This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Plant, K. E.
Right arrow Articles by Proudfoot, N. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Plant, K. E.
Right arrow Articles by Proudfoot, N. J.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2001, p. 6507-6514, Vol. 21, No. 19
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.19.6507-6514.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Intergenic Transcription in the Human beta -Globin Gene Cluster

Kathryn E. Plant, Samantha J. E. Routledge, and Nick J. Proudfoot*

Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom

Received 29 January 2001/Returned for modification 9 May 2001/Accepted 2 July 2001

Our previous studies on nascent transcription across the human beta -globin gene cluster revealed the presence of intergenic transcripts in addition to the expected genic transcripts. We now show that transcription into the beta -globin locus control region (LCR) begins within an ERV9 endogenous retroviral long terminal repeat upstream of DNase I hypersensitive site 5. However, in a transgenic mouse, which has the human beta -globin LCR but lacks the ERV9 LTR, transcription begins upstream of the transgenic locus. We postulate that in this transgenic mouse nearby endogenous mouse promoters are activated by the LCR. Intergenic transcription is also detected across the whole transgenic globin gene locus independently of the stage of erythroid development. Intergenic transcription in the beta -globin cluster is erythroid specific; however, it can be induced in nonerythroid cells by several means: by transinduction with a plasmid transcribing part of the cluster, by exogenous addition of transcription factors, and by treatment with the histone deacetylase inhibitor trichostatin A.

* Corresponding author. Mailing address: Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom. Phone: 44-(0)1865 275566. Fax: 44-(0)1865 275556. E-mail: nicholas.proudfoot{at}path.ox.ac.uk.

