27 April 2001

Mediator is the evolutionarily conserved coactivator required for the integration and recruitment of diverse regulatory signals to basal transcription machinery. To elucidate the functions of metazoan Mediator, we isolated Drosophila melanogaster Med6 mutants. dMed6 is essential for viability and/or proliferation of most cells. dMed6 mutants failed to pupate and died in the third larval instar with severe proliferation defects in imaginal discs and other larval mitotic cells. cDNA microarray, quantitative reverse transcription-PCR, and in situ expression analyses of developmentally regulated genes in dMed6 mutants showed that transcriptional activation of many, but not all, genes was affected. Among the genes found to be affected were some that play a role in cell proliferation and metabolism. Therefore, dMed6 is required in most cells for transcriptional regulation of many genes important for diverse aspects of Drosophila development.

* Corresponding author. Mailing address: National Creative Research Initiative Center for Genome Regulation, Department of Biochemistry, Yonsei University, Shinchon-dong, Seodaemun-ku, Seoul 440-746, Korea. Phone: 82-2-2123-2628. Fax: 82-2-312-8834. E-mail: yjkim{at}yonsei.ac.kr.

dagger Present address: Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093-0636.

Molecular and Cellular Biology, August 2001, p. 5242-5255, Vol. 21, No. 15
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.15.5242-5255.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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