This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bose, A.
Right arrow Articles by Czech, M. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bose, A.
Right arrow Articles by Czech, M. P.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, August 2001, p. 5262-5275, Vol. 21, No. 15
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.15.5262-5275.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Galpha 11 Signaling through ARF6 Regulates F-Actin Mobilization and GLUT4 Glucose Transporter Translocation to the Plasma Membrane

Avirup Bose, Andrew D. Cherniack, Stephen E. Langille,dagger Sarah M. C. Nicoloro, Joanne M. Buxton, Jin G. Park, Anil Chawla, and Michael P. Czech*

Program in Molecular Medicine and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical Center, Worcester, Massachusetts 01605

Received 11 December 2000/Returned for modification 8 February 2001/Accepted 30 April 2001

The action of insulin to recruit the intracellular GLUT4 glucose transporter to the plasma membrane of 3T3-L1 adipocytes is mimicked by endothelin 1, which signals through trimeric Galpha q or Galpha 11 proteins. Here we report that murine Galpha 11 is most abundant in fat and that expression of the constitutively active form of Galpha 11 [Galpha 11(Q209L)] in 3T3-L1 adipocytes causes recruitment of GLUT4 to the plasma membrane and stimulation of 2-deoxyglucose uptake. In contrast to the action of insulin on GLUT4, the effects of endothelin 1 and Galpha 11 were not inhibited by the phosphatidylinositol 3-kinase inhibitor wortmannin at 100 nM. Signaling by insulin, endothelin 1, or Galpha 11(Q209L) also mobilized cortical F-actin in cultured adipocytes. Importantly, GLUT4 translocation caused by all three agents was blocked upon disassembly of F-actin by latrunculin B, suggesting that the F-actin polymerization caused by these agents may be required for their effects on GLUT4. Remarkably, expression of a dominant inhibitory form of the actin-regulatory GTPase ARF6 [ARF6(T27N)] in cultured adipocytes selectively inhibited both F-actin formation and GLUT4 translocation in response to endothelin 1 but not insulin. These data indicate that ARF6 is a required downstream element in endothelin 1 signaling through Galpha 11 to regulate cortical actin and GLUT4 translocation in cultured adipocytes, while insulin action involves different signaling pathways.

* Corresponding author. Mailing address: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation St., Worcester, MA 01605. Phone: (508) 856-2254. Fax: (508) 856-1617. E-mail: Michael.Czech{at}umassmed.edu.

dagger Present address: Office of Generic Drugs, Food and Drug Administration, Rockville, MD 20855-2773.