Molecular and Cellular Biology, October 2001, p. 6507-6514, Vol. 21, No. 19
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.19.6507-6514.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Beauchemin, H., Trudel, M. (2009). Evidence for a Bigenic Chromatin Subdomain in Regulation of the Fetal-to-Adult Hemoglobin Switch. Mol. Cell. Biol. 29: 1635-1648 [Abstract] [Full Text]  
  • Shimotsuma, M., Matsuzaki, H., Tanabe, O., Campbell, A. D., Engel, J. D., Fukamizu, A., Tanimoto, K. (2007). Linear Distance from the Locus Control Region Determines {varepsilon}-Globin Transcriptional Activity. Mol. Cell. Biol. 27: 5664-5672 [Abstract] [Full Text]  
  • Zhao, H., Friedman, R. D., Fournier, R. E. K. (2007). The Locus Control Region Activates Serpin Gene Expression through Recruitment of Liver-Specific Transcription Factors and RNA Polymerase II. Mol. Cell. Biol. 27: 5286-5295 [Abstract] [Full Text]  
  • Rybtsova, N., Leimgruber, E., Seguin-Estevez, Q., Dunand-Sauthier, I., Krawczyk, M., Reith, W. (2007). Transcription-coupled deposition of histone modifications during MHC class II gene activation. Nucleic Acids Res 35: 3431-3441 [Abstract] [Full Text]  
  • Kim, A., Kiefer, C. M., Dean, A. (2007). Distinctive Signatures of Histone Methylation in Transcribed Coding and Noncoding Human {beta}-Globin Sequences. Mol. Cell. Biol. 27: 1271-1279 [Abstract] [Full Text]  
  • Prasanth, K. V., Spector, D. L. (2007). Eukaryotic regulatory RNAs: an answer to the 'genome complexity' conundrum. Genes Dev. 21: 11-42 [Abstract] [Full Text]  
  • Lee, Y., Ise, T., Ha, D., Saint Fleur, A., Hahn, Y., Liu, X.-F., Nagata, S., Lee, B., Bera, T. K., Pastan, I. (2006). Evolution and expression of chimeric POTE-actin genes in the human genome. Proc. Natl. Acad. Sci. USA 103: 17885-17890 [Abstract] [Full Text]  
  • Bank, A. (2006). Regulation of human fetal hemoglobin: new players, new complexities. Blood 107: 435-443 [Abstract] [Full Text]  
  • DYE, M.J., GROMAK, N., HAUSSECKER, D., WEST, S., PROUDFOOT, N.J. (2006). Turnover and Function of Noncoding RNA Polymerase II Transcripts. Cold Spring Harb Symp Quant Biol 71: 275-284 [Abstract]  
  • Haussecker, D., Proudfoot, N. J. (2005). Dicer-Dependent Turnover of Intergenic Transcripts from the Human {beta}-Globin Gene Cluster. Mol. Cell. Biol. 25: 9724-9733 [Abstract] [Full Text]  
  • Feng, Y.-Q., Warin, R., Li, T., Olivier, E., Besse, A., Lobell, A., Fu, H., Lin, C. M., Aladjem, M. I., Bouhassira, E. E. (2005). The Human {beta}-Globin Locus Control Region Can Silence as Well as Activate Gene Expression. Mol. Cell. Biol. 25: 3864-3874 [Abstract] [Full Text]  
  • Plant, K. E., Dye, M. J., Lafaille, C., Proudfoot, N. J. (2005). Strong Polyadenylation and Weak Pausing Combine To Cause Efficient Termination of Transcription in the Human G{gamma}-Globin Gene. Mol. Cell. Biol. 25: 3276-3285 [Abstract] [Full Text]  
  • Kalmykova, A. I., Nurminsky, D. I., Ryzhov, D. V., Shevelyov, Y. Y. (2005). Regulated chromatin domain comprising cluster of co-expressed genes in Drosophila melanogaster. Nucleic Acids Res 33: 1435-1444 [Abstract] [Full Text]  
  • Szutorisz, H., Canzonetta, C., Georgiou, A., Chow, C.-M., Tora, L., Dillon, N. (2005). Formation of an Active Tissue-Specific Chromatin Domain Initiated by Epigenetic Marking at the Embryonic Stem Cell Stage. Mol. Cell. Biol. 25: 1804-1820 [Abstract] [Full Text]  
  • Ling, J., Ainol, L., Zhang, L., Yu, X., Pi, W., Tuan, D. (2004). HS2 Enhancer Function Is Blocked by a Transcriptional Terminator Inserted between the Enhancer and the Promoter. J. Biol. Chem. 279: 51704-51713 [Abstract] [Full Text]  
  • Krawczyk, M., Peyraud, N., Rybtsova, N., Masternak, K., Bucher, P., Barras, E., Reith, W. (2004). Long Distance Control of MHC Class II Expression by Multiple Distal Enhancers Regulated by Regulatory Factor X Complex and CIITA. J. Immunol. 173: 6200-6210 [Abstract] [Full Text]  
  • Castelo-Branco, P., Furger, A., Wollerton, M., Smith, C., Moreira, A., Proudfoot, N. (2004). Polypyrimidine Tract Binding Protein Modulates Efficiency of Polyadenylation. Mol. Cell. Biol. 24: 4174-4183 [Abstract] [Full Text]  
  • Kim, A., Dean, A. (2004). Developmental stage differences in chromatin subdomains of the {beta}-globin locus. Proc. Natl. Acad. Sci. USA 101: 7028-7033 [Abstract] [Full Text]  
  • Rogan, D. F., Cousins, D. J., Santangelo, S., Ioannou, P. A., Antoniou, M., Lee, T. H., Staynov, D. Z. (2004). Analysis of intergenic transcription in the human IL-4/IL-13 gene cluster. Proc. Natl. Acad. Sci. USA 101: 2446-2451 [Abstract] [Full Text]  
  • Tanimoto, K., Sugiura, A., Omori, A., Felsenfeld, G., Engel, J. D., Fukamizu, A. (2003). Human {beta}-Globin Locus Control Region HS5 Contains CTCF- and Developmental Stage-Dependent Enhancer-Blocking Activity in Erythroid Cells. Mol. Cell. Biol. 23: 8946-8952 [Abstract] [Full Text]  
  • van Driel, R., Fransz, P. F., Verschure, P. J. (2003). The eukaryotic genome: a system regulated at different hierarchical levels. J. Cell Sci. 116: 4067-4075 [Abstract] [Full Text]  
  • Johnson, K. D., Grass, J. A., Park, C., Im, H., Choi, K., Bresnick, E. H. (2003). Highly Restricted Localization of RNA Polymerase II within a Locus Control Region of a Tissue-Specific Chromatin Domain. Mol. Cell. Biol. 23: 6484-6493 [Abstract] [Full Text]  
  • Ling, J., Pi, W., Yu, X., Bengra, C., Long, Q., Jin, H., Seyfang, A., Tuan, D. (2003). The ERV-9 LTR enhancer is not blocked by the HS5 insulator and synthesizes through the HS5 site non-coding, long RNAs that regulate LTR enhancer function. Nucleic Acids Res 31: 4582-4596 [Abstract] [Full Text]  
  • Gaensler, K. M. L., Zhang, Z., Lin, C., Yang, S., Hardt, K., Flebbe-Rehwaldt, L. (2003). Sequences in the Agamma -delta intergenic region are not required for stage-specific regulation of the human beta -globin gene locus. Proc. Natl. Acad. Sci. USA 100: 3374-3379 [Abstract] [Full Text]  
  • Johnson, K. D., Grass, J. A., Boyer, M. E., Kiekhaefer, C. M., Blobel, G. A., Weiss, M. J., Bresnick, E. H. (2002). Cooperative activities of hematopoietic regulators recruit RNA polymerase II to a tissue-specific chromatin domain. Proc. Natl. Acad. Sci. USA 99: 11760-11765 [Abstract] [Full Text]  
  • Farrell, C. M., West, A. G., Felsenfeld, G. (2002). Conserved CTCF Insulator Elements Flank the Mouse and Human {beta}-Globin Loci. Mol. Cell. Biol. 22: 3820-3831 [Abstract] [Full Text]  
  • Storz, G. (2002). An Expanding Universe of Noncoding RNAs. Science 296: 1260-1263 [Abstract] [Full Text]  
  • Hogga, I., Karch, F. (2002). Transcription through the iab-7 cis-regulatory domain of the bithorax complex interferes with maintenance of Polycomb-mediated silencing. Development 129: 4915-4922 [Abstract] [Full Text]  
  • Bender, W., Fitzgerald, D. P. (2002). Transcription activates repressed domains in the Drosophila bithorax complex. Development 129: 4923-4930 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»