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Molecular and Cellular Biology, September 2001, p. 5826-5837, Vol. 21, No. 17
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.17.5826-5837.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Transcriptional Activation Domains of Human Heat Shock Factor 1 Recruit Human SWI/SNF

E. Kelly Sullivan,1,2 Christine S. Weirich,1,2,dagger Jeffrey R. Guyon,1,2 Saïd Sif,1,2,Dagger and Robert E. Kingston1,2,*

Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114,1 and Department of Genetics, Harvard Medical School, Boston, Massachusetts 021152

Received 12 February 2001/Returned for modification 21 March 2001/Accepted 7 June 2001

Chromatin remodeling complexes such as SWI/SNF use the energy of ATP hydrolysis to remodel nucleosomal DNA and increase transcription of nucleosomal templates. Human heat shock factor one (hHSF1) is a tightly regulated activator that stimulates transcriptional initiation and elongation using different portions of its activation domains. Here we demonstrate that hHSF1 associates with BRG1, the ATPase subunit of human SWI/SNF (hSWI/SNF) at endogenous protein concentrations. We also show that hHSF1 activation domains recruit hSWI/SNF to a chromatin template in a purified system. Mutation of hHSF1 residues responsible for activation of transcriptional elongation has the most severe effect on recruitment of SWI/SNF and association of hHSF1 with BRG1, suggesting that recruitment of chromatin remodeling activity might play a role in stimulation of elongation.

* Corresponding author. Mailing address: Department of Molecular Biology, Wellman 10, Massachusetts General Hospital, Boston, MA 02114. Phone: (617) 726-5990. Fax: (617) 726-5949. E-mail: kingston{at}frodo.mgh.harvard.edu.

dagger Present address: Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, Calif.

Dagger Present address: Department of Molecular and Cellular Biochemistry, Ohio State University College of Medicine, Columbus, Ohio.

Molecular and Cellular Biology, September 2001, p. 5826-5837, Vol. 21, No. 17
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.17.5826-5837.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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