Molecular and Cellular Biology, August 2001, p. 5262-5275, Vol. 21, No. 15
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.15.5262-5275.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Shi, J., Huang, G., Kandror, K. V. (2008). Self-assembly of Glut4 Storage Vesicles during Differentiation of 3T3-L1 Adipocytes. J. Biol. Chem. 283: 30311-30321 [Abstract] [Full Text]  
  • D'Eon, T. M., Pierce, K. A., Roix, J. J., Tyler, A., Chen, H., Teixeira, S. R. (2008). The Role of Adipocyte Insulin Resistance in the Pathogenesis of Obesity-Related Elevations in Endocannabinoids. Diabetes 57: 1262-1268 [Abstract] [Full Text]  
  • Lauritzen, H. P.M.M., Galbo, H., Brandauer, J., Goodyear, L. J., Ploug, T. (2008). Large GLUT4 Vesicles Are Stationary While Locally and Reversibly Depleted During Transient Insulin Stimulation of Skeletal Muscle of Living Mice: Imaging Analysis of GLUT4-Enhanced Green Fluorescent Protein Vesicle Dynamics. Diabetes 57: 315-324 [Abstract] [Full Text]  
  • Li, J., Peters, P. J., Bai, M., Dai, J., Bos, E., Kirchhausen, T., Kandror, K. V., Hsu, V. W. (2007). An ACAP1-containing clathrin coat complex for endocytic recycling. JCB 178: 453-464 [Abstract] [Full Text]  
  • Hatanaka, T., Hatanaka, Y., Tsuchida, J.-i., Ganapathy, V., Setou, M. (2006). Amino Acid Transporter ATA2 Is Stored at the trans-Golgi Network and Released by Insulin Stimulus in Adipocytes. J. Biol. Chem. 281: 39273-39284 [Abstract] [Full Text]  
  • Hatanaka, T., Hatanaka, Y., Setou, M. (2006). Regulation of Amino Acid Transporter ATA2 by Ubiquitin Ligase Nedd4-2. J. Biol. Chem. 281: 35922-35930 [Abstract] [Full Text]  
  • McCarthy, A. M., Spisak, K. O., Brozinick, J. T., Elmendorf, J. S. (2006). Loss of cortical actin filaments in insulin-resistant skeletal muscle cells impairs GLUT4 vesicle trafficking and glucose transport. Am. J. Physiol. Cell Physiol. 291: C860-C868 [Abstract] [Full Text]  
  • Hou, J. C., Shigematsu, S., Crawford, H. C., Anastasiadis, P. Z., Pessin, J. E. (2006). Dual Regulation of Rho and Rac by p120 Catenin Controls Adipocyte Plasma Membrane Trafficking. J. Biol. Chem. 281: 23307-23312 [Abstract] [Full Text]  
  • Chen, G., Liu, P., Pattar, G. R., Tackett, L., Bhonagiri, P., Strawbridge, A. B., Elmendorf, J. S. (2006). Chromium Activates Glucose Transporter 4 Trafficking and Enhances Insulin-Stimulated Glucose Transport in 3T3-L1 Adipocytes via a Cholesterol-Dependent Mechanism. Mol. Endocrinol. 20: 857-870 [Abstract] [Full Text]  
  • Jaworski, J., Spangler, S., Seeburg, D. P., Hoogenraad, C. C., Sheng, M. (2005). Control of Dendritic Arborization by the Phosphoinositide-3'-Kinase-Akt-Mammalian Target of Rapamycin Pathway. J. Neurosci. 25: 11300-11312 [Abstract] [Full Text]  
  • Bose, A., Guilherme, A., Huang, S., Hubbard, A. C., Lane, C. R., Soriano, N. A., Czech, M. P. (2005). The v-SNARE Vti1a Regulates Insulin-stimulated Glucose Transport and Acrp30 Secretion in 3T3-L1 Adipocytes. J. Biol. Chem. 280: 36946-36951 [Abstract] [Full Text]  
  • Wettschureck, N., Offermanns, S. (2005). Mammalian G Proteins and Their Cell Type Specific Functions. Physiol. Rev. 85: 1159-1204 [Abstract] [Full Text]  
  • Huang, P., Altshuller, Y. M., Hou, J. C., Pessin, J. E., Frohman, M. A. (2005). Insulin-stimulated Plasma Membrane Fusion of Glut4 Glucose Transporter-containing Vesicles Is Regulated by Phospholipase D1. Mol. Biol. Cell 16: 2614-2623 [Abstract] [Full Text]  
  • Brozinick, J. T. Jr., Hawkins, E. D., Strawbridge, A. B., Elmendorf, J. S. (2004). Disruption of Cortical Actin in Skeletal Muscle Demonstrates an Essential Role of the Cytoskeleton in Glucose Transporter 4 Translocation in Insulin-sensitive Tissues. J. Biol. Chem. 279: 40699-40706 [Abstract] [Full Text]  
