This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kirchner, J.
Right arrow Articles by Weil, P. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kirchner, J.
Right arrow Articles by Weil, P. A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2001, p. 6668-6680, Vol. 21, No. 19
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.19.6668-6680.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular Genetic Dissection of TAF25, an Essential Yeast Gene Encoding a Subunit Shared by TFIID and SAGA Multiprotein Transcription Factors

Jay Kirchner, Steven L. Sanders, Edward Klebanow,dagger and P. Anthony Weil*

Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0615

Received 30 April 2001/Returned for modification 31 May 2001/Accepted 27 June 2001

We have performed a systematic structure-function analysis of Saccharomyces cerevisiae TAF25, an evolutionarily conserved, single-copy essential gene which encodes the 206-amino-acid TAF25p protein. TAF25p is an integral subunit of both the 15-subunit general transcription factor TFIID and the multisubunit, chromatin-acetylating transcriptional coactivator SAGA. We used hydroxylamine mutagenesis, targeted deletion, alanine-scanning mutagenesis, high-copy suppression methods, and two-hybrid screening to dissect TAF25. Temperature-sensitive mutant strains generated were used for coimmunoprecipitation and transcription analyses to define the in vivo functions of TAF25p. The results of these analyses show that TAF25p is comprised of multiple mutable elements which contribute importantly to RNA polymerase II-mediated mRNA gene transcription.


* Corresponding author. Mailing address: Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232-0615. Phone: (615) 322-7007. Fax: (615) 322-7236. E-mail: tony.weil{at}mcmail.vanderbilt.edu.

dagger Present address: Argus Research Corporation, New York, NY 10006.


Molecular and Cellular Biology, October 2001, p. 6668-6680, Vol. 21, No. 19
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.19.6668-6680.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Irvin, J. D., Pugh, B. F. (2006). Genome-wide Transcriptional Dependence on TAF1 Functional Domains. J. Biol. Chem. 281: 6404-6412 [Abstract] [Full Text]  
  • Frontini, M., Soutoglou, E., Argentini, M., Bole-Feysot, C., Jost, B., Scheer, E., Tora, L. (2005). TAF9b (Formerly TAF9L) Is a Bona Fide TAF That Has Unique and Overlapping Roles with TAF9. Mol. Cell. Biol. 25: 4638-4649 [Abstract] [Full Text]  
  • Soutoglou, E., Demeny, M. A., Scheer, E., Fienga, G., Sassone-Corsi, P., Tora, L. (2005). The Nuclear Import of TAF10 Is Regulated by One of Its Three Histone Fold Domain-Containing Interaction Partners. Mol. Cell. Biol. 25: 4092-4104 [Abstract] [Full Text]  
  • Mohan, W. S. II, Scheer, E., Wendling, O., Metzger, D., Tora, L. (2003). TAF10 (TAFII30) Is Necessary for TFIID Stability and Early Embryogenesis in Mice. Mol. Cell. Biol. 23: 4307-4318 [Abstract] [Full Text]  
  • Kobayashi, A., Miyake, T., Kawaichi, M., Kokubo, T. (2003). Mutations in the histone fold domain of the TAF12 gene show synthetic lethality with the TAF1 gene lacking the TAF N-terminal domain (TAND) by different mechanisms from those in the SPT15 gene encoding the TATA box-binding protein (TBP). Nucleic Acids Res 31: 1261-1274 [Abstract] [Full Text]  
  • Thuault, S., Gangloff, Y.-G., Kirchner, J., Sanders, S., Werten, S., Romier, C., Weil, P. A., Davidson, I. (2002). Functional Analysis of the TFIID-specific Yeast TAF4 (yTAFII48) Reveals an Unexpected Organization of Its Histone-fold Domain. J. Biol. Chem. 277: 45510-45517 [Abstract] [Full Text]  
  • Sanders, S. L., Garbett, K. A., Weil, P. A. (2002). Molecular Characterization of Saccharomyces cerevisiae TFIID. Mol. Cell. Biol. 22: 6000-6013 [Abstract] [Full Text]  
  • Sanders, S. L., Jennings, J., Canutescu, A., Link, A. J., Weil, P. A. (2002). Proteomics of the Eukaryotic Transcription Machinery: Identification of Proteins Associated with Components of Yeast TFIID by Multidimensional Mass Spectrometry. Mol. Cell. Biol. 22: 4723-4738 [Abstract] [Full Text]  
  • Kirschner, D. B., vom Baur, E., Thibault, C., Sanders, S. L., Gangloff, Y.-G., Davidson, I., Weil, P. A., Tora, L. (2002). Distinct Mutations in Yeast TAFII25 Differentially Affect the Composition of TFIID and SAGA Complexes as Well as Global Gene Expression Patterns. Mol. Cell. Biol. 22: 3178-3193 [Abstract] [Full Text]