  • Chen, G., Raman, P., Bhonagiri, P., Strawbridge, A. B., Pattar, G. R., Elmendorf, J. S. (2004). Protective Effect of Phosphatidylinositol 4,5-Bisphosphate against Cortical Filamentous Actin Loss and Insulin Resistance Induced by Sustained Exposure of 3T3-L1 Adipocytes to Insulin. J. Biol. Chem. 279: 39705-39709 [Abstract] [Full Text]  
  • Funaki, M., Randhawa, P., Janmey, P. A. (2004). Separation of Insulin Signaling into Distinct GLUT4 Translocation and Activation Steps. Mol. Cell. Biol. 24: 7567-7577 [Abstract] [Full Text]  
  • Kanzaki, M., Furukawa, M., Raab, W., Pessin, J. E. (2004). Phosphatidylinositol 4,5-Bisphosphate Regulates Adipocyte Actin Dynamics and GLUT4 Vesicle Recycling. J. Biol. Chem. 279: 30622-30633 [Abstract] [Full Text]  
  • Guilherme, A., Soriano, N. A., Bose, S., Holik, J., Bose, A., Pomerleau, D. P., Furcinitti, P., Leszyk, J., Corvera, S., Czech, M. P. (2004). EHD2 and the Novel EH Domain Binding Protein EHBP1 Couple Endocytosis to the Actin Cytoskeleton. J. Biol. Chem. 279: 10593-10605 [Abstract] [Full Text]  
  • Liu, P., Leffler, B. J., Weeks, L. K., Chen, G., Bouchard, C. M., Strawbridge, A. B., Elmendorf, J. S. (2004). Sphingomyelinase activates GLUT4 translocation via a cholesterol-dependent mechanism. Am. J. Physiol. Cell Physiol. 286: C317-C329 [Abstract] [Full Text]  
  • Bergandi, L., Silvagno, F., Russo, I., Riganti, C., Anfossi, G., Aldieri, E., Ghigo, D., Trovati, M., Bosia, A. (2003). Insulin Stimulates Glucose Transport Via Nitric Oxide/Cyclic GMP Pathway in Human Vascular Smooth Muscle Cells. Arterioscler. Thromb. Vasc. Bio. 23: 2215-2221 [Abstract] [Full Text]  
  • Arnaoutova, I., Jackson, C. L., Al-Awar, O. S., Donaldson, J. G., Loh, Y. P. (2003). Recycling of Raft-associated Prohormone Sorting Receptor Carboxypeptidase E Requires Interaction with ARF6. Mol. Biol. Cell 14: 4448-4457 [Abstract] [Full Text]  
  • Donaldson, J. G. (2003). Multiple Roles for Arf6: Sorting, Structuring, and Signaling at the Plasma Membrane. J. Biol. Chem. 278: 41573-41576 [Full Text]  
  • Rachdaoui, N., Nagy, L. E. (2003). Endothelin-1-stimulated glucose uptake is desensitized by tumor necrosis factor-{alpha} in 3T3-L1 adipocytes. Am. J. Physiol. Endocrinol. Metab. 285: E545-E551 [Abstract] [Full Text]  
  • Rachdaoui, N., Sebastian, B. M., Nagy, L. E. (2003). Chronic ethanol feeding impairs endothelin-1-stimulated glucose uptake via decreased G{alpha}11 expression in rat adipocytes. Am. J. Physiol. Endocrinol. Metab. 285: E303-E310 [Abstract] [Full Text]  
  • Usui, I., Imamura, T., Huang, J., Satoh, H., Olefsky, J. M. (2003). Cdc42 Is a Rho GTPase Family Member That Can Mediate Insulin Signaling to Glucose Transport in 3T3-L1 Adipocytes. J. Biol. Chem. 278: 13765-13774 [Abstract] [Full Text]  
  • Jiang, Z. Y., Chawla, A., Bose, A., Way, M., Czech, M. P. (2002). A Phosphatidylinositol 3-Kinase-independent Insulin Signaling Pathway to N-WASP/Arp2/3/F-actin Required for GLUT4 Glucose Transporter Recycling. J. Biol. Chem. 277: 509-515 [Abstract] [Full Text]  
  • Park, J. G., Bose, A., Leszyk, J., Czech, M. P. (2001). PYK2 as a Mediator of Endothelin-1/Galpha 11 Signaling to GLUT4 Glucose Transporters. J. Biol. Chem. 276: 47751-47754 [Abstract] [Full Text]  
  • Claing, A., Chen, W., Miller, W. E., Vitale, N., Moss, J., Premont, R. T., Lefkowitz, R. J. (2001). beta -Arrestin-mediated ADP-ribosylation Factor 6 Activation and beta 2-Adrenergic Receptor Endocytosis. J. Biol. Chem. 276: 42509-42513 [Abstract] [Full Text]  
  • Lawrence, J. T. R., Birnbaum, M. J. (2001). ADP-Ribosylation Factor 6 Delineates Separate Pathways Used by Endothelin 1 and Insulin for Stimulating Glucose Uptake in 3T3-L1 Adipocytes. Mol. Cell. Biol. 21: 5276-5285 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»